The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells.
Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation.
Low-level laser (wavelength, 660?nm; power output, 50?mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production).
The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12?J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8?J/cm(2).
The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).