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101 records – page 1 of 11.

The 68K protease has beta-secretase-like activity for lymphocyte precursor protein but not for brain substrate.

https://arctichealth.org/en/permalink/ahliterature199515
Source
Neuroreport. 2000 Feb 7;11(2):373-7
Publication Type
Article
Date
Feb-7-2000
Author
A. Matsumoto
Author Affiliation
Department of Radiation Biophysics and Genetics, Kobe University School of Medicine, Japan.
Source
Neuroreport. 2000 Feb 7;11(2):373-7
Date
Feb-7-2000
Language
English
Publication Type
Article
Keywords
Aged
Aged, 80 and over
Alzheimer Disease - enzymology
Amino Acid Sequence
Amyloid Precursor Protein Secretases
Amyloid beta-Protein Precursor - metabolism
Aspartic Acid Endopeptidases - metabolism
Blotting, Western
Cells, Cultured
Cerebral Cortex - enzymology
Chondroitin ABC Lyase - metabolism
Endopeptidases
Female
Humans
Isoenzymes - metabolism
Lymphocytes - cytology - enzymology
Male
Middle Aged
Molecular Sequence Data
Organ Specificity
Peptide Fragments - chemistry
Polysaccharide-Lyases - metabolism
Protein Processing, Post-Translational
Sequence Analysis, Protein
Serine Endopeptidases - metabolism
Substrate Specificity
Abstract
Processing and metabolism of beta-amyloid precursor protein (APP) and generation of a variety of beta-amyloid (Abeta) peptides in the human brain is essentially associated with pathophysiology of Alzheimer's disease (AD). APP degradation activity of the 68 kDa serine protease, which was originally prepared from familial AD lymphoblastoid cells and harbors beta-secretase-like activity, was analyzed by Western blot using anti Abeta 1/40 antibody and anti APP cytoplasmic domain (CT) antibody. Native lymphocyte APP (LAPP) prepared from normal or AD-derived lymphoblastoid cells was degraded by the protease, generating a 16 kDa Abeta-bearing C-terminal fragment of APP. N-terminal amino acid sequencing of the fragment indicated that the protease cleaves LAPP at the Abeta-N-terminus. When the LAPP was treated with chondroitinase ABC prior to proteolysis, the activity to generate the fragment was inhibited, but pretreatment with heparitinase resulted in no effect. Native hippocampal APP prepared from normal brain, however, did not generate the 16 kDa peptide by the protease treatment. These results suggest that the process of APP degradation and Abeta-peptides generation, including beta-secretase activity, is associated with tissue specificity of both APP substrate and proteases. They also indicate that sulfated glycoconjugates attached to a portion of APP isoforms may play a role as a molecular determinant in the proteolysis.
PubMed ID
10674489 View in PubMed
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Accumulation, organ distribution, and excretion kinetics of ²4¹Am in Mayak Production Association workers.

https://arctichealth.org/en/permalink/ahliterature116778
Source
Health Phys. 2013 Mar;104(3):313-24
Publication Type
Article
Date
Mar-2013
Author
Klara G Suslova
Alexandra B Sokolova
Alexander V Efimov
Scott C Miller
Author Affiliation
Southern Urals Biophysics Institute, Ozyorsk, Chelyabinsk Region, Russia. suslova@subi.su
Source
Health Phys. 2013 Mar;104(3):313-24
Date
Mar-2013
Language
English
Publication Type
Article
Keywords
Adult
Aerosols
Aged
Americium - chemistry - pharmacokinetics
Case-Control Studies
Female
Humans
Industry
Kinetics
Liver Diseases - metabolism
Male
Middle Aged
Nuclear Reactors - statistics & numerical data
Occupational Exposure - analysis
Organ Specificity
Plutonium - chemistry - pharmacokinetics
Russia
Tissue Distribution
Abstract
Americium-241 (²4¹Am) is the second most significant radiation hazard after ²³?Pu at some of the Mayak Production Association facilities. This study summarizes current data on the accumulation, distribution, and excretion of americium compared with plutonium in different organs from former Mayak PA workers. Americium and plutonium were measured in autopsy and bioassay samples and correlated with the presence or absence of chronic disease and with biological transportability of the aerosols encountered at different workplaces. The relative accumulation of ²4¹Am was found to be increasing in the workers over time. This is likely from ²4¹Pu that increases with time in reprocessed fuel and from the increased concentrations of ²4¹Am and ²4¹Pu in inhaled alpha-active aerosols. While differences were observed in lung retention with exposures to different industrial compounds with different transportabilities (i.e., dioxide and nitrates), there were no significant differences in lung retention between americium and plutonium within each transportability group. In the non-pulmonary organs, the highest ratios of ²4¹Am/²4¹Am + SPu were observed in the skeleton. The relative ratios of americium in the skeleton versus liver were significantly greater than for plutonium. The relative amounts of americium and plutonium found in the skeleton compared with the liver were even greater in workers with documented chronic liver diseases. Excretion rates of ²4¹Am in ‘‘healthy’’ workers were estimated using bioassay and autopsy data. The data suggest that impaired liver function leads to reduced hepatic ²4¹Am retention, leading to increased ²4¹Am excretion.
PubMed ID
23361427 View in PubMed
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[Activity of organ specific enzymes in animals with liver damage following administration of antihepatocytotoxic serum]

