Objective We previously found that administering a pre-screening lifestyle questionnaire lowered the subsequent attendance proportion in the first-ever colorectal cancer screening. We sought to determine whether the effect continued in subsequent screening rounds. Methods The eligible survey cohort ( n?=?10,375) received a follow-up questionnaire in 2012, and in 2013, they were invited for colorectal cancer screening for the second time. For the third screening round, in 2015, no questionnaires were sent in the previous year. Screening attendance in 2013 and in 2015 was examined in relation to survey mailings. Results The colorectal cancer screening attendance rate in 2013 was 58.4% in the survey population, and 63.9% in those not surveyed ( P?