Patients and medical staff of hemodialysis centers were screened for HBV, HCV and HDV markers. The incidence of mixed infection in patients reached 34.8%, while in medical personnel it was only 3.4%. There was an increasing rate of HBV markers in persons who have been immunized with ENGERIX-B vaccine according to standard schedules one month, one year and three years after the vaccination. Administration of Cuban vaccine using modified schedules is perspective for wide immunoprophylaxis in medical staff. Immunization policy for hemodialysis patients needs further perfection.