OBJECTIVES: To assess whether lactate determination in vaginal fluid is associated with, and can predict, onset of labour for women with suspected prelabour rupture of the membranes (PROM). DESIGN: Prospective observational study. SETTING: Labour ward at Soder Hospital, Stockholm, Sweden. POPULATION: Women with suspected PROM after 34 weeks of gestation, who later had spontaneous onset of labour (n = 179). METHODS: All women underwent a speculum examination and a test for determining lactate concentration in vaginal fluid. We used logistic regression to estimate the association between lactate concentration in vaginal fluid and time to onset of labour. MAIN OUTCOME MEASURES: Time from examination to onset of labour (cervix > or =4 cm), within 24 hours and 48 hours. RESULTS: The median time interval between examination and spontaneous onset of labour was 8.4 hours for women with 'high' lactate (> or =4.5 mmol/l) and 54 hours for those with 'low' lactate concentrations ( or =4.5 mmol/l) in vaginal fluid can be used to predict whether a woman with suspected PROM will commence spontaneous onset of labour within 24 or 48 hours.