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DISTRIBUTION OF RNA-CONTAINING BEE VIRUSES IN HONEY BEE (APIS MELLIFERA) IN SEVERAL REGIONS OF RUSSIA.

https://arctichealth.org/en/permalink/ahliterature297772
Source
Mol Gen Mikrobiol Virusol. 2017; 35(1):31-35
Publication Type
Journal Article
Author
A E Kalashnikov
I G Udina
Source
Mol Gen Mikrobiol Virusol. 2017; 35(1):31-35
Language
English
Russian
Publication Type
Journal Article
Keywords
Animals
Bees - virology
RNA Viruses - genetics
Reverse Transcriptase Polymerase Chain Reaction
Russia
Virus Diseases - genetics - veterinary
Abstract
In several regions of Russia, broad distribution of RNA-containing bee viruses was found at apiaries of honey bee Apis mellifera using RT-PCR. Detected RNA-containing bee viruses are transferred simultaneously with invasion of mite Varroa destructor and lead to mass bee mortality that results in economic losses in bee breeding. In samples of Varroa destructor, bee viruses DWV and ABPV were found. High degree of RNA-containing virus (BQCV, DWV SBV, ABPV, CBPV and KBV) infection was revealed: in the average, at least 50% for bee families with mite infection. In the bee families studied in this work, mixed infection with 2-6 viruses simultaneously was detected. Amplified fragments of viruses BQCV, DWV and SBV obtained using RT-PCR were sequenced and registered in Genbank.
PubMed ID
30561942 View in PubMed
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Heterobasidion Partitivirus 13 Mediates Severe Growth Debilitation and Major Alterations in the Gene Expression of a Fungal Forest Pathogen.

https://arctichealth.org/en/permalink/ahliterature291284
Source
J Virol. 2018 03 01; 92(5):
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Date
03-01-2018
Author
Eeva J Vainio
Jaana Jurvansuu
Rafiqul Hyder
Muhammad Kashif
Tuula Piri
Tero Tuomivirta
Anna Poimala
Ping Xu
Salla Mäkelä
Dina Nitisa
Jarkko Hantula
Author Affiliation
Natural Resources Institute Finland, Helsinki, Finland eeva.vainio@luke.fi.
Source
J Virol. 2018 03 01; 92(5):
Date
03-01-2018
Language
English
Publication Type
Journal Article
Research Support, Non-U.S. Gov't
Keywords
Atropine - metabolism
Basidiomycota - genetics - growth & development - pathogenicity - virology
Biological Control Agents
Carbohydrate Metabolism
Cell Cycle
Diazepam - metabolism
Drug Combinations
Emodin - analogs & derivatives - metabolism
Europe
Forests
Gene Expression Regulation, Fungal
Genotype
Host-Pathogen Interactions - genetics - physiology
Metabolism
Mitochondria - metabolism
Mycelium - genetics - growth & development - virology
Norway
Phenotype
Phenylpropanolamine - metabolism
Picea - microbiology
Plant Diseases - economics - microbiology
RNA Virus Infections
RNA Viruses - genetics - physiology
RNA, Viral - genetics - isolation & purification
Sequence Analysis, RNA
Triiodothyronine - metabolism
Abstract
The fungal genus Heterobasidion includes some of the most devastating conifer pathogens in the boreal forest region. In this study, we showed that the alphapartitivirus Heterobasidion partitivirus 13 from Heterobasidion annosum (HetPV13-an1) is the main causal agent of severe phenotypic debilitation in the host fungus. Based on RNA sequencing using isogenic virus-infected and cured fungal strains, HetPV13-an1 affected the transcription of 683 genes, of which 60% were downregulated and 40% upregulated. Alterations observed in carbohydrate and amino acid metabolism suggest that the virus causes a state of starvation, which is compensated for by alternative synthesis routes. We used dual cultures to transmit HetPV13-an1 into new strains of H. annosum and Heterobasidion parviporum The three strains of H. parviporum that acquired the virus showed noticeable growth reduction on rich culturing medium, while only two of six H. annosum isolates tested showed significant debilitation. Based on reverse transcription-quantitative PCR (RT-qPCR) analysis, the response toward HetPV13-an1 infection was somewhat different in H. annosum and H. parviporum We assessed the effects of HetPV13-an1 on the wood colonization efficacy of H. parviporum in a field experiment where 46 Norway spruce trees were inoculated with isogenic strains with or without the virus. The virus-infected H. parviporum strain showed considerably less growth within living trees than the isolate without HetPV13-an1, indicating that the virus also causes growth debilitation in natural substrates.IMPORTANCE A biocontrol method restricting the spread of Heterobasidion species would be highly beneficial to forestry, as these fungi are difficult to eradicate from diseased forest stands and cause approximate annual losses of €800 million in Europe. We used virus curing and reintroduction experiments and RNA sequencing to show that the alphapartitivirus HetPV13-an1 affects many basic cellular functions of the white rot wood decay fungus Heterobasidion annosum, which results in aberrant hyphal morphology and a low growth rate. Dual fungal cultures were used to introduce HetPV13-an1 into a new host species, Heterobasidion parviporum, and field experiments confirmed the capability of the virus to reduce the growth of H. parviporum in living spruce wood. Taken together, our results suggest that HetPV13-an1 shows potential for the development of a future biocontrol agent against Heterobasidion fungi.
Notes
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PubMed ID
29237832 View in PubMed
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Pathogenicity of nodavirus strains from striped jack Pseudocaranx dentex and Atlantic halibut Hippoglossus hippoglossus, studied by waterborne challenge of yolk-sac larvae of both teleost species.

