For detection Aleutian mink disease virus (AMDV) antibodies, an ELISA was developed using the recombinant VP2332-452 protein as an antigen. Counter immunoelectrophoresis (CIEP) was used as a reference test to compare the results of enzyme linked immunosorbent assay (ELISA) and western blotting (WB), the specificity and sensitivity of VP2332-452-ELISA was 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452-ELISA might be a preferable method for detecting antibodies against AMDV.