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3423 records – page 1 of 343.

A 1-year evaluation of Syva MicroTrak Chlamydia enzyme immunoassay with selective confirmation by direct fluorescent-antibody assay in a high-volume laboratory.

https://arctichealth.org/en/permalink/ahliterature217461
Source
J Clin Microbiol. 1994 Sep;32(9):2208-11
Publication Type
Article
Date
Sep-1994
Author
E L Chan
K. Brandt
G B Horsman
Author Affiliation
Laboratory and Disease Control Services, Saskatchewan Health, Regina, Canada.
Source
J Clin Microbiol. 1994 Sep;32(9):2208-11
Date
Sep-1994
Language
English
Publication Type
Article
Keywords
Algorithms
Chlamydia Infections - diagnosis - epidemiology - microbiology
Chlamydia trachomatis - immunology - isolation & purification
Cost Control
Densitometry
Diagnostic Tests, Routine - economics
Evaluation Studies as Topic
Female
Fluorescent Antibody Technique - economics
Humans
Immunoenzyme Techniques - economics
Male
Predictive value of tests
Prevalence
Reagent kits, diagnostic
Saskatchewan - epidemiology
Seasons
Sensitivity and specificity
Urethritis - diagnosis - epidemiology - microbiology
Uterine Cervicitis - diagnosis - epidemiology - microbiology
Abstract
TThe Syva MicroTrak Chlamydia enzyme immunoassay (EIA; Syva Company, San Jose, Calif.) with cytospin and direct fluorescent-antibody assay (DFA) confirmation was evaluated on 43,630 urogenital specimens over a 1-year period in the Provincial Laboratory in Regina, Saskatchewan, Canada. This was a two-phase study intended to define a testing algorithm for Chlamydia trachomatis that would be both highly accurate and cost-effective in our high-volume (> 3,000 tests per month) laboratory. The prevalence of C. trachomatis infection in our population is moderate (8 to 9%). In phase 1, we tested 6,022 male and female urogenital specimens by EIA. All specimens with optical densities above the cutoff value and those within 30% below the cutoff value were retested by DFA. This was 648 specimens (10.8% of the total). A total of 100% (211 of 211) of the specimens with optical densities equal to or greater than 1.00 absorbance unit (AU) above the cutoff value, 98.2% (175 of 178) of the specimens with optical densities of between 0.500 and 0.999 AU above the cutoff value, and 83% (167 of 201) of the specimens with optical densities within 0.499 AU above the cutoff value were confirmed to be positive. A total of 12% (7 of 58) of the specimens with optical densities within 30% below the cutoff value were positive by DFA. In phase 2, we tested 37,608 specimens (32,495 from females; 5,113 from males) by EIA. Only those specimens with optical densities of between 0.499 AU above and 30% below the cutoff value required confirmation on the basis of data from phase 1 of the study. This was 4.5% of all specimens tested. This decrease in the proportion of specimens requiring confirmation provides a significant cost savings to the laboratory. The testing algorithm gives us a 1-day turnaround time to the final confirmed test results. The MicroTrak EIA performed very well in both phases of the study, with a sensitivity, specificity, positive predictive value, and negative predictive value of 96.1, 99.1, 90.3, and 99.7%, respectively, in phase 2. We suggest that for laboratories that use EIA for Chlamydia testing, a study such as this one will identify an appropriate optical density range for confirmatory testing for samples from that particular population.
Notes
Cites: Epidemiol Rev. 1983;5:96-1236357824
Cites: J Clin Microbiol. 1993 Jun;31(6):1646-78315010
Cites: Diagn Microbiol Infect Dis. 1992 Nov-Dec;15(8):663-81478048
Cites: J Clin Microbiol. 1990 Nov;28(11):2473-62254422
PubMed ID
7814548 View in PubMed
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A 2-dose regimen of a recombinant hepatitis B vaccine with the immune stimulant AS04 compared with the standard 3-dose regimen of Engerix-B in healthy young adults.

