Phospholipids and cholesterol were found to be the main lipids in mature and immature neuroblastoma cells. The ratios for the total cholesterol/phospholipids in these undifferentiated and differentiated cells were 0.33 and 0.52, respectively. The ratios of 0.45 and 0.62 were obtained with corresponding plasma membrane fractions. Individual fatty acid contents in the loosely bound lipid fraction were higher than in tightly bound lipids. The total levels of saturated fatty acids increased in both of these fractions. While arachidonic acid content significantly decreased, it increased simultaneously (600%) in the free fatty acid fraction during differentiation. The amount of cholesterol esters increased three-fold as a result of maturation. For the first time it was possible to detect, in neuroblastoma cells, several lipids, namely N-acylphosphatidylethanolamine, N-acylethanolamine and semilysobisphosphatidic acid. They all changed during maturation. Total N-acylphosphatidylethanolamine content decreased by 50%, disappearing completely from membrane fractions. N-Acylethanolamine disappeared from the cell as well as from membrane fractions. On the other hand the total cellular content of semilysobisphosphatidic acid increased without any alterations in its membrane content. Functional implications of our investigations are discussed.
Ischemic heart desease (IHD) remains actual issue of the current clinical medicine, because, IHD takes a leading position among reasons of death rate of population. Purpose of the study was to find diagnostic features of fat-acid composition of lipid of thrombocytes in patients with stable and unstable course of ischemic heart disease (IHD). 52 patients with an unstable angina pectoris aged 42-65 years and 31 patients with stable angina aged 41-66 years have been observed. Reliable changes in relation of the sum of saturated, unsaturated and poli-unsaturated of fat acids of phosphotides of thrombocytes in patients with unstable course of ischemic heart disease (IHD) have been detected. It improves knowledge about the mechanisms of destabilization of coronal circulation of blood.
BACKGROUND. To further characterize selected pathologic features on a biochemical level, the authors analyzed the nuclear magnetic resonance metabolite and phospholipid spectra of 30 malignant colon tumors using 31P magnetic resonance spectroscopy. METHODS. Eleven individual generic phospholipids were identified in the spectra of 17 phospholipid extracts, and 31 individual phosphatic metabolites were identified in the spectra of 13 perchloric acid extracts. The metabolites and lipids were quantified for statistical intergroup comparisons based on tumor stage, lymph node status, differentiation, mucin production, blood vessel invasion (BVI), and lymphatic vessel invasion (LVI). RESULTS. Significant elevations in the relative concentration of alpha-glycerol phosphate were noted when comparing AJCC tumor classification (T3 vs. T2, 0.92 +/- 0.14 vs. 0.46 +/- 0.11, P
The purpose of this study was to examine changes in binding properties of alpha 1-adrenoceptors, beta-adrenoceptors and L-Ca channels in rat heart muscle in relation to changes in the lipid environment within the membrane, i.e. fatty acyl chain composition of membrane phospholipids, occurring during repeated adrenergic stimulation. The effect of daily administration of epinephrine for seven days upon the maximum number of binding sites of adrenoceptors and upon the fatty acyl chain composition of phosphatidylcholine and phosphatidylethanolamine was examined in 10 months old rats. Decrease in Bmax of adrenoceptors during repeated adrenergic stimulation coincided with remodeling of the membrane phospholipids, with replacement of 18:2n-6 by 20:4n-6 in phosphatidylcholine and by 22:6n-3 in phosphatidylethanolamine, respectively. The effect of repeated adrenergic stimulation was also examined in rats fed different dietary fats and oils, i.e. butter, corn oil or cod liver oil, in hearts with markedly different levels of 18:2n-6, 20:4n-6 and 22:6n-3. The binding properties of the adrenoceptors and L-Ca channels did not relate to the fatty acyl chain composition of bulk phospholipids, but the epinephrine induced decrease in Bmax of the receptors was always accompanied by replacement of 18:2n-6 by 20:4n-6 in phosphatidylcholine and by 22:6n-3 in phosphatidylethanolamine, regardless of the initial level of these fatty acyl chains in the phospholipids. It is concluded that adaption to repeated adrenergic stimulation may include coregulation of the lipid environment within the membrane and the binding properties of adrenoceptors, and possibly other functionally coupled proteins such as L-Ca channels, residing in the membrane.
