Exercise-associated hyponatremia occurs in horses and humans, both species that sweat, and in sled dogs, which do not sweat. To investigate the mechanism of exercise-associated hyponatremia in sled dogs, we measured water turnover, serum electrolyte concentrations and osmolality, plasma renal hormone concentrations, and urine composition of 12 fit Alaskan sled dogs before, during, and after a 490-km sled dog race (Ex group). Water turnover and serum electrolyte concentrations were measured in six similarly fit dogs that did not run (Sed group). Water turnover was significantly larger (P