https://arctichealth.org/en/permalink/ahliterature56626
Source
Scand J Infect Dis. 2002;34(8):610-4
Publication Type
Article
Date
2002
Author
K. Levie
I. Gjorup
P. Skinhøj
M. Stoffel
Source
Scand J Infect Dis. 2002;34(8):610-4
Date
2002
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Belgium
Comparative Study
Denmark
Dose-Response Relationship, Drug
Female
Hepatitis B - prevention & control
Hepatitis B Antibodies - analysis
Hepatitis B Surface Antigens - analysis
Hepatitis B vaccines - administration & dosage
Humans
Immunity - physiology
Immunization - methods
Immunization Schedule
Male
Reference Values
Research Support, Non-U.S. Gov't
Sensitivity and specificity
Single-Blind Method
Vaccines, Synthetic - administration & dosage
Abstract
An open-label randomized study was undertaken to compare a 2-dose regimen (Months 0 and 6) of hepatitis B surface antigen (HBsAg) vaccine formulated with a novel adjuvant (HBsAg/AS04) with a standard 3-dose regimen (Months 0, 1 and 6) of licensed recombinant HBsAg vaccine in terms of immunogenicity and reactogenicity when administered to healthy subjects aged between 15 and 40 y. At 1 and 6 months after the full vaccination course there was a 100% seroprotection rate (anti-HBs > or = 10 mIU/ml) with the HBsAg/AS04 vaccine, compared with a 99% response rate with the licensed vaccine. The corresponding geometric mean titres were significantly higher for the novel vaccine compared to the standard vaccine: 15,468 and 2,745 mIU/ml at Months 7 and 12 vs. 6,274 and 1,883 mIU/ml, respectively. There was a higher prevalence of local symptoms with the adjuvant vaccine (90% of doses) than with the standard vaccine (48% of doses). However, these symptoms (pain, swelling and redness) were predominantly of mild-to-moderate intensity and resolved rapidly without treatment. A 2-dose regimen of the new HBsAg/AS04 adjuvant vaccine therefore compared favourably to the standard regimen in healthy young adults. It is anticipated that the simplified vaccination schedule may improve compliance and reduce costs.
PubMed ID
12238579 View in PubMed
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(3)H activity comparison between FTMC, VNIIM and LNE-LNHB.

https://arctichealth.org/en/permalink/ahliterature278451
Source
Appl Radiat Isot. 2016 Mar;109:41-3
Publication Type
Article
Date
Mar-2016
Author
Philippe Cassette
Paulius Butkus
Arunas Gudelis
Tatiana Shilnikova
Source
Appl Radiat Isot. 2016 Mar;109:41-3
Date
Mar-2016
Language
English
Publication Type
Article
Keywords
France
Internationality
Laboratories - standards
Lithuania
Nuclear Medicine - standards
Radiation Dosage
Radiometry - standards
Reference Values
Reproducibility of Results
Russia
Scintillation Counting
Sensitivity and specificity
Tritium - analysis - standards
Abstract
An activity comparison of tritiated water was organized in 2013 between 3 laboratories: FTMC (Lithuania), LNE-LNHB (France) and VNIIM (Russia). The solution was prepared by LNHB and ampoules were sent to the others laboratories. This solution was standardized in terms of activity per unit mass by participant laboratories using the Triple to Double Coincidence Ratio (TDCR) method in liquid scintillation counting (LSC). The tritiated water solution is traceable to the solution prepared by LNHB for the CCRI(II)-K2.H-3 2009 (3)H international comparison.
PubMed ID
26651170 View in PubMed
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The 3 mm skin prick test (SPT) threshold criterion is not reliable for Tyrophagus putrescentiae: the re-evaluation of SPT criterion to dust mites.

https://arctichealth.org/en/permalink/ahliterature71486
Source
Allergy. 2002 Dec;57(12):1187-90
Publication Type
Article
Date
Dec-2002
Author
B. Kanceljak-Macan
J. Macan
D. Plavec
T. Klepac
S. Milkovic-Kraus
Author Affiliation
Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Source
Allergy. 2002 Dec;57(12):1187-90
Date
Dec-2002
Language
English
Publication Type
Article
Keywords
Adult
Antibody Specificity - immunology
Comparative Study
Croatia
Cross Reactions - immunology
Dermatophagoides farinae - immunology
Dermatophagoides pteronyssinus - immunology
Female
Humans
Immunoglobulin E - blood - immunology
Male
Middle Aged
Proteins - immunology
Pyroglyphidae - immunology
Regression Analysis
Reproducibility of Results
Sensitivity and specificity
Skin Tests - standards
Urban health
Abstract
BACKGROUND: The mean wheal diameter >/= 3 mm is the usual criterion for positive skin prick test (SPT) reaction to dust mites. The study assessed the accuracy of this SPT criterion with respect to specific IgE values of above 0.35 kUA/l (+ sIgE). METHODS: Specific IgE (ImmunoCAP, Pharmacia AB Diagnostics, Uppsala, Sweden) and standard SPT to Dermatophagoides pteronyssinus (DP) and farinae (DF), Lepidoglyphus destructor (LD) and Tyrophagus putrescentiae (TP) (ALK, Hørsholm, Denmark) were performed in a random sample of 457 subjects, of whom 273 men (mean age 35.3 +/- 11.0 years) and 184 women (mean age 37.9 +/- 9.5 years). Statistical analysis was performed using the chi-square test, regression analysis and discriminant analysis. RESULTS: When the mean wheal diameter of >/= 3 mm was considered positive (+ SPT), the correlation between + SPT and + sIgE was 0.47 for DP (P
PubMed ID
12464048 View in PubMed
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A 7-day activity diary for assessment of daily energy expenditure validated by the doubly labelled water method in adolescents.

