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37 records – page 1 of 4.

Abnormalities within CD4 and CD8 T lymphocytes subsets in type 1 (insulin-dependent) diabetes.

https://arctichealth.org/en/permalink/ahliterature37074
Source
Clin Exp Immunol. 1991 Aug;85(2):278-81
Publication Type
Article
Date
Aug-1991
Author
J. Ilonen
H M Surcel
M L Käär
Author Affiliation
Department of Medical Microbiology, University of Oulu, Finland.
Source
Clin Exp Immunol. 1991 Aug;85(2):278-81
Date
Aug-1991
Language
English
Publication Type
Article
Keywords
Adolescent
Antigens, CD
Antigens, CD4
Antigens, CD45
Antigens, CD8
Antigens, Differentiation, T-Lymphocyte
CD4-Positive T-Lymphocytes - immunology
Child
Diabetes Mellitus, Type 1 - immunology
Female
Histocompatibility Antigens
Humans
Macrophage-1 Antigen
Male
Research Support, Non-U.S. Gov't
T-Lymphocyte Subsets - immunology
Abstract
Abnormalities in the proportions of various T lymphocyte subpopulations have been found in a number of autoimmune diseases. Monoclonal antibodies labelled with various fluorochromes were used here to define the percentages of subsets, and especially to divide CD4+ (helper/inducer) and CD8+ (suppressor/cytotoxic) cells into phenotypic subgroups. Blood samples were analysed from 25 patients (age 10.1 +/- 3.7 years) with recently diagnosed insulin-dependent diabetes mellitus (IDDM) and 25 age- and sex-matched control subjects. The percentages of CD4+ cells and CD4+CD45RA+ cells described as naive T helper cells or suppressor/inducers were increased in the IDDM patients (P less than 0.05 and P less than 0.05. Student's t-test, respectively), whereas the percentage of CD4+CD45RA- cells (memory T-helper cells, helper/inducers) was similar in the patients and controls. The percentage of CD8+CD11b+ cells containing suppressor/effector lymphocytes was decreased in the IDDM patients as compared with the controls (P less than 0.01) but no significant difference was seen in total CD8+ cells. The percentages of CD3+ cells and the proportions of these simultaneously positive for HLA-DR antigen (activated T cells) were also increased in the recent IDDM patients (P less than 0.001 and P less than 0.05, respectively), while the proportion of CD20+ B cells was decreased (P less than 0.05). The findings support the view that disturbed immune regulation occurs in IDDM and indicate that further division of T cell subpopulations may clarify our understanding of the disease process.
PubMed ID
1677834 View in PubMed
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Airway hyperresponsiveness, elevation of serum-specific IgE and activation of T cells following allergen exposure in sensitized Brown-Norway rats.

https://arctichealth.org/en/permalink/ahliterature15906
Source
Immunology. 1995 Aug;85(4):598-603
Publication Type
Article
Date
Aug-1995
Author
A. Haczku
K F Chung
J. Sun
P J Barnes
A B Kay
R. Moqbel
Author Affiliation
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, UK.
Source
Immunology. 1995 Aug;85(4):598-603
Date
Aug-1995
Language
English
Publication Type
Article
Keywords
Allergens - immunology
Animals
Bronchial Hyperreactivity - immunology
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid - immunology
Female
Immunoglobulin E - blood
Lymphocyte Activation - immunology
Ovalbumin - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
T-Lymphocyte Subsets - immunology
Abstract
T lymphocytes may play a regulatory role in the development of allergic airway hyperresponsiveness (AHR). We have studied the relationship between airway responsiveness and a number of immunological changes in Brown-Norway rats sensitized intraperitoneally and repeatedly exposed to ovalbumin (OVA) aerosol. Acetylcholine provocation concentration (PC)150 (the concentration of acetylcholine causing a 150% increase of base-line lung resistance) was measured and peripheral blood and bronchoalveolar lavage (BAL) cells were collected 18-24hr after the final exposure. Total and OVA-specific IgE in serum was measured by enzyme-linked immunosorbent assay (ELISA). Mononuclear cells were analysed by flow cytometry after labelling with monoclonal antibodies against CD2 (pan T-cell marker), CD4, CD8 (T-cell subsets) or CD25 (interleukin-2 receptor). There were significant differences in PC150 (P
PubMed ID
7558155 View in PubMed
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Alefacept: potential new therapy for patients with moderate-to-severe psoriasis.

