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22 records – page 1 of 3.

[Activity of enzymes of tricarboxylic and pentose-phosphate cycles in dog brain with myocardial infarction]

https://arctichealth.org/en/permalink/ahliterature56070
Source
Ukr Biokhim Zh. 1977 May-Jun;49(3):51-4
Publication Type
Article
Author
M M Zanozdra
Iu V Khmelevs'kii
Source
Ukr Biokhim Zh. 1977 May-Jun;49(3):51-4
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Brain - enzymology
Cerebellum - enzymology
Cerebral Cortex - enzymology
Citric Acid Cycle
Coronary Vessels
Dogs
English Abstract
Glucosephosphate Dehydrogenase - metabolism
Hypoxia, Brain - enzymology
Ketoglutarate Dehydrogenase Complex - metabolism
Medulla Oblongata - enzymology
Mitochondria - enzymology
Myocardial Infarction - enzymology
Pentosephosphates - metabolism
Succinate Dehydrogenase - metabolism
Transaldolase - metabolism
Abstract
Under conditions of experimental myocardium infarction caused in dogs by ligation of the anterior descending branch of the left coronary artery, the activity of alpha-ketoglutarate dehydrogenase and succinate dehydrogenase in mitochondria of the cortex, cerebellum and medulla ablongata lowers most intensively on the first and fifth day after the appearance of acute myocardium infarction. Activation of the most important enzymes of the pentose-phosphate cycle (glucose-6-phosphate dehydrogenase and transketolase) which is clearly pronounced on the fifth day is observed in the mentioned sections. In the authors' opinions the above changes in the activity of the enzymes are due to the brain hypoxia which may be the main reason of disturbance in the function of the central nervous system under this disease.
PubMed ID
888227 View in PubMed
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Adult-onset calorie restriction delays the accumulation of mitochondrial enzyme abnormalities in aging rat kidney tubular epithelial cells.

https://arctichealth.org/en/permalink/ahliterature78534
Source
Am J Physiol Renal Physiol. 2007 Jun;292(6):F1751-60
Publication Type
Article
Date
Jun-2007
Author
McKiernan Susan H
Tuen Victoria C
Baldwin Katherine
Wanagat Jonathan
Djamali Arjang
Aiken Judd M
Author Affiliation
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI 53706, USA. mckiernan@svm.vetmed.wisc.edu
Source
Am J Physiol Renal Physiol. 2007 Jun;292(6):F1751-60
Date
Jun-2007
Language
English
Publication Type
Article
Keywords
Aging - physiology
Animals
Body Weight - physiology
Caloric Restriction
DNA, Mitochondrial - genetics
Diet
Electron Transport Complex IV - metabolism
Epithelial Cells - enzymology
Gene Deletion
Kidney Tubules - cytology - enzymology
Lasers
Male
Mitochondria - enzymology
Organ Size - physiology
Rats
Rats, Inbred F344
Reverse Transcriptase Polymerase Chain Reaction
Succinate Dehydrogenase - metabolism
Abstract
Adult-onset calorie restriction (A-CR) is an experimental model of life extension and healthy aging less explored, compared with calorie restriction begun at early ages, but one more realistic for human application. We examined the effect of A-CR on the aging rat kidney with respect to common structural age-dependent changes and the accumulation of mitochondrial enzyme abnormalities in tubular epithelial cells. A 40% calorie restriction was initiated in middle-aged rats, before the onset of significant age-related changes in the Fischer x Brown Norway rat kidney. This dietary intervention effectively reduced glomerulosclerosis and tubular atrophy within 6 mo and changed the rate of interstitial fibrosis formation within 1 yr and vascular wall thickening and the expression cytochrome c oxidase (COX)-deficient tubular epithelial cells in 18 mo compared with age-matched ad libitum-fed rats. Our histological approach (histochemical staining for mitochondrial enzyme activity and laser capture microdissection) coupled with mitochondrial DNA (mtDNA) PCR analyses demonstrated that COX-deficient renal tubular epithelial cells accumulated mtDNA deletion mutations and that these cells contained unique, clonally expanded mtDNA deletion mutations. Renal tubular epithelial cells with mitochondrial abnormalities presented cellular characteristics indicative of physiological dysfunction.
PubMed ID
17344189 View in PubMed
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[Antihypoxic effect of vitamin E and its derivative in rats under modeling of hypoxic conditions of different origin]

