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Adoptive transfer of allergic airway responses with sensitized lymphocytes in BN rats.

https://arctichealth.org/en/permalink/ahliterature57674
Source
Am J Respir Crit Care Med. 1995 Jul;152(1):64-70
Publication Type
Article
Date
Jul-1995
Author
A. Watanabe
P. Rossi
P M Renzi
L J Xu
R D Guttmann
J G Martin
Author Affiliation
Meakins-Christie Laboratories, McGill University, Royal Victoria Hospital, Montreal, Quebec, Canada.
Source
Am J Respir Crit Care Med. 1995 Jul;152(1):64-70
Date
Jul-1995
Language
English
Publication Type
Article
Keywords
Animals
Bronchial Hyperreactivity - immunology - physiopathology
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid - cytology
Eosinophils - immunology
Immunoglobulin E - immunology
Immunotherapy, Adoptive
Male
Ovalbumin - immunology
Passive Cutaneous Anaphylaxis - immunology
Rats
Rats, Inbred BN
Rats, Sprague-Dawley
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - immunology - physiopathology
Serum Albumin, Bovine - immunology
T-Lymphocytes - immunology
Abstract
To evaluate the role of lymphocytes in the pathogenesis of allergic bronchoconstriction, we investigated whether allergic airway responses are adoptively transferred by antigen-primed lymphocytes in Brown Norway (BN) rats. Animals were actively sensitized to ovalbumin (OA) or sham sensitized, and 14 d later mononuclear cells (MNCs) were isolated from intrathoracic lymph nodes, passed through a nylon wool column, and transferred to naive syngeneic rats. Recipients were challenged with aerosolized OA or bovine serum albumin (BSA) (5% wt/vol) and analyzed for changes in lung resistance (RL), airway responsiveness to inhaled methacholine (MCh), and bronchoalveolar lavage (BAL) cells. Recipients of MNCs from sensitized rats responded to OA inhalation and exhibited sustained increases in RL throughout the 8-h observation period, but without usual early airway responses. Recipients of sham-sensitized MNCs or BSA-challenged recipients failed to respond to antigen challenge. At 32 h after OA exposure, airway responsiveness to MCh was increased in four of seven rats that had received sensitized MNCs (p = 0.035). BAL eosinophils increased at 32 h in the recipients of both sensitized and sham-sensitized MNCs. However, eosinophil numbers in BAL were inversely correlated with airway responsiveness in the recipients of sensitized MNCs (r = -0.788, p = 0.036). OA-specific immunoglobulin E (IgE) was undetectable by enzyme-linked immunosorbent assay (ELISA) or passive cutaneous anaphylaxis (PCA) in recipient rats following adoptive transfer. In conclusion, allergic late airway responses (LAR) and cholinergic airway hyperresponsiveness, but not antigen-specific IgE and early responses, were adoptively transferred by antigen-primed lymphocytes in BN rats.(ABSTRACT TRUNCATED AT 250 WORDS)
PubMed ID
7599864 View in PubMed
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Brown Norway rat ovalbumin-specific immunoglobulin E antibodies increase the human basophil expression of CD63 marker.

https://arctichealth.org/en/permalink/ahliterature57432
Source
Scand J Immunol. 2003 Mar;57(3):271-8
Publication Type
Article
Date
Mar-2003
Author
A. Bellou
J. Saint-Laudy
L. Knippels
C. Montémont
E. Vauthier
P. Gerard
H. Pellegrom
E K Koerkamp
J F Lesesve
J L Guéant
H. Lambert
J P Mallié
Author Affiliation
Laboratoire de Néphrologie Expérimentale, UPRESS-JE2165, Faculté de Médecine de Nancy, Vandoeuvre les Nancy, France. abdel.bellou@voila.fr
Source
Scand J Immunol. 2003 Mar;57(3):271-8
Date
Mar-2003
Language
English
Publication Type
Article
Keywords
Anaphylaxis - immunology
Animals
Antigens, CD - biosynthesis - immunology
Basophils - cytology - immunology - metabolism
Enzyme-Linked Immunosorbent Assay
Histamine Release - immunology
Humans
Immunization
Immunoglobulin E - immunology
Ovalbumin - immunology
Passive Cutaneous Anaphylaxis - immunology
Platelet Membrane Glycoproteins - biosynthesis - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Abstract
Anaphylactic shock is an immunoglobulin E (IgE)-dependent hypersensitivity. Biological tests like leucocyte histamine release (LHR) and human basophil activation (HBA), frequently used in human allergy, reflect both the amount of IgE fixed on cells and the cellular reactivity. To assess whether serum-specific IgE from Brown Norway (BN) rats prepared for ovalbumin (OVA)-induced anaphylactic shocks can activate human basophils which has a potential interest in experimental allergy: such a test could rapidly assert an IgE sensitization in laboratory animals genetically T-helper 2 (Th2)-predisposed. Rats (n = 39) were immunized three times (day 0, day 5 and day 21) with OVA injected subcutaneously. One week after the third immunization, a shock was induced with an intravenous (i.v.) bolus of OVA. Sensitization was assessed by passive cutaneous anaphylaxis (PCA) test and dosages of serum IgE antibodies anti-OVA by enzyme-linked immunosorbent assay. Blood basophils were counted before and during the shock. Before the shock induction (at day 21), an LHR test was performed on rat blood, and human basophils were sensitized with rat sera. HBA was demonstrated by the increase in the percentage of cells expressing CD63 antigen membrane, measured by flow cytometry. Twenty-one days after the first subcutaneous (s.c.) immunization, the rat serum induced a significant HBA. HBA was observed neither with the same serum previously heated nor with the serum from nonimmunized rats (NIRs). OVA-specific IgEs were significantly increased in immunized rat (IR) serum. The PCA test was negative when the serum was previously heated (56 degrees C). We never observed any circulating basophils, and LHR test was negative. After OVA i.v. administration, all IRs died rapidly. HBA testing strongly suggests a mediation by specific IgE in the increase of CD63 in BN rats. Thus, HBA test seems useful in assessing whether an experimental allergy was induced in animals genetically predisposed to an immune response, Th2-mediated, like BN rat. We also conclude that rat basophil activation does not participate in the histamine release during anaphylactic shock in sensitized BN rats.
PubMed ID
12641656 View in PubMed
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Experimental immune-mediated blepharoconjunctivitis in rats induced by immunization with ragweed pollen.