https://arctichealth.org/en/permalink/ahliterature185907
Source
Issues Emerg Health Technol. 2003 Apr;(45):1-4
Publication Type
Article
Date
Apr-2003
Author
Vijay K Shukla
Source
Issues Emerg Health Technol. 2003 Apr;(45):1-4
Date
Apr-2003
Language
English
Publication Type
Article
Keywords
Canada
Clinical Trials, Phase III as Topic
Drug Approval
Drug Costs
Humans
Psoriasis - drug therapy
Randomized Controlled Trials as Topic
Recombinant Fusion Proteins - adverse effects - economics - therapeutic use
T-Lymphocyte Subsets - immunology
Treatment Outcome
United States
United States Food and Drug Administration
Abstract
Alefacept is a new biotechnology product designed for the treatment of patients with chronic plaque-type psoriasis who have disease severe enough to make them eligible for phototherapy or systemic therapy. In two randomized controlled phase III trials of patients with moderate-to-severe disease, alefacept showed a modest but statistically significant increase in the number of responders compared to placebo. Alefacept's dose-dependent CD4+ T lymphocyte-depleting effect requires monitoring; however, no association has been found between this adverse effect and serious adverse events, particularly infection. Due to lack of direct comparative data, it is difficult to predict exactly how alefacept will fit into the current rotational psoriasis therapy paradigm.
PubMed ID
12680422 View in PubMed
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The effect of immunotherapy on T-cell subsets in peripheral blood and bronchoalveolar lavage fluid in pollen-allergic patients.

https://arctichealth.org/en/permalink/ahliterature16003
Source
Allergy. 1993 Aug;48(6):460-5
Publication Type
Article
Date
Aug-1993
Author
S. Rak
G. Hallden
S. Sörenson
V. Margari
A. Scheynius
Author Affiliation
Department of Lung Medicine, Central Hospital, Västerås, Sweden.
Source
Allergy. 1993 Aug;48(6):460-5
Date
Aug-1993
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Asthma - complications - diagnosis - immunology - therapy
Bronchoalveolar Lavage Fluid
Conjunctivitis, Allergic - complications - diagnosis - immunology - therapy
Desensitization, Immunologic
Female
Flow Cytometry
Forced expiratory volume
HLA-DR Antigens - immunology
Humans
Leukocyte Count
Leukocytes, Mononuclear - immunology
Male
Middle Aged
Pollen
Research Support, Non-U.S. Gov't
Rhinitis, Allergic, Seasonal - complications - diagnosis - immunology - therapy
T-Lymphocyte Subsets - immunology
T-Lymphocytes - immunology
Abstract
The effect of immunotherapy (IT) on T-cell subsets in peripheral blood and bronchoalveolar lavage fluid (BAL) was examined in 15 patients with rhinoconjunctivitis and asthma caused by sensitivity to birch pollen. They were treated with IT for 3 years. Seven patients were treated with highly standardized birch-pollen extract (Pharmacia, Sweden). Eight untreated patients served as controls. Histamine challenge, blood sampling, and BAL were performed before (January, February), and at the peak of, the birch-pollen season (May). The subpopulations of T cells in peripheral blood and BAL fluid were investigated by immunocytochemistry and flow cytometry. During the birch-pollen season, the percentage of CD3+ and CD4+ cells of blood mononuclear cells in the IT patients increased significantly (P
PubMed ID
8238803 View in PubMed
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[Effect of polarized light on the immune status and cytokine levels of patients with bronchial asthma during immunotherapy with bronchomunal]

https://arctichealth.org/en/permalink/ahliterature15354
Source
Fiziol Zh. 2002;48(3):87-94
Publication Type
Article
Date
2002
Author
O V Kravchenko
Author Affiliation
P.L. Shupik Academy of Post-Graduate Education, Ministry of Public Health of Ukraine.
Source
Fiziol Zh. 2002;48(3):87-94
Date
2002
Language
Ukrainian
Publication Type
Article
Keywords
Adjuvants, Immunologic - therapeutic use
Asthma - immunology - physiopathology - therapy
Combined Modality Therapy
Cytokines - blood
English Abstract
Humans
Immunoglobulin E - blood
Immunotherapy
Phototherapy
T-Lymphocyte Subsets - immunology
Treatment Outcome
Abstract
The influence of polarized polychromatic light on immunocompetent cells in complex with immunomodulated bronchomunal is studied. Data of content of the main cytokines taking part in development of inflammation are presented. It is cleared up that polarized light increases the number of T-lymphocyties, normalizes ratio of subpopulation of T-lymphocyties and level of serum FNO-alpha and level of interleukin-4 reaches the level of healthy people. It is ascertained that complex use bronchomunal and polarized polychromatic increases level of serum interferon-gamma.
PubMed ID
12125291 View in PubMed
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The effects of CD8+gammadelta T cells on late allergic airway responses and airway inflammation in rats.

