A novel Gram-stain-positive, spore-forming, aerobic, motile and rod-shaped bacterium designated strain PAMC 80007T was isolated from an active layer soil sample of Council, Alaska. Optimal growth of strain PAMC 80007T was observed at 30?°C, pH?7.0 and in the presence of 2?% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain PAMC 80007T belonged to the genus Domibacillus. This strain was closely related to Domibacillus enclensis (98.3?%), Domibacillus robiginosus (98.3?%) and Domibacillus indicus (97.2?%). Genomic DNA G+C content was 43.5?mol% and genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 80007T is clearly distinguished from the closely related species of the genus Domibacillus. The major fatty acids (>5?%) were iso-C15?:?0 (24.7?%), C16?:?1?11c (16.8?%), anteiso-C15?:?0 (16.5?%), C16?:?0 (15.6?%) and anteiso-C17?:?0 (8.7?%). The major respiratory isoprenoid quinones were menaquinone-6 (MK-6) and menaquinone-7 (MK-7), and the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, phospholipid and two unidentified lipids. meso-Diaminopimelic acid (type A1?) was present in the cell-wall peptidoglycan, and the major whole-cell sugar was ribose with a minor quantity of glucose. Results from a polyphasic study suggested that strain PAMC 80007T represents a novel species of the genus Domibacillus for which the name Domibacillus tundrae sp. nov. is proposed. The type strain is PAMC 80007T (?=?JCM 30371T?=?KCTC 33549T?=?DSM 29572T). An emended description of the genus Domibacillus is also provided.