https://arctichealth.org/en/permalink/ahliterature57079
Source
Fiziol Zh. 1976 Sep-Oct;22(5):611-5
Publication Type
Article

Acute leukaemia and other secondary neoplasms in patients treated with conventional chemotherapy for multiple myeloma: a Finnish Leukaemia Group study.

https://arctichealth.org/en/permalink/ahliterature197408
Source
Eur J Haematol. 2000 Aug;65(2):123-7
Publication Type
Article
Date
Aug-2000
Source
Eur J Haematol. 2000 Aug;65(2):123-7
Date
Aug-2000
Language
English
Publication Type
Article
Keywords
Acute Disease
Aged
Antineoplastic Combined Chemotherapy Protocols - adverse effects - therapeutic use
Female
Finland - epidemiology
Follow-Up Studies
Humans
Leukemia - chemically induced - epidemiology
Lymphoma, Non-Hodgkin - chemically induced
Male
Melphalan - administration & dosage - adverse effects
Middle Aged
Multiple Myeloma - drug therapy - pathology
Neoplasm Staging
Neoplasms, Second Primary - chemically induced - epidemiology
Organ Specificity
Abstract
The occurrence of acute leukaemia and other secondary neoplasms in 432 patients treated with conventional chemotherapy for multiple myeloma was analysed after a follow-up period of 11-19 yr (mean 16 yr). The number and organ-specific distribution of observed solid neoplasms was close to that expected in the general population. Non-Hodgkin's lymphoma developed in three patients (expected 0.7, p = 0.19). Acute leukaemia was diagnosed in 14 patients with an actuarial risk of 9.8% at 9 yr. No further cases were diagnosed thereafter. The average numbers of courses (26.2 vs. 25.5) and cumulative doses of melphalan (1440 and 1400 mg) were similar in patients with or without acute leukaemia. It seems possible that the advanced stage of multiple myeloma is more vulnerable to the leukaemogenic effect of melphalan compared with the earlier stages.
PubMed ID
10966173 View in PubMed
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Adipose tissue distribution in relation to insulin sensitivity and inflammation in Pakistani and Norwegian subjects with type 2 diabetes.