https://arctichealth.org/en/permalink/ahliterature57418
Source
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Publication Type
Article
Date
Sep-2003
Author
Susumu Isogai
Alexandra Rubin
Karim Maghni
David Ramos-Barbon
Rame Taha
Yasuyuki Yoshizawa
Qutayba Hamid
James G Martin
Author Affiliation
Meakins Christie Laboratories, Department of Medicine, McGill University, 3623 St Urbain, Montreal, Quebec, Canada H2X 2P2.
Source
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Date
Sep-2003
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Allergens - administration & dosage
Animals
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis - genetics
Eosinophilia - immunology
Immunoglobulin E - blood
Interferon Type II - biosynthesis - genetics
Interleukin-4 - biosynthesis - genetics
Male
Models, Immunological
Ovalbumin - administration & dosage - immunology
RNA, Messenger - genetics - metabolism
Rats
Rats, Inbred BN
Receptors, Antigen, T-Cell, gamma-delta - metabolism
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - etiology - immunology
T-Lymphocyte Subsets - immunology
Abstract
BACKGROUND: Gamma-delta (gammadelta) T cells regulate immune responses to foreign protein at mucosal surfaces. Whether they can modify allergen-induced early (EAR) and late airway responses (LAR) is unknown. OBJECTIVE: We have tested the hypothesis that the CD8+ subtype of gammadelta T cells decreases allergen-induced LAR and airway eosinophilia in the rat. METHODS: Brown Norway rats were administered, intraperitoneally, 3.5 x 10(4) lymph node CD8+gammadelta T cells from naive or sensitized rats. The recipients were sensitized to ovalbumin (OVA) in Al(OH)(3) 3 days after cell transfer and challenged with aerosolized OVA 14 days later. Serum IgE was measured before allergen challenge. After challenge, lung resistance was monitored for 8 hours and then bronchoalveolar lavage (BAL) was analyzed for eosinophil major basic protein (MBP), IL-4, IL-5, IL-13, and IFN-gamma messenger RNA-expressing cells. RESULTS: gammadelta T cells from naive donors significantly decreased LAR in OVA-challenged sensitized rats, whereas MBP(+) eosinophils were decreased by both gammadelta T cells from naive and sensitized donors. EAR and serum IgE levels were unchanged. The expression of IL-4, IL-5, and IL-13 by BAL cells of gammadelta T cell recipients was attenuated compared with OVA-challenged controls. This was accompanied by an increase in the expression of IFN-gamma. CONCLUSIONS: Our results are consistent with a suppressive role of CD8+gammadelta T cells on allergic airway responses. However, only gammadelta T cells from naive donors inhibit LAR.
PubMed ID
13679814 View in PubMed
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Endogenous and recombinant type I interferons and disease activity in multiple sclerosis.

https://arctichealth.org/en/permalink/ahliterature123419
Source
PLoS One. 2012;7(6):e35927
Publication Type
Article
Date
2012
Author
Finn Sellebjerg
Martin Krakauer
Signe Limborg
Dan Hesse
Henrik Lund
Annika Langkilde
Helle Bach Søndergaard
Per Soelberg Sørensen
Author Affiliation
Danish Multiple Sclerosis Center, Department of Neurology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark. sellebjerg@dadlnet.dk
Source
PLoS One. 2012;7(6):e35927
Date
2012
Language
English
Publication Type
Article
Keywords
Adult
Antigens, CD - immunology
CD4-Positive T-Lymphocytes - immunology
Dendritic Cells
Denmark
Female
Flow Cytometry
HLA-DR Antigens - immunology
Humans
Immunotherapy - methods
Integrin alpha4 - immunology
Interferon Type I - immunology
Interferon-beta - immunology - therapeutic use
Magnetic Resonance Imaging
Male
Middle Aged
Monocytes
Multiple Sclerosis - drug therapy - immunology
Receptors, Transferrin - immunology
Recombinant Proteins - immunology
Statistics, nonparametric
T-Lymphocyte Subsets - immunology
Abstract
Although treatment of multiple sclerosis (MS) with the type I interferon (IFN) IFN-ß lowers disease activity, the role of endogenous type I IFN in MS remains controversial. We studied CD4+ T cells and CD4+ T cell subsets, monocytes and dendritic cells by flow cytometry and analysed the relationship with endogenous type I IFN-like activity, the effect of IFN-ß therapy, and clinical and magnetic resonance imaging (MRI) disease activity in MS patients. Endogenous type I IFN activity was associated with decreased expression of the integrin subunit CD49d (VLA-4) on CD4+CD26(high) T cells (Th1 helper cells), and this effect was associated with less MRI disease activity. IFN-ß therapy reduced CD49d expression on CD4+CD26(high) T cells, and the percentage of CD4+CD26(high) T cells that were CD49d(high) correlated with clinical and MRI disease activity in patients treated with IFN-ß. Treatment with IFN-ß also increased the percentage of CD4+ T cells expressing CD71 and HLA-DR (activated T cells), and this was associated with an increased risk of clinical disease activity. In contrast, induction of CD71 and HLA-DR was not observed in untreated MS patients with evidence of endogenous type IFN I activity. In conclusion, the effects of IFN-ß treatment and endogenous type I IFN activity on VLA-4 expression are similar and associated with control of disease activity. However, immune-activating effects of treatment with IFN-ß may counteract the beneficial effects of treatment and cause an insufficient response to therapy.
Notes
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PubMed ID
22701554 View in PubMed
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Enhanced B cell survival in familial macroglobulinaemia is associated with increased expression of Bcl-2.