https://arctichealth.org/en/permalink/ahliterature52682
Source
Eur J Clin Nutr. 1997 Sep;51(9):585-91
Publication Type
Article
Date
Sep-1997
Author
L E Bratteby
B. Sandhagen
H. Fan
G. Samuelson
Author Affiliation
Department of Clinical Physiology, University Hospital, Uppsala, Sweden.
Source
Eur J Clin Nutr. 1997 Sep;51(9):585-91
Date
Sep-1997
Language
English
Publication Type
Article
Keywords
Activities of Daily Living
Adolescent
Basal Metabolism
Calorimetry, Indirect
Comparative Study
Deuterium - diagnostic use
Energy Metabolism
Exertion
Female
Humans
Male
Oxygen Isotopes
Research Support, Non-U.S. Gov't
Sensitivity and specificity
Sweden
Water
Abstract
OBJECTIVES: To validate the use of an activity diary and predicted BMR for assessment of daily total energy expenditure (TEE) and physical activity level (PAL = TEE/BMR) in adolescents. DESIGN: TEE and PAL estimated from activity diary records kept for seven days and BMR predicted from age, gender and body weight were compared with the results of doubly labelled water (DLW) measurements and indirect calorimetry performed during the same time period. SETTING: The Unit of paediatric Physiology of the Department of Clinical Physiology, University Hospital, Uppsala, Sweden. SUBJECTS: Fifty randomly selected 15 y old adolescents (25 boys and 25 girls). RESULTS: The mean difference between TEE estimated in all adolescents by the activity diary and by DLW methods was 1.2%. The limits of agreement (mean difference 2 s.d.) were -3.47 and 3.77 MD/d, equivalent to a coefficient of variation of 15%. The mean difference between PAL assessed by activity diary records and by DLW measurements was 0.001, and the limits of agreement between the two methods were 0.54. CONCLUSIONS: The results imply that the activity diary method provides a close estimate of TEE and PAL in population groups.
PubMed ID
9306084 View in PubMed
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16S rDNA sequencing of valve tissue improves microbiological diagnosis in surgically treated patients with infective endocarditis.

https://arctichealth.org/en/permalink/ahliterature134307
Source
J Infect. 2011 Jun;62(6):472-8
Publication Type
Article
Date
Jun-2011
Author
Martin Vondracek
Ulrik Sartipy
Ewa Aufwerber
Inger Julander
Dan Lindblom
Katarina Westling
Author Affiliation
Department of Clinical Microbiology, Karolinska University Hospital and Department of Clinical Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Source
J Infect. 2011 Jun;62(6):472-8
Date
Jun-2011
Language
English
Publication Type
Article
Keywords
Adult
Aged
Bacteria - classification - genetics - isolation & purification
Bacteriological Techniques - methods
DNA, Bacterial - chemistry - genetics
DNA, Ribosomal - chemistry - genetics
Endocarditis - diagnosis - microbiology - surgery
Female
Heart Valves - microbiology
Humans
Male
Middle Aged
RNA, Ribosomal, 16S - genetics
Sensitivity and specificity
Sequence Analysis, DNA - methods
Sweden
Abstract
The aim was to evaluate 16S rDNA sequencing in heart valves in patients with infective endocarditis undergoing surgery.
Fifty-seven patients with infective endocarditis were examined in this prospective study by analysing heart valves with 16S rDNA sequencing and culturing methods and comparing the results to blood cultures. As controls, heart valves from 61 patients without any signs of endocarditis were examined.
All together 77% of the endocarditis patients were positive for 16S rDNA, 84% had positive blood cultures and 23% had positive cultures from heart valves, whereas only 16% of the cultures from heart valves were concordant with results from blood cultures or 16S rDNA. Concordant results between 16S rDNA sequencing and blood cultures were found in 75% patients. All controls were negative for 16S rDNA. In 4 out of 9 patients with negative blood cultures, the aetiology was established by 16S rDNA alone, i.e. viridans group streptococci.
In this Swedish study, 16S rDNA sequencing of valve material was shown to be a valuable addition in blood culture-negative cases. The value of heart valve culture was low. Molecular diagnosis using 16S rDNA sequencing should be recommended in patients undergoing valve replacement for infective endocarditis.
PubMed ID
21601285 View in PubMed
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18F-fluorodeoxyglucose positron emission tomography-computed tomography for suspected recurrent papillary thyroid cancer: early experience at Sunnybrook Health Sciences Centre.