https://arctichealth.org/en/permalink/ahliterature9607
Source
Ukr Biokhim Zh. 2003 Mar-Apr;75(2):67-71
Publication Type
Article
Author
F R Zvaid
G V Donchenko
I V Kuz'menko
Author Affiliation
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Source
Ukr Biokhim Zh. 2003 Mar-Apr;75(2):67-71
Language
Russian
Publication Type
Article
Keywords
Animals
Anoxia - metabolism
Electron Transport
English Abstract
Enzyme Stability
Epinephrine - pharmacology
Leukocytes - enzymology - metabolism
Mitochondria, Heart - enzymology - metabolism
Myocarditis - chemically induced - metabolism
NAD(P)H Dehydrogenase (Quinone) - metabolism
Rats
Rats, Wistar
Succinate Dehydrogenase - metabolism
Ubiquinone - drug effects - metabolism
Vitamin E - pharmacology
Abstract
The increase of ubiquinone content in myocardial mitochondria and succinateubiquinone reductase activity in rat blood leucocytes under hypoxic hypoxia and acute microfocal myocardial damage [table: see text] have been shown. At the same time the succinateubiquinone reductase activity of rat myocardial mitochondria do not change substantially. We simultaneously observe the dramatic drop in NADH-ubiquinone reductase activity under experimental myocarditis. This fact demonstrates higher stability of succinateubiquinone reductase system and differences in metabolical processes under hypoxic conditions of different origin. All vitamin E derivatives studied demonstrate substantial antihypoxic activity, which is connected with increased animals' survivability, ubiquinone content in myocardial mitochondria and stabilization and leveling of succinateubiquinone reducatse activity in rat blood leucocytes. alpha-Tocopherolacetate with shortened to six carbon atoms side chain is the most effective in this respect. We discuss possible mechanisms for the realization of vitamin E and its active derivative's antihypoxic effect.
PubMed ID
14577173 View in PubMed
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Chronic alcohol intake induces the oxidative capacity of brown adipose tissue in the rat.

https://arctichealth.org/en/permalink/ahliterature12535
Source
Pharmacol Biochem Behav. 1988 Jan;29(1):53-7
Publication Type
Article
Date
Jan-1988
Author
P. Huttunen
M L Kortelainen
Author Affiliation
Department of Forensic Medicine, University of Oulu, Finland.
Source
Pharmacol Biochem Behav. 1988 Jan;29(1):53-7
Date
Jan-1988
Language
English
Publication Type
Article
Keywords
Alcoholism - metabolism - pathology
Animals
Body Weight - drug effects
Brown Fat - drug effects - metabolism - pathology
Cold
Electron Transport Complex IV - metabolism
Ethanol - toxicity
Male
Myocardium - enzymology - pathology
Organ Size - drug effects
Oxidation-Reduction
Proteins - metabolism
Rats
Rats, Inbred Strains
Succinate Dehydrogenase - metabolism
Abstract
The present study was carried out to elucidate the effect of long-term alcohol intake on the oxidative capacity of brown adipose tissue in the rat. Rats housed at room temperature were given water containing 10% ethanol for six months, while controls received water alone. Fully cold-acclimated rats (exposed to +4 degrees C for 6 weeks) served as the second control group. Alcohol did not alter the food intake of the rats compared with the controls kept at room temperature, but it did cause a mean decrease of 8 ml in fluid consumption. There was no difference in the increase in body weight between the groups housed at room temperature. Body weight of the rats exposed to cold did not change during cold acclimation. No morphological liver changes were observed in alcohol-fed rats, but some changes related to long-term alcohol consumption were found in the myocardium. Chronic alcohol intake increased the quantity of brown adipose tissue and its protein content but changes were not as great as in the cold-acclimated rats nor did alcohol increase protein content per unit of the adipose tissue as did cold. On the other hand, the specific activity of mitochondrial cytochrome oxidase increased by 90% and that of succinate dehydrogenase by 130% in alcohol-fed rats, whereas specific activities of these enzymes displayed little or no change in the cold-acclimated rats. Results suggest that chronic alcohol ingestion induces the oxidative capacity of the interscapular brown adipose tissue in the rat, increasing the mass of BAT and specific activities of mitochondrial enzymes.
PubMed ID
2832855 View in PubMed
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[Correction of the activity of certain enzymes in the rat liver mitochondrial electron transport chain by derivatives of alpha-tocopheryl acetate in toxic damage to the liver]

