Morphological Evaluation of Oxidative Phosphorylation System in Myocardial Infarction under Conditions of Modified Vascular Endothelial Growth Factor Concentration.

https://arctichealth.org/en/permalink/ahliterature264990
Source
Bull Exp Biol Med. 2015 Jul 25;
Publication Type
Article
Date
Jul-25-2015
Author
M G Shurygin
I A Shurygina
O V Kanya
N N Dremina
E L Lushnikova
R D Nepomnyashchikh
Source
Bull Exp Biol Med. 2015 Jul 25;
Date
Jul-25-2015
Language
English
Publication Type
Article
Abstract
The relationship between vascular endothelium growth factor (VEGF) and cardiomyocyte oxidative phosphorylation level in experimental myocardial infarction, caused by diathermocoagulation of the periconical ventricular artery, was studied by immunofluorescent microscopy. Staining showed uneven distribution of cytochrome c in the mitochondria in the focus of myocardial infarction 2 h after the operation. After 24 h uneven staining of cardiomyocytes was found in the peri-infarction zone and no staining in the zone of myocardial infarction. This indicated a significant decrease in the level of redox enzymes. This picture persisted till day 14. Intraventricular injection of VEGF to animals led to a significant increase of the immunohistochemical reaction intensity, which reached the peak by day 7. The distribution of immunohistochemical reaction products under conditions of VEGF blocking was about the same as in spontaneous postinfarction reparative restitution. Our data indicated that increase of VEGF concentration in the myocardium maintained and stimulated oxidative phosphorylation in cardiomyocytes during the postinfarction period.
PubMed ID
26205728 View in PubMed
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