https://arctichealth.org/en/permalink/ahliterature153281
Source
J Otolaryngol Head Neck Surg. 2008 Oct;37(5):712-7
Publication Type
Article
Date
Oct-2008
Author
Max Dahele
Yee C Ung
Lisa Ehrlich
Jay Silverberg
Judith Balogh
C Shun Wong
Author Affiliation
Departmentof Radiation Oncology, University of Toronto, Edmond Odette Cancer Centre,Toronto, Ontario.
Source
J Otolaryngol Head Neck Surg. 2008 Oct;37(5):712-7
Date
Oct-2008
Language
English
Publication Type
Article
Keywords
Adult
Aged
Biological Markers - blood
Cancer Care Facilities
Carcinoma, Papillary - pathology - radionuclide imaging - surgery
Cohort Studies
Female
Fluorodeoxyglucose F18 - diagnostic use
Follow-Up Studies
Humans
Male
Middle Aged
Neoplasm Recurrence, Local - pathology - radionuclide imaging - surgery
Neoplasm Staging
Ontario
Positron-Emission Tomography - methods
Reproducibility of Results
Retrospective Studies
Risk assessment
Sensitivity and specificity
Thyroglobulin - blood
Thyroid Neoplasms - pathology - radionuclide imaging - surgery
Thyroidectomy - methods
Time Factors
Treatment Outcome
Young Adult
Abstract
To report the initial experience with combined 18F-fluorodeoxyglucose positron emission tomography (FDG PET)/computed tomography (CT) imaging for suspected recurrent papillary differentiated thyroid cancer (DTC) at Sunnybrook Health Sciences Centre (SHSC), Toronto.
Single institution retrospective study.
Consecutive patients from SHSC who underwent FDG PET/CT imaging for suspected recurrent DTC over a period of 2.5 years were identified and their charts reviewed.
Qualitative appraisal of FDG PET/CT imaging in suspected recurrent DTC.
Sixteen patients (14F, 2M) were identified accounting for 17 FDG PET/CT scans. Three scans (18%) in 3 different patients were reported as suspicious for recurrent disease in the neck (1-3 lesions) and were considered "positive". All were subsequently confirmed pathologically (4-13 positive lymph nodes post operatively). Prior conventional imaging was abnormal in two patients. Two patients had an elevated non-stimulated thyroglobulin (TG)
PubMed ID
19128681 View in PubMed
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20 Years of standard patch testing in an eczema population with focus on patients with multiple contact allergies.

https://arctichealth.org/en/permalink/ahliterature162478
Source
Contact Dermatitis. 2007 Aug;57(2):76-83
Publication Type
Article
Date
Aug-2007
Author
Berit Christina Carlsen
Torkil Menné
Jeanne Duus Johansen
Author Affiliation
National Allergy Research Centre, Department of Dermatology, Copenhagen University Hospital Gentofte, 2820 Gentofte, Denmark. bccarlsen@dadlnet.dk
Source
Contact Dermatitis. 2007 Aug;57(2):76-83
Date
Aug-2007
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Age Distribution
Aged
Allergens - diagnostic use
Child
Databases, Factual
Denmark - epidemiology
Dermatitis, Allergic Contact - complications - diagnosis - epidemiology
Eczema - complications
Female
Humans
Male
Middle Aged
Patch Tests - methods
Predictive value of tests
Retrospective Studies
Sensitivity and specificity
Sex Distribution
Sex Factors
Abstract
Results of standard patch tests performed with the same methodology in one centre are rarely available over a large time span. This gives the unique opportunity to study not only prevalence but also persistency of contact allergy and characterize subpopulations. The objectives were to investigate sensitivity rates and persistencies of patch test results and characterize patients with multiple contact allergies. A 20-year retrospective database-based study of 14 998 patients patch tested with the European Standard Series was performed. 34.5% were sensitized, primarily women. Sensitivity to nickel was most frequent and least frequent to mercaptobenzothiazole, N-isopropyl-N-phenyl-p-phenylenediamine and benzocaine. Yearly proportion of negative, mono/double-allergic, and multiple-allergic cases remained stable. Persistency of positive reactions was high for para-phenylenediamine, Cl(Me)isothiazolinone, and primin and poor for paraben mix. 5.1% were multiple allergic, primarily women, and 90% got diagnosed by the first test. Frequency of multiple allergies increased with age. More multiple- than mono/double-allergic patients were tested multiple times. Persistency and sensitivity rates in a Danish eczema population are provided and are useful for decisions regarding the standard series. Patients with multiple contact allergies are typically elderly women who might have long-lasting and hard-to-treat eczema. Cumulative environmental exposure seems necessary to develop multiple allergies.
PubMed ID
17627644 View in PubMed
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The 24-hour urine collection: gold standard or historical practice?