BACKGROUND: Choline and betaine are linked to phospholipid and one-carbon metabolism. Blood concentrations or dietary intake of these quaternary amines have been related to the risk of chronic diseases, including cardiovascular disease and the metabolic syndrome. OBJECTIVE: We aimed to determine dietary predictors of plasma choline and betaine among middle-aged and elderly subjects recruited from an area without folic acid fortification. DESIGN: This is a population-based study of 5812 men and women aged 47-49 and 71-74 y, within the Hordaland Health Study cohort. Plasma concentrations per increasing quartile of intake of foods, beverages, and nutrients were assessed by multiple linear regression analysis, and dietary patterns were assessed by factor analysis. RESULTS: Plasma choline was predicted by egg consumption (0.16 micromol/L; P
Lipid composition of plasma membranes of placental epithelial cells of villous chorion of healthy women and those with chlamydiosis has been studied. Lipid composition of plasma membranes of ill women differs from that of healthy women by reduction of total phospholipids quantity, by the increase of the level of free cholesterol and free fatty acids. A disturbance in the ratio between certain lipid fractions and increasing quantity of lysophospholipids is observed. Two-fold oppression of plasma membranes Na+, K+ -ATPase activity of placental epithelial cells of villous chorion in ill women has been detected but Mg2+, Ca2+ -ATPase activity has not changed. Thus chlamydial infection causes significant disorders in lipid composition and functioning of epithelial cells membranes of chorion.
The influence of irradiation and NSE on the microsomal lipid composition of the rat liver and heart was studied. It was shown, that radiation treatment in a dose of 2 Gy had not a significant affect on the heart phospholipid composition, whereas in the liver the amounts of the phosphatidylethnolamine and phosphatidylinositol were increased and the amounts of the phosphatidylcholine and sphyngomieline were decreased. NSE did not impact on these characteristics. The alterations of the plasmalogen/diacyl forms ratio of the PC and PE also took place only in the liver microsome. The analysis of the fatty acids esterified to phospholipids showed similar situation: the fatty acids of the heart microsome less were changed than those of the liver microsome undergo irradiation. The amount of arachidonic acid rise after the adding of NSE to rats. Investigation of the important component of biological membranes--cholesterol and its esters also showed that the liver tissue is more vulnerable to irradiation than the heart. NSE led to insignificant increase of the not esterified cholesterol in the liver. As a result of the present work we may conclude that the liver microsome is more vulnerable than the heart under X-ray radiation and NSE has protective effect in these conditions.
The purpose of this study was to examine the influence of ageing on the alterations in binding characteristics of adrenoceptors and membrane phospholipid fatty acids in rat heart following repeated administration of epinephrine. The maximal number of binding sites (Bmax) and dissociation constant (Kd) of [3H]prazosin and [3H]dihydroalprenolol binding to alpha 1- and beta-adrenoceptors, respectively, changed significantly during ageing. The downregulation of alpha 1- and beta-adrenoceptors after repeated epinephrine administration for one week, did not differ with age, but the response of the affinity (1/Kd) of both alpha 1- and beta-adrenoceptors to epinephrine treatment was age dependent. In 3-month-old rats the affinity of alpha 1-adrenoceptors was decreased after epinephrine treatment but the affinity of beta-adrenoceptors was unchanged. In 10- and 23-month-old rats the affinity of beta-adrenoceptors decreased after epinephrine treatment but the affinity of alpha 1-adrenoceptors did not change. During ageing the linoleic acid (18:2(n-6)) level decreased in phosphatidylcholine and the arachidonic acid (20:4(n-6)) level increased in phosphatidylcholine and phosphatidylethanolamine. After epinephrine administration the 18:2(n-6) level decreased and the docosahexaenoic acid (22:6(n-3)) level increased in phosphatidylcholine and phosphatidylethanolamine and those changes were not age dependent. The 20:4(n-6) level increased in phosphatidylcholine after epinephrine administration, but that increase was smaller in old than in young rats. The results show that both ageing and epinephrine administration simultaneously modify the fatty acid composition of membrane phospholipids and the binding properties of alpha 1- and beta-adrenoceptors in rat heart.