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Allergenic and immunogenic components of house dust mite, Dermatophagoides farinae.

https://arctichealth.org/en/permalink/ahliterature3845
Source
Ann Allergy. 1986 Feb;56(2):150-5
Publication Type
Article
Date
Feb-1986
Author
S. Nakada
M. Haida
T. Nakagawa
K. Ito
T. Miyamoto
Source
Ann Allergy. 1986 Feb;56(2):150-5
Date
Feb-1986
Language
English
Publication Type
Article
Keywords
Allergens
Animals
Antibodies, Anti-Idiotypic - analysis
Asthma - blood - immunology
Chromatography, Gel
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G - immunology
Mice
Mice, Inbred BALB C
Mites - immunology
Research Support, Non-U.S. Gov't
Skin Tests
Tissue Extracts - isolation & purification
Abstract
The house dust mite, Dermatophagoides farinae, was fractionated by a Sephadex G-200 column. Its allergenic (IgE-reacting) and immunogenic (IgG-reacting) components were investigated. By means of skin test, the molecular weight (MW) of major allergenic components of mite was found to be approximately 9,000 to 21,000 daltons. Immunogenic components were investigated by enzyme linked immunosorbent assay using each fraction as an antigen and mice plasma and human serum as antibodies. With mouse plasma, high IgG antibody titers were observed in fractions that contained the part of the mite with high MW (greater than 150,000). With human sera, high IgG antibody titers were observed in fractions that contained the part of the mite with MW more than 30,000. Heterogeneity of human IgG antibody responses against mite antigen was also suggested.
PubMed ID
3484919 View in PubMed
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[Chemical composition and cryoprotective activity of ethanol extract from winter caterpillars Aporia crataegi L.]

https://arctichealth.org/en/permalink/ahliterature9546
Source
Izv Akad Nauk Ser Biol. 2003 Sep-Oct;(5):547-52
Publication Type
Article
Author
N G Li
V L Osakovskii
S S Ivanova
Author Affiliation
Institute of Permafrost Biological Problems, Siberian Division, Russian Academy of Sciences, Leninskii pr. 41, Yakutsk, 677089 Russia.
Source
Izv Akad Nauk Ser Biol. 2003 Sep-Oct;(5):547-52
Language
Russian
Publication Type
Article
Keywords
Animals
Biogenic Amines - isolation & purification - pharmacology
Blood Preservation
Butterflies - chemistry
Carotenoids - isolation & purification - pharmacology
Cell Survival - drug effects - physiology
Crataegus
Cryopreservation
Cryoprotective Agents - isolation & purification - pharmacology
English Abstract
Ethanol - chemistry
Freezing
Glycerol - isolation & purification - pharmacology
Humans
Lymphocytes - drug effects - physiology
Seasons
Tissue Extracts - isolation & purification - pharmacology
Abstract
Glycerol, alpha-carotene, and other yet unidentified compounds have been found in the ethanol extract from winter caterpillars of the black-veined white (Aporia crataegi L.). We have shown that the ethanol extract has a cryoprotective effect on human peripheral blood lymphocytes, and this activity is approximately three times higher than that of glycerol (particularly, in the case of repeated freezing), which is one of the best cryoprotectors.
PubMed ID
14735784 View in PubMed
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