https://arctichealth.org/en/permalink/ahliterature50871
Source
Graefes Arch Clin Exp Ophthalmol. 2000 Apr;238(4):346-51
Publication Type
Article
Date
Apr-2000
Author
H. Iwamoto
K. Nishino
T M Magone
S M Whitcup
O. Yoshida
H. Yoshida
A. Ozaki
A. Fukushima
H. Ueno
Author Affiliation
Department of Ophthalmology, Kochi Medical School, Nankoku, Japan.
Source
Graefes Arch Clin Exp Ophthalmol. 2000 Apr;238(4):346-51
Date
Apr-2000
Language
English
Publication Type
Article
Keywords
Allergens - adverse effects - immunology
Aluminum Hydroxide - adverse effects
Animals
Blepharitis - chemically induced - immunology - pathology
Comparative Study
Conjunctivitis - chemically induced - immunology - pathology
Emulsions
Enzyme-Linked Immunosorbent Assay
Eosinophils - immunology
Freund's Adjuvant - adverse effects
Immunization - methods
Immunoglobulin E - analysis
Immunoglobulin G - analysis
Interferon Type II - biosynthesis
Lymphocyte Activation - immunology
Male
Ovalbumin - immunology
Passive Cutaneous Anaphylaxis - immunology
Plant Proteins - adverse effects - immunology
Pollen - adverse effects - immunology
Rats
Rats, Inbred BN
Rats, Inbred Lew
Rats, Sprague-Dawley
Research Support, Non-U.S. Gov't
Th1 Cells - immunology
Th2 Cells - immunology
Abstract
BACKGROUND: A study was performed to compare the effects of immunization with ragweed pollen (RW) in two different adjuvants on the characteristics of a previously described model of experimental immune-mediated blepharoconjunctivitis (EC) in rats. METHODS: Lewis or Brown Norway (BN) rats were immunized with 100 microg of RW in emulsion with aluminum hydroxide [Al(OH)3] or complete Freund's adjuvant (CFA). Three weeks later, the animals were challenged with eye drops containing RW in PBS. Twenty-four hours after topical challenge, eyes, blood, and lymph nodes were obtained for histology, measurement of antigen-specific antibodies, and proliferation or cytokine assays, respectively. In addition to active immunization, recipients of RW-primed lymph node cells were challenged and evaluated as above. RESULTS: RW in both adjuvants induced infiltration with predominantly mononuclear cells in Lewis rats and eosinophils in BN rats. As well as active immunization, eosinophils were detected only in BN rats by adoptive transfer of cells. Lymphocyte proliferative responses to RW were high in immunized Lewis rats when CFA was used as an adjuvant. In contrast, proliferative responses in BN rats were higher when Al(OH)3 was used. RW-specific IgE was detected only in BN rats. There were no significant differences in RW-specific IgG1/IgG2a ratio among the four groups. Lewis rats had higher level of RW-specific interferon-gamma in the culture supernatant. CONCLUSIONS: The characteristics of EC are different in Lewis and BN rats, dependent on the genetic background of the rat strains. The response to RW was similar to other previously used antigens, such as ovalbumin.
PubMed ID
10853935 View in PubMed
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