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Cryptosporidium and Giardia in marine-foraging river otters (Lontra canadensis) from the Puget Sound Georgia Basin ecosystem.

https://arctichealth.org/en/permalink/ahliterature164131
Source
J Parasitol. 2007 Feb;93(1):198-202
Publication Type
Article
Date
Feb-2007
Author
J K Gaydos
W A Miller
K V K Gilardi
A. Melli
H. Schwantje
C. Engelstoft
H. Fritz
P A Conrad
Author Affiliation
Orcas Island Office, University of California-Davis Wildlife Health Center, 1016 Deer Harbor Road, Eastsound, Washington 98245, USA. jkgaydos@ucdavis.edu
Source
J Parasitol. 2007 Feb;93(1):198-202
Date
Feb-2007
Language
English
Publication Type
Article
Keywords
Animals
British Columbia - epidemiology
Cryptosporidiosis - epidemiology - transmission - veterinary
Cryptosporidium - classification - genetics - isolation & purification
Ecosystem
Feces - parasitology
Genotype
Giardia - classification - genetics - isolation & purification
Giardiasis - epidemiology - transmission - veterinary
Humans
Otters - parasitology
Risk factors
Washington - epidemiology
Zoonoses
Abstract
Species of Cryptosporidium and Giardia can infect humans and wildlife and have the potential to be transmitted between these 2 groups; yet, very little is known about these protozoans in marine wildlife. Feces of river otters (Lontra canadensis), a common marine wildlife species in the Puget Sound Georgia Basin, were examined for species of Cryptosporidium and Giardia to determine their role in the epidemiology of these pathogens. Using ZnSO4 flotation and immunomagnetic separation, followed by direct immunofluorescent antibody detection (IMS/DFA), we identified Cryptosporidium sp. oocysts in 9 fecal samples from 6 locations and Giardia sp. cysts in 11 fecal samples from 7 locations. The putative risk factors of proximate human population and degree of anthropogenic shoreline modification were not associated with the detection of Cryptosporidium or Giardia spp. in river otter feces. Amplification of DNA from the IMS/DFA slide scrapings was successful for 1 sample containing > 500 Cryptosporidium sp. oocysts. Sequences from the Cryptosporidium 18S rRNA and the COWP loci were most similar to the ferret Cryptosporidium sp. genotype. River otters could serve as reservoirs for Cryptosporidium and Giardia species in marine ecosystems. More work is needed to better understand the zoonotic potential of the genotypes they carry as well as their implications for river otter health.
PubMed ID
17436965 View in PubMed
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