https://arctichealth.org/en/permalink/ahliterature69462
Source
Clin Exp Immunol. 1999 Aug;117(2):252-60
Publication Type
Article
Date
Aug-1999
Author
H M Ogmundsdóttir
S. Sveinsdóttir
A. Sigfússon
I. Skaftadóttir
J G Jónasson
B A Agnarsson
Author Affiliation
Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavík. helgam@krabb.is
Source
Clin Exp Immunol. 1999 Aug;117(2):252-60
Date
Aug-1999
Language
English
Publication Type
Article
Keywords
B-Lymphocyte Subsets - immunology - metabolism - pathology
Cell Survival - immunology
Cells, Cultured
Dose-Response Relationship, Immunologic
Flow Cytometry
Humans
Immunoglobulins - biosynthesis
Immunohistochemistry
Lymph Nodes - chemistry - metabolism
Lymphocyte Activation
Pokeweed Mitogens - pharmacology
Proto-Oncogene Proteins c-bcl-2 - biosynthesis - chemistry
Research Support, Non-U.S. Gov't
T-Lymphocyte Subsets - immunology
Time Factors
Waldenstrom Macroglobulinemia - immunology - metabolism - pathology
Abstract
A family with three cases of macroglobulinaemia of undetermined significance (MGUS), and one case each of immunoblastic lymphoma, Waldentr?m's macroglobulinaemia and multiple myeloma was first described 20 years ago. We have previously identified 10 out of 35 healthy family members tested whose lymphocytes produced abnormally high amounts of immunoglobulins in culture. In the present study lymphocyte subpopulations of these hyper-responders have been further characterized and lymphocyte reactivity and survival in vitro have been studied. No differences were detected in the proportions of resting B lymphocytes (CD19+) co-expressing CD5, CD10, CD11b, or CD38, and the CD4/CD8 ratio of T cells was normal before and after stimulation with pokeweed mitogen (PWM). The initial rate of response in terms of immunoglobulin production was not increased, but immunoglobulin levels continued to rise during the second week of culture whereas the production peaked at 8 days in control cultures. This was associated with significantly greater survival of lymphocytes and at 14 days surviving B cells could only be identified in samples from hyper-responders. A lymph node removed because of tuberculosis from a family member 23 years before the diagnosis of multiple myeloma showed very marked Bcl-2 expression in a B cell follicle. This was not seen in a tuberculous lymph node from an unrelated subject. Stimulated cultures from three hyper-responders tested demonstrated significantly higher retention of Bcl-2 in B cells compared with one family control and six unrelated controls. We conclude that the increased production of immunoglobulins previously observed in this family with an inherited tendency for benign and malignant B cell proliferation is the result of enhanced B cell survival, which is associated with increased expression of Bcl-2 following stimulation.
PubMed ID
10444255 View in PubMed
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Enhanced local immune response in children with prolonged gastrointestinal symptoms.