https://arctichealth.org/en/permalink/ahliterature50896
Source
Dis Aquat Organ. 1999 Nov 30;38(3):169-75
Publication Type
Article
Date
Nov-30-1999
Author
G K Totland
S. Grotmol
Y. Morita
T. Nishioka
T. Nakai
Author Affiliation
Department of Zoology, University of Bergen, Norway. geir.totland@zoo.uib.no
Source
Dis Aquat Organ. 1999 Nov 30;38(3):169-75
Date
Nov-30-1999
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Viral - chemistry
Antigens, Viral - chemistry - isolation & purification
Brain - virology
Comparative Study
Eye - virology
Fish Diseases - virology
Flatfishes
Fluorescent Antibody Technique, Indirect - veterinary
Immunohistochemistry
Japan
Microscopy, Electron
Norway
RNA Virus Infections - veterinary - virology
RNA Viruses - genetics - isolation & purification - pathogenicity
Research Support, Non-U.S. Gov't
Reverse Transcriptase Polymerase Chain Reaction - veterinary
Variation (Genetics) - genetics
Virulence
Yolk Sac - virology
Abstract
The present study shows that differences in pathogenicity exist among fish nodavirus strains. In challenge trials, a Japanese strain (SJ93Nag) was highly virulent to larvae of the striped jack Pseudocaranx dentex but replication was not detected in larvae of Atlantic halibut Hippoglossus hippoglossus at 6 degrees C. Conversely, a Norwegian nodavirus strain (AH95NorA) that was highly virulent to the Atlantic halibut larvae did not replicate in striped jack larvae at 20 degrees C. Occurrence of the disease viral encephalopathy and retinopathy (VER) and cumulative mortality were significantly different in the 2 species when challenged with the 2 nodavirus strains. The presence of nodavirus in nervous tissue was monitored by immunohistochemical methods. Our results support the view that the genetic diversity among nodavirus strains reflects the existence of different viral phenotypes which may be adapted to infect different host species and/or for replicating at different temperatures. Fish nodaviruses represent surveyable pathogens well suited for studying the relation between viral genotypic and phenotypic properties such as host specificity, temperature optima, neuroinvasiveness and neurovirulence.
PubMed ID
10686668 View in PubMed
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