https://arctichealth.org/en/permalink/ahliterature267601
Source
Scand J Clin Lab Invest. 2014 Nov;74(8):700-7
Publication Type
Article
Date
Nov-2014
Author
Cecilie Wium
Heidi B Eggesbø
Thor Ueland
Annika E Michelsen
Peter A Torjesen
Pål Aukrust
Kåre Birkeland
Source
Scand J Clin Lab Invest. 2014 Nov;74(8):700-7
Date
Nov-2014
Language
English
Publication Type
Article
Keywords
Abdominal Fat - pathology - radiography
Adipokines - blood
Adult
Body Fat Distribution
Diabetes Mellitus, Type 2 - blood - ethnology - pathology
Female
Humans
Inflammation Mediators - blood
Insulin Resistance
Male
Middle Aged
Muscle, Skeletal - pathology
Norway
Organ Specificity
Pakistan - ethnology
Abstract
Immigrants from South Asia to Western countries have a high prevalence of type 2 diabetes mellitus (T2DM) associated with obesity. We investigated the relationship between diabetes and adipose tissue distribution in a group of younger T2DM subjects from Norway and Pakistan. Eighteen immigrant Pakistani and 21 Norwegian T2DM subjects (age 29-45, 49% men) were included. They underwent anthropometrical measurements including bioelectrical impedance analysis, CT scans measuring fatty infiltration in liver and adipose and muscle tissue compartments in mid-abdomen and thigh, a euglycemic clamp, and blood samples for serum insulin and plasma glucose, adipokines and inflammation markers. Adipose tissue distribution was similar in Norwegians and Pakistanis. Pakistanis, but not Norwegians, showed a negative correlation between insulin sensitivity and visceral adipose tissue (VAT, rs = - 0.704, p = 0.003). Subcutaneous adipose tissue (SAT) correlated to leptin in both Pakistanis and Norwegians (rs = 0.88, p
Notes
Comment In: Scand J Clin Lab Invest. 2015 Sep;75(5):434-525874480
Comment In: Scand J Clin Lab Invest. 2015 Sep;75(5):438-925916836
Comment In: Scand J Clin Lab Invest. 2015 Sep;75(5):44025916835
PubMed ID
25223599 View in PubMed
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[Age-dependent cadmium accumulation in organs of intoxicated rats and changes of indices of acid-base balance of blood in different conditions of antioxidant defence]

https://arctichealth.org/en/permalink/ahliterature83019
Source
Ukr Biokhim Zh. 2004 Nov-Dec;76(6):95-9
Publication Type
Article
Author
Mel'nychuk D O
Mel'nykova N M
Derkach Ie A
Source
Ukr Biokhim Zh. 2004 Nov-Dec;76(6):95-9
Language
Ukrainian
Publication Type
Article
Keywords
Acid-Base Equilibrium - drug effects
Aging - blood - metabolism
Animals
Antioxidants - pharmacology
Cadmium Compounds - pharmacokinetics - toxicity
Organ Specificity
Rats
Rats, Wistar
Sulfates - pharmacokinetics - toxicity
Abstract
Age peculiarities of cadmium cumulation in the organs of rats under different conditions of antioxidants administration. Considerable hepato- and nefrotoxic effect of the given xenobiotic under different conditions of its supply into the organism was revealed. The strongly pronounced differences between the age distribution of cadmium in the organisms have been marked. It is proved that the most antioxidantal activity is shown by sodium selenite when administered into the organism before poisoning with cadmium. It is stated that the indices showing the acid-alkaline state are subject to the greatest changes in the bodies of young animals. In this case they have more expressed toxic effects under the influence of ions of heavy metals as well as the receptivity to the positive influence of antioxidants.
PubMed ID
16350750 View in PubMed
Less detail

Amyloid precursor protein mutation causes Alzheimer's disease in a Swedish family.

https://arctichealth.org/en/permalink/ahliterature218798
Source
Neurosci Lett. 1994 Feb 28;168(1-2):254-6
Publication Type
Article
Date
Feb-28-1994
Author
L. Lannfelt
N. Bogdanovic
H. Appelgren
K. Axelman
L. Lilius
G. Hansson
D. Schenk
J. Hardy
B. Winblad
Source
Neurosci Lett. 1994 Feb 28;168(1-2):254-6
Date
Feb-28-1994
Language
English
Publication Type
Article
Keywords
Aged
Alzheimer Disease - genetics - pathology
Amyloid beta-Protein Precursor - genetics
Brain - pathology
Female
Genetic Linkage
Heterozygote Detection
Humans
Lod Score
Male
Middle Aged
Mutation
Neurofibrillary Tangles - pathology
Organ Specificity
Sweden
Abstract
Since the report of a double mutation at codons 670 and 671 of the amyloid precursor protein (APP) gene identified in two Swedish families with clinically diagnosed Alzheimer's disease (AD), a carrier with dementia has died. Neuropathology confirmed the clinical diagnosis of AD. Genealogical investigations have confirmed that the two families are related to common founders. Two-point linkage analysis of the mutation versus the disease in the revised pedigree now gives a lod score of 7.62.
PubMed ID
8028788 View in PubMed
Less detail

An animal model for therapeutic intervention studies of CMV infection in the immunocompromised host.

