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31 records – page 1 of 4.

Changes in lipid composition of neuroblastoma C1300 N18 cell during differentiation.

https://arctichealth.org/en/permalink/ahliterature25714
Source
Neuroscience. 1989;30(1):153-64
Publication Type
Article
Date
1989
Author
N M Gulaya
G L Volkov
V M Klimashevsky
N N Govseeva
A A Melnik
Author Affiliation
A.V. Palladine Institute of Biochemistry, Academy of Sciences, Ukrainian SSR, Kiev.
Source
Neuroscience. 1989;30(1):153-64
Date
1989
Language
English
Publication Type
Article
Keywords
Animals
Arachidonic Acid
Arachidonic Acids - metabolism
Cell Differentiation
Cell Line
Fatty Acids - metabolism
Membrane Lipids - metabolism
Mice
Neuroblastoma
Phospholipids - metabolism
Tumor Cells, Cultured - cytology - metabolism
Abstract
Phospholipids and cholesterol were found to be the main lipids in mature and immature neuroblastoma cells. The ratios for the total cholesterol/phospholipids in these undifferentiated and differentiated cells were 0.33 and 0.52, respectively. The ratios of 0.45 and 0.62 were obtained with corresponding plasma membrane fractions. Individual fatty acid contents in the loosely bound lipid fraction were higher than in tightly bound lipids. The total levels of saturated fatty acids increased in both of these fractions. While arachidonic acid content significantly decreased, it increased simultaneously (600%) in the free fatty acid fraction during differentiation. The amount of cholesterol esters increased three-fold as a result of maturation. For the first time it was possible to detect, in neuroblastoma cells, several lipids, namely N-acylphosphatidylethanolamine, N-acylethanolamine and semilysobisphosphatidic acid. They all changed during maturation. Total N-acylphosphatidylethanolamine content decreased by 50%, disappearing completely from membrane fractions. N-Acylethanolamine disappeared from the cell as well as from membrane fractions. On the other hand the total cellular content of semilysobisphosphatidic acid increased without any alterations in its membrane content. Functional implications of our investigations are discussed.
PubMed ID
2501710 View in PubMed
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[Changes of fatty acid spectrum of thrombocyte lipids in patients with a stable and unstable angina]

https://arctichealth.org/en/permalink/ahliterature97039
Source
Lik Sprava. 2009 Oct-Dec;(7-8):30-2
Publication Type
Article
Author
V H Lyzohub
T V Zaval's'ka
Kh A Kh Abu Sara
T S Briuzhina
O V Savchenko
Source
Lik Sprava. 2009 Oct-Dec;(7-8):30-2
Language
Ukrainian
Publication Type
Article
Keywords
Adult
Aged
Angina Pectoris - blood - metabolism
Angina, Unstable - blood - metabolism
Blood Platelets - metabolism
Case-Control Studies
Fatty Acids - metabolism
Humans
Middle Aged
Phospholipids - metabolism
Abstract
Ischemic heart desease (IHD) remains actual issue of the current clinical medicine, because, IHD takes a leading position among reasons of death rate of population. Purpose of the study was to find diagnostic features of fat-acid composition of lipid of thrombocytes in patients with stable and unstable course of ischemic heart disease (IHD). 52 patients with an unstable angina pectoris aged 42-65 years and 31 patients with stable angina aged 41-66 years have been observed. Reliable changes in relation of the sum of saturated, unsaturated and poli-unsaturated of fat acids of phosphotides of thrombocytes in patients with unstable course of ischemic heart disease (IHD) have been detected. It improves knowledge about the mechanisms of destabilization of coronal circulation of blood.
PubMed ID
20455444 View in PubMed
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Characterization of malignant colon tumors with 31P nuclear magnetic resonance phospholipid and phosphatic metabolite profiles.

