The effects of CD8+gammadelta T cells on late allergic airway responses and airway inflammation in rats.
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Publication Type
Susumu Isogai
Alexandra Rubin
Karim Maghni
David Ramos-Barbon
Rame Taha
Yasuyuki Yoshizawa
Qutayba Hamid
James G Martin
Author Affiliation
Meakins Christie Laboratories, Department of Medicine, McGill University, 3623 St Urbain, Montreal, Quebec, Canada H2X 2P2.
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Publication Type
Adoptive Transfer
Allergens - administration & dosage
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis - genetics
Eosinophilia - immunology
Immunoglobulin E - blood
Interferon Type II - biosynthesis - genetics
Interleukin-4 - biosynthesis - genetics
Models, Immunological
Ovalbumin - administration & dosage - immunology
RNA, Messenger - genetics - metabolism
Rats, Inbred BN
Receptors, Antigen, T-Cell, gamma-delta - metabolism
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - etiology - immunology
T-Lymphocyte Subsets - immunology
BACKGROUND: Gamma-delta (gammadelta) T cells regulate immune responses to foreign protein at mucosal surfaces. Whether they can modify allergen-induced early (EAR) and late airway responses (LAR) is unknown. OBJECTIVE: We have tested the hypothesis that the CD8+ subtype of gammadelta T cells decreases allergen-induced LAR and airway eosinophilia in the rat. METHODS: Brown Norway rats were administered, intraperitoneally, 3.5 x 10(4) lymph node CD8+gammadelta T cells from naive or sensitized rats. The recipients were sensitized to ovalbumin (OVA) in Al(OH)(3) 3 days after cell transfer and challenged with aerosolized OVA 14 days later. Serum IgE was measured before allergen challenge. After challenge, lung resistance was monitored for 8 hours and then bronchoalveolar lavage (BAL) was analyzed for eosinophil major basic protein (MBP), IL-4, IL-5, IL-13, and IFN-gamma messenger RNA-expressing cells. RESULTS: gammadelta T cells from naive donors significantly decreased LAR in OVA-challenged sensitized rats, whereas MBP(+) eosinophils were decreased by both gammadelta T cells from naive and sensitized donors. EAR and serum IgE levels were unchanged. The expression of IL-4, IL-5, and IL-13 by BAL cells of gammadelta T cell recipients was attenuated compared with OVA-challenged controls. This was accompanied by an increase in the expression of IFN-gamma. CONCLUSIONS: Our results are consistent with a suppressive role of CD8+gammadelta T cells on allergic airway responses. However, only gammadelta T cells from naive donors inhibit LAR.
PubMed ID
13679814 View in PubMed
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