In the present article there are considered the features of the formation of post-vaccination immunity in dependence on the level of anthropogenic load. The level of anthropogenic load on urban and rural areas on the base of the database of the Regional Foundation for Socio-hygienic monitoring of the Orenburg region has been determined. The assessment of the state of post-vaccination immunity was performed on average long-term indices in the indicator groups of children and adults. The distribution of the population throughout the strength of immunity to measles in dependence on area of residence has been determined. The relationship between the formation of post-vaccination immunity area of the residence has been established. In the population residing in urban areas the number of post- vaccination antibodies was significantly lower than that of the population in rural settlements.