Negative Mood (NM) is a 19-items adjective check-list developed to assess negative mood and stress responses. The items of the scale reflect dimensions such as depressed mood, anxiety, anger and time urgency. The data were collected from four different samples, two random population samples and two smaller selective samples. The psychometric properties of the NM showed high internal consistency (Cronbach's Alpha). When analysed with principal components analysis, three factors emerged, anxiety/depression, time pressure, and anger. The factors emerged in all four samples, within samples and between gender. The NM factors were tested for convergent and discriminative validity by correlating them with other more established measures of different aspects of negative mood. The results showed high convergent and discriminative validity for two of the NM factors, i.e., anxiety/depression, and anger, whereas the results for the third factor, time pressure, were more ambiguous. This scale has proven to be useful in capturing some vital dimensions of negative affect across different kinds of populations.