In this study, the bacterial populations of roots and mycospheres of the boreal pine forest ericoid plants, heather (Calluna vulgaris), bilberry (Vaccinium myrtillus), and lingonberry (Vaccinium vitis-idaea), were studied by qPCR and next-generation sequencing (NGS). All bacterial communities of mycosphere soils differed from soils uncolonized by mycorrhizal mycelia. Colonization by mycorrhizal hyphae increased the total number of bacterial 16S ribosomal DNA (rDNA) gene copies in the humus but decreased the number of different bacterial operational taxonomic units (OTUs). Nevertheless, ericoid roots and mycospheres supported numerous OTUs not present in uncolonized humus. Bacterial communities in bilberry mycospheres were surprisingly similar to those in pine mycospheres but not to bacterial communities in heather and lingonberry mycospheres. In contrast, bacterial communities of ericoid roots were more similar to each other than to those of pine roots. In all sample types, the relative abundances of bacterial sequences belonging to Alphaproteobacteria and Acidobacteria were higher than the sequences belonging to other classes. Soil samples contained more Actinobacteria, Deltaproteobacteria, Opitutae, and Planctomycetia, whereas Armatimonadia, Betaproteobacteria, Gammaproteobacteria, and Sphingobacteriia were more common to roots. All mycosphere soils and roots harbored bacteria unique to that particular habitat. Our study suggests that the habitation by ericoid plants increases the overall bacterial diversity of boreal forest soils.
Notes
Cites: ISME J. 2008 Aug;2(8):887-90018421258
Cites: PLoS One. 2014 Jan 28;9(1):e8721724489873
Cites: Nat Commun. 2014 Sep 18;5:495025232638
Cites: PLoS One. 2013;8(2):e5792323460914
Cites: Environ Microbiol. 2011 Mar;13(3):819-3021176055
Cites: Microb Ecol. 2010 Feb;59(2):335-4319705192
Cites: Appl Environ Microbiol. 2009 Apr;75(7):1979-8919181827
Cites: BMC Microbiol. 2013 Sep 11;13:20524025151
Cites: FEMS Microbiol Lett. 2010 Oct;311(2):103-1220840602
Cites: Can J Microbiol. 2006 Aug;52(8):769-7816917536
Cites: Science. 2002 May 10;296(5570):1064-612004116
Cites: Nucleic Acids Res. 1980 Oct 10;8(19):4321-57433111
Cites: Oecologia. 2003 Oct;137(2):252-712883986
Cites: Bioinformatics. 2011 Aug 15;27(16):2194-20021700674