https://arctichealth.org/en/permalink/ahliterature155561
Source
Am J Obstet Gynecol. 2008 Dec;199(6):625.e1-6
Publication Type
Article
Date
Dec-2008
Author
Anne-Marie Côté
Tabassum Firoz
André Mattman
Elaine M Lam
Peter von Dadelszen
Laura A Magee
Author Affiliation
Department of Nephrology, University of Sherbrooke, Sherbrooke, PQ, Canada.
Source
Am J Obstet Gynecol. 2008 Dec;199(6):625.e1-6
Date
Dec-2008
Language
English
Publication Type
Article
Keywords
Adult
Biological Markers - urine
British Columbia
Cohort Studies
Creatinine - urine
Female
Gynecology - standards
Hospitals, University
Humans
Hypertension - diagnosis - urine
Pre-Eclampsia - diagnosis - urine
Pregnancy
Pregnancy Complications, Cardiovascular - diagnosis - urine
Pregnancy outcome
Prenatal Care - standards
Reference Standards
Retrospective Studies
Sensitivity and specificity
Time Factors
Urinalysis - standards
Young Adult
Abstract
The objective of the study was to determine completeness of 24-hour urine collection in pregnancy.
This was a retrospective laboratory/chart review of 24-hour urine collections at British Columbia Women's Hospital. Completeness was assessed by 24-hour urinary creatinine excretion (UcreatV): expected according to maternal weight for single collections and between-measurement difference for serial collections.
For 198 randomly selected pregnant women with a hypertensive disorder (63% preeclampsia), 24-hour urine collections were frequently inaccurate (13-54%) on the basis of UcreatV of 97-220 micromol/kg per day (11.0-25.0 mg/kg per day) or 133-177 micromol/kg per day (15.1-20.1 mg/kg per day) of prepregnancy weight (respectively). Lean body weight resulted in more inaccurate collections (24-68%). The current weight was frequently unavailable (28%) and thus not used. For 161 women (81% proteinuric) with serial 24-hour urine levels, a median [interquartile range] of 11 [5-31] days apart, between-measurement difference in UcreatV was 14.4% [6.0-24.9]; 40 women (24.8%) had values 25% or greater, exceeding analytic and biologic variation.
Twenty-four hour urine collection is frequently inaccurate and not a precise measure of proteinuria or creatinine clearance.
PubMed ID
18718568 View in PubMed
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A 25-year follow-up of a population screened with faecal occult blood test in Finland.

https://arctichealth.org/en/permalink/ahliterature161415
Source
Acta Oncol. 2007;46(8):1103-6
Publication Type
Article
Date
2007
Author
Nea Malila
Matti Hakama
Eero Pukkala
Author Affiliation
Finnish Cancer Registry, Institute for Statistical and Epidemiological Cancer Research, Liisankatu 21 B, FI-001 70 Helsinki, Finland. nea.malila@cancer.fi
Source
Acta Oncol. 2007;46(8):1103-6
Date
2007
Language
English
Publication Type
Article
Keywords
Cohort Studies
Colorectal Neoplasms - diagnosis - epidemiology - mortality
Feasibility Studies
Female
Finland
Follow-Up Studies
Humans
Incidence
Male
Mass Screening - methods
Occult Blood
Patient compliance
Reagent kits, diagnostic
Sensitivity and specificity
Abstract
The aim of the study was to assess the feasibility of and possible selection to attend in colorectal cancer screening.
During the years 1979-1980, 1 785 men and women (born in 1917-1929) were invited to a pilot screening project for colorectal cancer. The screening method used was a guaiac-based faecal occult blood test repeated once if the initial test was positive.
Compliance was 69% and the test was positive in 19% of those attending. In a record linkage with the Finnish Cancer Registry, 47 colorectal cancer cases and 24 deaths from colorectal cancer were observed by the end of 2004. In all, the particular test method was not regarded specific enough for population screening. There was, however, no difference in cancer incidence between those who complied and those who did not when compared to the general population of same age and gender.
Compliance was found high enough to make screening feasible and there was no self selection of persons with low cancer risk to attend screening.
PubMed ID
17851857 View in PubMed
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3423 records – page 1 of 343.