New Arctic Bacterial Isolates with Relevant Enzymatic Potential.

https://arctichealth.org/en/permalink/ahliterature304889
Source
Molecules. 2020 Aug 28; 25(17):
Publication Type
Journal Article
Date
Aug-28-2020
Author
Michal Piegza
Wojciech Laba
Miroslava Kacániová
Author Affiliation
Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, 51-630 Wroclaw, Poland.
Source
Molecules. 2020 Aug 28; 25(17):
Date
Aug-28-2020
Language
English
Publication Type
Journal Article
Abstract
Fragments of wood drifting in the vicinity of Spitzbergen were used for the isolation of microorganisms, carried out using atypical carbon sources: colloidal chitin, cellulose and carboxymethylcellulose, xylan, casein, tributrin and olive oil. Purified cultures were subjected to a three-step identification: with classical methods, using MALDI-TOF MS Biotyper whole-cell protein fingerprinting, and molecular analysis of 16S rDNA. Subsequently, a preliminary assessment of the enzymatic potential of isolates was carried out. As a result, cellulolytic activity was observed in more than 50% of the bacterial strains, exhibiting activity of 0.30-0.40 U/mL. Over 53% of the isolates demonstrated xylanolytic activity, of which the highest reached from 0.40 to 0.90 U. Polygalacturonase activity of 0.003-1.6 was also demonstrated in half of the bacterial strains studied. Proteolytic activity of isolates did not exceed 0.3 U. An important highlight was the ability of fluorescent dye production by certain strains, grown on skim milk-agar, but also on pure meat extract.
PubMed ID
32872091 View in PubMed
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