https://arctichealth.org/en/permalink/ahliterature4070
Source
Cancer. 1995 Nov 15;76(10):1715-23
Publication Type
Article
Date
Nov-15-1995
Author
T E Merchant
P M Diamantis
G. Lauwers
T. Haida
J N Kasimos
J. Guillem
T. Glonek
B D Minsky
Author Affiliation
Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Source
Cancer. 1995 Nov 15;76(10):1715-23
Date
Nov-15-1995
Language
English
Publication Type
Article
Keywords
Cell Differentiation
Colonic Neoplasms - metabolism - pathology
Humans
Lymphatic Metastasis
Magnetic Resonance Spectroscopy
Neoplasm Invasiveness
Phosphates - metabolism
Phospholipids - metabolism
Prognosis
Research Support, Non-U.S. Gov't
Abstract
BACKGROUND. To further characterize selected pathologic features on a biochemical level, the authors analyzed the nuclear magnetic resonance metabolite and phospholipid spectra of 30 malignant colon tumors using 31P magnetic resonance spectroscopy. METHODS. Eleven individual generic phospholipids were identified in the spectra of 17 phospholipid extracts, and 31 individual phosphatic metabolites were identified in the spectra of 13 perchloric acid extracts. The metabolites and lipids were quantified for statistical intergroup comparisons based on tumor stage, lymph node status, differentiation, mucin production, blood vessel invasion (BVI), and lymphatic vessel invasion (LVI). RESULTS. Significant elevations in the relative concentration of alpha-glycerol phosphate were noted when comparing AJCC tumor classification (T3 vs. T2, 0.92 +/- 0.14 vs. 0.46 +/- 0.11, P
PubMed ID
8625039 View in PubMed
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Coregulation of adrenoceptors and the lipid environment in heart muscle during repeated adrenergic stimulation.

https://arctichealth.org/en/permalink/ahliterature11475
Source
J Mol Cell Cardiol. 1995 Jan;27(1):243-51
Publication Type
Article
Date
Jan-1995
Author
S. Gudbjarnason
V E Benediktsdóttir
Author Affiliation
University of Iceland, Science Institute, Reykjavik.
Source
J Mol Cell Cardiol. 1995 Jan;27(1):243-51
Date
Jan-1995
Language
English
Publication Type
Article
Keywords
Animals
Butter
Cod Liver Oil
Comparative Study
Corn Oil
Dietary Fats - pharmacology
Epinephrine - pharmacology
Heart - drug effects - physiology
Kinetics
Male
Membrane Lipids - metabolism
Myocardium - metabolism
Phosphatidylcholines - metabolism
Phosphatidylethanolamines - metabolism
Phospholipids - metabolism
Rats
Rats, Wistar
Receptors, Adrenergic, alpha-1 - metabolism
Receptors, Adrenergic, beta - metabolism
Sarcolemma - drug effects - metabolism - physiology
Sodium Channels - metabolism
Abstract
The purpose of this study was to examine changes in binding properties of alpha 1-adrenoceptors, beta-adrenoceptors and L-Ca channels in rat heart muscle in relation to changes in the lipid environment within the membrane, i.e. fatty acyl chain composition of membrane phospholipids, occurring during repeated adrenergic stimulation. The effect of daily administration of epinephrine for seven days upon the maximum number of binding sites of adrenoceptors and upon the fatty acyl chain composition of phosphatidylcholine and phosphatidylethanolamine was examined in 10 months old rats. Decrease in Bmax of adrenoceptors during repeated adrenergic stimulation coincided with remodeling of the membrane phospholipids, with replacement of 18:2n-6 by 20:4n-6 in phosphatidylcholine and by 22:6n-3 in phosphatidylethanolamine, respectively. The effect of repeated adrenergic stimulation was also examined in rats fed different dietary fats and oils, i.e. butter, corn oil or cod liver oil, in hearts with markedly different levels of 18:2n-6, 20:4n-6 and 22:6n-3. The binding properties of the adrenoceptors and L-Ca channels did not relate to the fatty acyl chain composition of bulk phospholipids, but the epinephrine induced decrease in Bmax of the receptors was always accompanied by replacement of 18:2n-6 by 20:4n-6 in phosphatidylcholine and by 22:6n-3 in phosphatidylethanolamine, regardless of the initial level of these fatty acyl chains in the phospholipids. It is concluded that adaption to repeated adrenergic stimulation may include coregulation of the lipid environment within the membrane and the binding properties of adrenoceptors, and possibly other functionally coupled proteins such as L-Ca channels, residing in the membrane.
PubMed ID
7760348 View in PubMed
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Dietary patterns, food groups, and nutrients as predictors of plasma choline and betaine in middle-aged and elderly men and women.

