Uranium is ubiquitously found in drinking water and food. The gastrointestinal tract absorption fraction (f(1)) is an important parameter in risk assessment of uranium burdens from ingestion. Although absorption of uranium from ingestion has been studied extensively in the past, human data concerning children and adults are still limited. In a previous study based on measurements of uranium concentration in 73 bone-ash samples collected by Health Canada, the absorption fractions for uranium ingestion were determined to be 0.093 ± 0.113 for infants, and 0.050 ± 0.032 for young children ranging from 1 to 7 y of age. To extend the study, a total of 69 bone-ash samples were selected for children and adults ranging from 7 to 25 y of age and residing in the same Canadian community that is known to have an elevated level of uranium in its drinking water supply. For each bone-ash sample, the total uranium concentration was measured by inductively coupled plasma mass spectrometry. To solve uranium transfer in the biokinetic model for uranium given in International Commission of Radiological Protection (ICRP) Publication 69 with estimated daily uranium intake, the program WinSAAM v3.0.1 was used. The absorption fractions were determined to be 0.030 ± 0.022 for children (7-18 y) and 0.021 ± 0.015 for adults (18-25 y). For anyone more than 18 y of age, the estimated f(1) value for uranium agree well with the ICRP recommended value of 0.02.