The parameters of vanadium determination by ICP-MS in whole blood are presented. Conditions for blood sample preparation to reduce measure errors and to determine vanadium at the reference concentration level were optimized. The accuracy of the results is confirmed by analysis of standard blood samples Seronorm L1, L2 and L3. Vanadium mean in whole blood for the group of children from the town of Chusovoy (n = 80) was 1.29 +/- 0.45 microg/L, and vanadium mean for grown-ups from the town of Chusovoy was 1.63 +/- 0.25 microg/L.