https://arctichealth.org/en/permalink/ahliterature90912
Source
Am J Clin Nutr. 2008 Dec;88(6):1663-9
Publication Type
Article
Date
Dec-2008
Author
Konstantinova Svetlana V
Tell Grethe S
Vollset Stein E
Ulvik Arve
Drevon Christian A
Ueland Per M
Author Affiliation
Department of Public Health and Primary Health Care, University of Bergen, Bergen, Norway. svetlana.konstantinova@isf.uib.no
Source
Am J Clin Nutr. 2008 Dec;88(6):1663-9
Date
Dec-2008
Language
English
Publication Type
Article
Keywords
Aged
Betaine - administration & dosage - blood
Bread - analysis
Cardiovascular Diseases - blood - epidemiology - etiology
Choline - administration & dosage - blood
Cohort Studies
Diet Surveys
Dietary Fats - administration & dosage
Eggs - analysis
Factor Analysis, Statistical
Female
Folic Acid - administration & dosage
Food Habits
Food, Fortified
Humans
Linear Models
Male
Meat
Metabolic Syndrome X - blood - epidemiology - etiology
Middle Aged
Norway - epidemiology
Phospholipids - metabolism
Abstract
BACKGROUND: Choline and betaine are linked to phospholipid and one-carbon metabolism. Blood concentrations or dietary intake of these quaternary amines have been related to the risk of chronic diseases, including cardiovascular disease and the metabolic syndrome. OBJECTIVE: We aimed to determine dietary predictors of plasma choline and betaine among middle-aged and elderly subjects recruited from an area without folic acid fortification. DESIGN: This is a population-based study of 5812 men and women aged 47-49 and 71-74 y, within the Hordaland Health Study cohort. Plasma concentrations per increasing quartile of intake of foods, beverages, and nutrients were assessed by multiple linear regression analysis, and dietary patterns were assessed by factor analysis. RESULTS: Plasma choline was predicted by egg consumption (0.16 micromol/L; P
PubMed ID
19064529 View in PubMed
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[Disorders in lipid composition and properties of plasma membrane in epithelial cells of the placental villous chorion in chlamydial infection]

https://arctichealth.org/en/permalink/ahliterature91875
Source
Ukr Biokhim Zh. 2008 Mar-Apr;80(2):135-40
Publication Type
Article
Author
Iablons'ka S V
Fartushok T V
Vesel's'kyi S P
Kondratiuk O A
Besedin V M
Rybal'chenko V K
Source
Ukr Biokhim Zh. 2008 Mar-Apr;80(2):135-40
Language
Ukrainian
Publication Type
Article
Keywords
Ca(2+) Mg(2+)-ATPase - metabolism
Cell Membrane - enzymology - metabolism
Chlamydia Infections - metabolism
Chorionic Villi - metabolism
Epithelial Cells - enzymology - metabolism
Female
Humans
Membrane Lipids - metabolism
Phospholipids - metabolism
Pregnancy
Pregnancy Complications, Infectious - metabolism
Sodium-Potassium-Exchanging ATPase - metabolism
Abstract
Lipid composition of plasma membranes of placental epithelial cells of villous chorion of healthy women and those with chlamydiosis has been studied. Lipid composition of plasma membranes of ill women differs from that of healthy women by reduction of total phospholipids quantity, by the increase of the level of free cholesterol and free fatty acids. A disturbance in the ratio between certain lipid fractions and increasing quantity of lysophospholipids is observed. Two-fold oppression of plasma membranes Na+, K+ -ATPase activity of placental epithelial cells of villous chorion in ill women has been detected but Mg2+, Ca2+ -ATPase activity has not changed. Thus chlamydial infection causes significant disorders in lipid composition and functioning of epithelial cells membranes of chorion.
PubMed ID
18819385 View in PubMed
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[Effect of N-stearoylethanolamine and ionizing radiation on lipid components of the liver and heart microsomes in rats]