https://arctichealth.org/en/permalink/ahliterature29760
Source
Acta Paediatr. 2004 Dec;93(12):1601-7
Publication Type
Article
Date
Dec-2004
Author
J. Kokkonen
K. Holm
T J Karttunen
M. Mäki
Author Affiliation
Department of Paediatrics, University of Oulu, Oulu, Finland. jorma.kokkonen@ppshp.fi
Source
Acta Paediatr. 2004 Dec;93(12):1601-7
Date
Dec-2004
Language
English
Publication Type
Article
Keywords
Antigens, CD3 - immunology - metabolism
Atrophy - pathology
Celiac Disease - immunology - metabolism - pathology
Child
Duodenoscopy - methods
Duodenum - immunology - metabolism - pathology
Female
Food Hypersensitivity - diet therapy - immunology - metabolism
Gastroscopy - methods
HLA-DR Antigens
Humans
Immunohistochemistry
Intestinal Mucosa - immunology - metabolism
Lymphocyte Count
Male
Receptors, Antigen, T-Cell, alpha-beta - immunology - metabolism
Receptors, Antigen, T-Cell, gamma-delta - immunology - metabolism
T-Lymphocyte Subsets - immunology - metabolism
Abstract
AIM: Recently, we reported typical endoscopic findings and an increment in gammadelta+ T cells in the foregut among children with food-sensitive enteropathy other than coeliac disease. To find out the extend to which the upregulation of the local immune response might explain gastrointestinal (GI) complaints of the foregut, we sought to examine by the increment in gammadelta+ T cells a I-y consecutive series of children referred for recurrent GI complaints to a tertiary-level hospital. METHODS: A 1-y cohort of 102 children scheduled for gastroduodenoscopy were examined for mucosal histology and the densities of CD3+, alphabeta+ and alphabeta+ T-cell subsets from mid-duodenal specimens. The final diagnostic categories were used in analysing the data. RESULTS: Fifteen subjects showed villous atrophy and a high gammadelta+ T-cell density; the finding being compatible with coeliac disease (CD). At the other extreme, 20 subjects in whom diagnostic GI diseases were ruled out showed low densities and served as controls. The subjects reporting GI symptoms after an open food challenge with milk and/or cereals (n = 18) as well as children remitting with a milk- or cereal-eliminating diet but not responding to a challenge (n = 23) also expressed significantly higher densities of gammadelta+ T cells than the controls. In all, 45 of 102 children could be considered to have an elevated gamma6+ T-cell density as an indication of locally activated immune response. Lack of villous architecture and lymphonodular hyperplasia of the duodenal bulb as an endoscopic finding and atopic dermatitis but not the presence of DQ2 alleles showed a close association with these increased densities. CONCLUSION: Considering that an elevated incidence of gammadelta+ T cells is an indication of mucosal response against luminal antigens, up to half the children with prolonged GI symptoms have immune mediated disorder; CD and food allergy being the most obvious clinical entities.
PubMed ID
15841768 View in PubMed
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Evaluation of T cell subsets by an immunocytochemical method compared to flow cytometry in four countries.

https://arctichealth.org/en/permalink/ahliterature7732
Source
Scand J Immunol. 1997 Jun;45(6):637-44
Publication Type
Article
Date
Jun-1997
Author
I M Lisse
B. Böttiger
L B Christensen
K. Knudsen
P. Aaby
A. Gottschau
W. Urassa
F. Mhalu
G. Biberfeld
K. Brattegaard
K. Diallo
P T N'Gom
H. Whittle
Author Affiliation
Department of Pathology, Hvidovre Hospital, Denmark.
Source
Scand J Immunol. 1997 Jun;45(6):637-44
Date
Jun-1997
Language
English
Publication Type
Article
Keywords
Alkaline phosphatase
Antibodies
CD4 Lymphocyte Count - methods
CD4-CD8 Ratio - methods
CD8-Positive T-Lymphocytes - immunology
Comparative Study
Cote d'Ivoire
Denmark
Female
Flow Cytometry - methods - statistics & numerical data
Gambia
Humans
Immunohistochemistry - methods - statistics & numerical data
Male
Reproducibility of Results
Research Support, Non-U.S. Gov't
Sensitivity and specificity
T-Lymphocyte Subsets - immunology
Tanzania
Abstract
The authors tested an alternative method for CD4 and CD8 T lymphocytes enumeration, the immunoalkaline phosphatase method (IA), in three African countries and in Denmark. The IA determinations from 136 HIV antibody positive and 105 HIV antibody negative individuals were compared to the corresponding results obtained by flow cytometry (FC) performed in the respective countries. The authors found good correspondence between the two methods for measurements of CD4 and CD8 T lymphocytes independent of serological status and geographical site. However, the CD4 and CD8 T lymphocytes values obtained by the two methods are not interchangeable as IA compared to FC consistently gives higher percentage of CD4 T lymphocytes, and lower percentage of CD8 T lymphocytes. Mean differences between the two methods did not differ between the three African countries indicating that the IA method provides systematic results. Replicate measurements suggested good correspondence between results obtained by IA. By using an IA level of
PubMed ID
9201303 View in PubMed
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37 records – page 1 of 4.