During the past 20 years, the use of protective gloves in health care has increased, as have hypersensitivity reactions to the natural rubber latex (NRL) used in glove manufacture. A study was conducted to determine what nurses who work in environments in which NRL is commonly used know about NRL, including their education about and experience with NRL-related allergies. Nurses replied to a questionnaire with 20 statements pertaining to NRL and NRL-related allergies. The results showed that the nurses had inadequate knowledge about NRL, but nurses in a specialty training program scored significantly higher than professionally active nurses.