This article is devoted to current issues of human resources development in healthcare. The article emphasizes that the effectiveness of the healthcare system and the quality of medical care delivery deeply depend on the results of the work of medical workers, which are determined by their knowledge, skills and motivation. Considering the necessity of providing the healthcare sector with highly qualified personnel, as well as replenishing the staff with young specialists, we can say that public health is directly dependent on their competence. In addition, the problem of reducing staff turnover and retaining young specialists in the workplace is important, this problem can be solved by the mentoring system in a medical organization. In the article documents of international organizations and the Russian regulatory framework are analyzed, the data from the author's studies conducted in 2019-2020 are presented. The results of the expert interviews and questionnaires indicate a positive attitude of the respondents to the mentoring system as an instrument for developing human resources in medical organizations. Analyzing the data obtained, the author comes to the conclusion about the need for a competent organization of the mentoring process. This article focuses on the legislative regulation of mentoring, as well as incentive measures for mentors.