https://arctichealth.org/en/permalink/ahliterature56679
Source
Ukr Biokhim Zh. 1999 Jul-Aug;71(4):30-4
Publication Type
Article
Author
V N Kovalenko
I S Blazhchuk
A M Shaiakhmetova
I V Kuz'menko
Author Affiliation
Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine, Kyiv.
Source
Ukr Biokhim Zh. 1999 Jul-Aug;71(4):30-4
Language
Russian
Publication Type
Article
Keywords
Animals
Carbon Tetrachloride - toxicity
Electron Transport
English Abstract
Enzyme Activation
Hepatitis, Toxic - enzymology - metabolism
Male
Mitochondria, Liver - drug effects - enzymology
NADH Dehydrogenase - metabolism
Rats
Succinate Cytochrome c Oxidoreductase - metabolism
Succinate Dehydrogenase - metabolism
Vitamin E - analogs & derivatives - chemistry - pharmacology
alpha-Tocopherol - analogs & derivatives
Abstract
Correcting action of vitamin E and it's short chain derivative on the activity of some mitochondria electron transport chain enzymes were investigated on models of acute and chronic toxic hepatitis. Inhibition of NADH- and succinate-cytochrome c oxidoreductase complexes activity was established in short term action of xenobiotics. Treatment of rats with CCl4 during 60 days lowered activity of NADH-cytochrome c oxidoreductase complex and significantly increased activity of succinate-cytochrome c oxidoreductase complex and succinate dehydrogenase. Obviously, as a result of long term influence of hepatotoxic agents switching over in rat mitochondria electron transport from NAD-dependent way of substrate oxidation to succinate-dependent way took place. This event could be a part of the body adaptation mechanisms. Vitamin E and its short chain analogue corrected activities of investigated enzymes of mitochondria liver in the animals with acute and chronic hepatitis.
PubMed ID
10791053 View in PubMed
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Downregulation in muscle Na(+)-K(+)-ATPase following a 21-day expedition to 6,194 m.

https://arctichealth.org/en/permalink/ahliterature6773
Source
J Appl Physiol. 2000 Feb;88(2):634-40
Publication Type
Article
Date
Feb-2000
Author
H. Green
B. Roy
S. Grant
M. Burnett
R. Tupling
C. Otto
A. Pipe
D. McKenzie
Author Affiliation
Department of Kinesiology, University of Waterloo, Waterloo N2L3G1, Ontario K1Y4E9.
Source
J Appl Physiol. 2000 Feb;88(2):634-40
Date
Feb-2000
Language
English
Publication Type
Article
Keywords
3-Hydroxyacyl CoA Dehydrogenases - metabolism
Acclimatization - physiology
Adult
Altitude
Body Weight
Citrate (si)-Synthase - metabolism
Down-Regulation
Female
Heart Rate - physiology
Humans
L-Lactate Dehydrogenase - metabolism
Malate Dehydrogenase - metabolism
Male
Mountaineering
Muscle, Skeletal - enzymology
Na(+)-K(+)-Exchanging ATPase - metabolism
Oxygen Consumption - physiology
Pulmonary Ventilation - physiology
Research Support, Non-U.S. Gov't
Succinate Dehydrogenase - metabolism
Time Factors
Abstract
To investigate the hypothesis that acclimatization to altitude would result in a downregulation in muscle Na(+)-K(+)-ATPase pump concentration, tissue samples were obtained from the vastus lateralis muscle of six volunteers (5 males and 1 female), ranging in age from 24 to 35 yr, both before and within 3 days after a 21-day expedition to the summit of Mount Denali, Alaska (6,194 m). Na(+)-K(+)-ATPase, measured by the [(3)H]ouabain-binding technique, decreased by 13.8% [348 +/- 12 vs. 300 +/- 7.6 (SE) pmol/g wet wt; P
PubMed ID
10658031 View in PubMed
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Effect of benzo[a]pyrene on the immune status of mice with anxious-depressive syndrome.

https://arctichealth.org/en/permalink/ahliterature83139
Source
Bull Exp Biol Med. 2005 Jul;140(1):70-3
Publication Type
Article
Date
Jul-2005
Author
Verbitskaya L V
Tenditnik M V
Michurina S V
Shurlygina A V
Trufakin V A
Author Affiliation
Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk.
Source
Bull Exp Biol Med. 2005 Jul;140(1):70-3
Date
Jul-2005
Language
English
Publication Type
Article
Keywords
Animals
Anxiety Disorders - immunology
Benzo(a)pyrene - toxicity
Blood Cell Count
Depressive Disorder - immunology
Energy Metabolism - drug effects
Glycerolphosphate Dehydrogenase - metabolism
Immunity - drug effects
Immunohistochemistry
L-Lactate Dehydrogenase - metabolism
Lymphocytes - drug effects
Male
Mice
Mice, Inbred C57BL
Statistics, nonparametric
Succinate Dehydrogenase - metabolism
Abstract
We studied the effect of benzo[a]pyrene on cells of lymphoid organs, energy metabolism of blood lymphocytes, and immunological reactivity in mice with anxious depressive syndrome produced by social stress. Benzo[a]pyrene exhibited a more pronounced immunotoxic activity in anxious-depressive animals, which was more than a simple sum of the effects produced by adverse ecological and psychic factors.
PubMed ID
16254624 View in PubMed
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[Energetic aspects of adaptation processes and rehabilitation].