https://arctichealth.org/en/permalink/ahliterature9562
Source
Ukr Biokhim Zh. 2003 Jul-Aug;75(4):81-90
Publication Type
Article
Author
M V Artamonov
O D Zhukov
T M Horid'ko
V M Klimashevs'kyi
O P Martseniuk
N M Hula
Author Affiliation
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. artamon@biochem.kiev.ua
Source
Ukr Biokhim Zh. 2003 Jul-Aug;75(4):81-90
Language
Ukrainian
Publication Type
Article
Keywords
Administration, Oral
Animals
English Abstract
Ethanolamines - administration & dosage - pharmacology
Heart - drug effects - radiation effects
Microsomes - drug effects - metabolism - radiation effects
Microsomes, Liver - drug effects - metabolism - radiation effects
Organ Specificity
Phospholipids - metabolism
Radiation-Protective Agents - administration & dosage - pharmacology
Rats
Rats, Wistar
Stearic Acids - administration & dosage - pharmacology
Whole-Body Irradiation
X-rays
Abstract
The influence of irradiation and NSE on the microsomal lipid composition of the rat liver and heart was studied. It was shown, that radiation treatment in a dose of 2 Gy had not a significant affect on the heart phospholipid composition, whereas in the liver the amounts of the phosphatidylethnolamine and phosphatidylinositol were increased and the amounts of the phosphatidylcholine and sphyngomieline were decreased. NSE did not impact on these characteristics. The alterations of the plasmalogen/diacyl forms ratio of the PC and PE also took place only in the liver microsome. The analysis of the fatty acids esterified to phospholipids showed similar situation: the fatty acids of the heart microsome less were changed than those of the liver microsome undergo irradiation. The amount of arachidonic acid rise after the adding of NSE to rats. Investigation of the important component of biological membranes--cholesterol and its esters also showed that the liver tissue is more vulnerable to irradiation than the heart. NSE led to insignificant increase of the not esterified cholesterol in the liver. As a result of the present work we may conclude that the liver microsome is more vulnerable than the heart under X-ray radiation and NSE has protective effect in these conditions.
PubMed ID
14681979 View in PubMed
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Effects of ageing and adrenergic stimulation on alpha 1- and beta-adrenoceptors and phospholipid fatty acids in rat heart.

https://arctichealth.org/en/permalink/ahliterature11398
Source
Eur J Pharmacol. 1995 May 26;289(3):419-27
Publication Type
Article
Date
May-26-1995
Author
V E Benediktsdóttir
G V Skúladóttir
S. Gudbjarnason
Author Affiliation
Science Institute, University of Iceland, Reykjavík.
Source
Eur J Pharmacol. 1995 May 26;289(3):419-27
Date
May-26-1995
Language
English
Publication Type
Article
Keywords
Adrenergic beta-Agonists - pharmacology
Aging - metabolism
Animals
Catecholamines - metabolism
Dihydroalprenolol - diagnostic use
Epinephrine - pharmacology
Fatty Acids - metabolism
Heart - drug effects
In Vitro
Kinetics
Male
Membranes - drug effects - metabolism
Myocardium - metabolism
Phosphatidylethanolamines - metabolism
Phospholipids - metabolism
Rats
Rats, Wistar
Receptors, Adrenergic, alpha-1 - agonists
Abstract
The purpose of this study was to examine the influence of ageing on the alterations in binding characteristics of adrenoceptors and membrane phospholipid fatty acids in rat heart following repeated administration of epinephrine. The maximal number of binding sites (Bmax) and dissociation constant (Kd) of [3H]prazosin and [3H]dihydroalprenolol binding to alpha 1- and beta-adrenoceptors, respectively, changed significantly during ageing. The downregulation of alpha 1- and beta-adrenoceptors after repeated epinephrine administration for one week, did not differ with age, but the response of the affinity (1/Kd) of both alpha 1- and beta-adrenoceptors to epinephrine treatment was age dependent. In 3-month-old rats the affinity of alpha 1-adrenoceptors was decreased after epinephrine treatment but the affinity of beta-adrenoceptors was unchanged. In 10- and 23-month-old rats the affinity of beta-adrenoceptors decreased after epinephrine treatment but the affinity of alpha 1-adrenoceptors did not change. During ageing the linoleic acid (18:2(n-6)) level decreased in phosphatidylcholine and the arachidonic acid (20:4(n-6)) level increased in phosphatidylcholine and phosphatidylethanolamine. After epinephrine administration the 18:2(n-6) level decreased and the docosahexaenoic acid (22:6(n-3)) level increased in phosphatidylcholine and phosphatidylethanolamine and those changes were not age dependent. The 20:4(n-6) level increased in phosphatidylcholine after epinephrine administration, but that increase was smaller in old than in young rats. The results show that both ageing and epinephrine administration simultaneously modify the fatty acid composition of membrane phospholipids and the binding properties of alpha 1- and beta-adrenoceptors in rat heart.
PubMed ID
7556410 View in PubMed
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31 records – page 1 of 4.