Non-anadromous forms of Arctic char (Salvelinus alpinus), those that are restricted to lakes and rivers, typically have higher mercury concentrations than anadromous forms that migrate to and from the sea. Using tissue burden data from the literature and our own analyses, we performed a screening-level risk assessment of methylmercury for non-anadromous Arctic char. Our assessment included 1569 fish distributed across 83 sites. Site-specific mean total mercury concentrations in non-anadromous Arctic char muscle varied considerably from 0.01 to 1.13?µg/g wet weight (ww), with 21% (17 of 83 sites) meeting or exceeding a threshold-effect level in fish of 0.33?µg/g ww, and 13% (11 of 83 sites) meeting or exceeding a threshold-effect level in fish of 0.5?µg/g ww. Of the sites in exceedance of the 0.33?µg/g threshold, seven were located in Greenland and ten in Canada (Labrador, Nunavut, Yukon). All but one of these sites were located in interfrost or permafrost biomes. Maximum total mercury concentrations exceeded 0.33?µg/g ww at 53% of sites (40 of the 75 sites with available maximum mercury values), and exceeded 0.5?µg/g ww at 27% (20 of 75 sites). Collectively, these results indicate that certain populations of non-anadromous Arctic char located mainly in interfrost and permafrost regions may be at risk for methylmercury toxicity. This approach provides a simple statistical assessment of methylmercury risk to non-anadromous Arctic char, and does not indicate actual effects. We highlight the need for studies that evaluate the potential toxic effects of methylmercury in non-anadromous Arctic char, as well as those that aid in the development of a methylmercury toxic-effect threshold specific to this species of fish. This article is protected by copyright. All rights reserved.