https://arctichealth.org/en/permalink/ahliterature180014
Source
Vopr Kurortol Fizioter Lech Fiz Kult. 2004 Mar-Apr;(2):30-2
Publication Type
Article
Author
M P Tovbushenko
Source
Vopr Kurortol Fizioter Lech Fiz Kult. 2004 Mar-Apr;(2):30-2
Language
Russian
Publication Type
Article
Keywords
Adaptation, physiological - physiology
Adenine Nucleotides - metabolism
Balneology - methods
Blood Glucose - analysis
Duodenal Ulcer - blood - metabolism - physiopathology - rehabilitation
Energy Metabolism
Erythrocytes - metabolism
Glycerolphosphate Dehydrogenase - metabolism
Health Resorts
Humans
Lipid Peroxides - blood
Russia
Succinate Dehydrogenase - metabolism
Abstract
The study of energy metabolism and phenotypical adaptation in patients with ulcer disease has revealed deficiency of energy metabolism and defective phenotypical adaptation in the form of its tension or failure of adaptive processes. Sanatorium treatment with oral intake of mineral water, mineral baths, fangotherapy, limontar-electrophoresis and millimetric waves promoted normalization of energy metabolism and adaptive processes. Impact of different physiotherapeutic factors on energy metabolism and adaptation of the organism is described.
PubMed ID
15154352 View in PubMed
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Exobiology and the effect of physical factors on micro-organisms.

https://arctichealth.org/en/permalink/ahliterature13595
Source
Life Sci Space Res. 1967;5:250-60
Publication Type
Article
Date
1967
Author
A A Imshenetsky
S S Abyzov
G T Voronov
L A Kuzjurina
S V Lysenko
G G Sotnikov
R I Fedorova
Author Affiliation
Institute of Microbiology, Academy of Sciences of the USSR, Moscow, USSR.
Source
Life Sci Space Res. 1967;5:250-60
Date
1967
Language
English
Publication Type
Article
Keywords
Alcohol Dehydrogenase - metabolism
Azotobacter vinelandii - enzymology - metabolism
Bacillus cereus - growth & development - radiation effects
Bacillus megaterium - growth & development - radiation effects
Catalase - metabolism
Chromium
Exobiology
Extraterrestrial Environment
Mars
Meteoroids
Peroxidases - metabolism
Radiation Protection
Soil Microbiology
Spores, Bacterial - growth & development - radiation effects
Succinate Dehydrogenase - metabolism
Temperature
Ultraviolet Rays
Vacuum
Abstract
A study of the action of different physical factors on micro-organisms is necessary for a further development of exobiology. The action of temperature on crystalline preparations of catalase and peroxidase was studied by means of oscillographic polarography. A determination of the height of polarographic waves at the decrease of temperature from 20 degrees C to 0 degrees C has shown that structural elements of the peroxidase molecule connected with the enzymatic activity are more stable with the decrease of temperature cf. catalase. A relative resistance of the dehydrogenase activity in Az. vinelandii cells to high vacuum was found. Incubation of azotobacter cells under vacuum of 10(-9) mm Hg during 72 hr did not decrease the activity of alcohol and succinic dehydrogenase. Bac. cereus spores can be preserved from bactericidal UV action by thin films of chrome. The thickness of chrome film being 200-670 angstroms, spores are killed by a dose of 7.8 x 10(7) erg/cm2 at 253.7 microns wave length. Spores covered by chrome film thicker than 800 angstroms remain alive after this treatment. Investigations carried out with an 'Artificial Mars' camera led to the following results. The growth of Bac. megaterium on liquid growth media in this camera ceases as a result of UV rays killing all cells after 3 weeks. Untreated bacteria grow in the camera for a long time. Spore-forming bacteria isolated from the sand of the Kara-Kum Desert grow in ground limonite (with the addition of 2% garden soil) having maximum hygroscopic humidity (3.8%). Freezing and thawing (from -60 degrees C to +25 degrees C) corresponding to day temperature deviations on Mars, low pressure (P=10 mm Hg) and the composition of the atmosphere (CO2-50%, N2-40%, Ar-10%) do not influence the growth of xerophylic bacteria under study. Humidity is the main factor limiting the growth of micro-organisms under 'Artificial Mars' conditions. According to the further development of the microbiological meteorite analysis methods, samples of rocks and stone meteorites were sterilized, incubated in the desert or on a snow surface in the Arctic and after different times (from 100 days to 7 months), investigated. In all cases, microbes were found only on the sample surfaces, whereas 1 cm from the surface and in the central parts micro-organism were completely absent. Hence, microbiological analysis of central parts of meteorites fallen in the Arctic or during dry periods of the year in the desert can give reliable results.
PubMed ID
11973848 View in PubMed
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22 records – page 1 of 3.