https://arctichealth.org/en/permalink/ahliterature57796
Source
Arch Virol. 1990;114(1-2):91-107
Publication Type
Article
Date
1990
Author
F S Stals
F. Bosman
C P van Boven
C A Bruggeman
Author Affiliation
Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands.
Source
Arch Virol. 1990;114(1-2):91-107
Date
1990
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Viral - administration & dosage
Antigens, Viral - analysis
Cytomegalovirus Infections - immunology - pathology - therapy
DNA Probes
Disease Models, Animal
Genes, Viral
Immune Tolerance
Immunization, Passive
Male
Nucleic Acid Hybridization
Organ Specificity
Rats
Specific Pathogen-Free Organisms
Abstract
An experimental rat model to study acute cytomegalovirus infections is described. Eight-week old male Brown Norway rats, immunosuppressed by total body irradiation, were infected with rat cytomegalovirus (RCMV). The effects of infection were determined by survival rates and the presence of virus or viral components in different organs was assayed by plaque test, immunoperoxidase staining, dot-blot DNA hybridization and in situ DNA hybridization. At days 10-post infection nearly 90% of the animals had died. Spleen, liver and bone marrow were heavily infected. Interstitial pneumonia was observed. Pathological findings strongly resembled the full scale of lesions in human CMV infections. Anti-RCMV hyperimmune serum was effective against mortality from RCMV infection and viral spread to lungs and liver was prevented. This model is appropriate for studies on the pathogenesis and antiviral therapy of CMV infections in the immunocompromised host.
PubMed ID
2171466 View in PubMed
Less detail

Bacteraemia with an unknown focus: is the focus de facto absent or merely unreported? A one-year hospital-based cohort study.

https://arctichealth.org/en/permalink/ahliterature101748
Source
APMIS. 2011 Apr;119(4-5):275-9
Publication Type
Article
Date
Apr-2011
Author
Inge Kristine Larsen
Gitte Pedersen
Henrik C Schønheyder
Author Affiliation
Department of Infectious Diseases, Aalborg Hospital, Aarhus University Hospital, Denmark.
Source
APMIS. 2011 Apr;119(4-5):275-9
Date
Apr-2011
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Aged
Aged, 80 and over
Bacteremia - diagnosis - etiology - mortality
Bacterial Infections - diagnosis
Child
Child, Preschool
Cohort Studies
Databases, Factual
Denmark - epidemiology
Female
Hospitals
Humans
Infant
Infant, Newborn
Male
Middle Aged
Neutropenia - complications
Organ Specificity
Retrospective Studies
Risk factors
Young Adult
Abstract
An unknown focus of infection is associated with an increased risk of death in patients with bacteraemia. However, the implications for patient management remain uncertain, and to our knowledge, the validity of an unknown focus has not been evaluated. Therefore, we conducted a retrospective record review of bacteraemias with an unknown focus recorded in a regional bacteraemia database. The study cohort comprised 645 cases of bacteraemia diagnosed in 537 hospitalized patients at Aalborg Hospital, Denmark, in 2003. The focus was unknown in 184 (29%) bacteraemia episodes (162 patients). The record review pointed conclusively to a focus in 39 episodes. The positive predictive value of an unknown focus was 79% and the proportion of bacteraemias with a focus increased from 71% to 78%. Among the 145 cases of bacteraemia with a de facto unknown focus, there were 36 incidents of febrile neutropenia and 20 additional incidents of early death which precluded a search for a focus. The study confirmed the focus to be de facto absent in most patients classified with an unknown focus. The distribution of foci changed only marginally by the disclosure of a focus in one of five patients in the 'unknown' group.
PubMed ID
21492227 View in PubMed
Less detail

Bacterial and fungal flora of wild northern fur seals (Callorhinus ursinus).

https://arctichealth.org/en/permalink/ahliterature6979
Source
J Wildl Dis. 1982 Oct;18(4):447-56
Publication Type
Article
Date
Oct-1982
Author
N A Vedros
J. Quinlivan
R. Cranford
Source
J Wildl Dis. 1982 Oct;18(4):447-56
Date
Oct-1982
Language
English
Publication Type
Article
Keywords
Age Factors
Alaska
Animals
Bacteria - isolation & purification
Blood - microbiology
California
Female
Fungi - isolation & purification
Fur Seals - microbiology
Male
Organ Specificity
Pinnipedia - microbiology
Research Support, U.S. Gov't, Non-P.H.S.
Sex Factors
Species Specificity
Abstract
Tissues from healthy subadult and moribund newborn northern fur seals (Callorhinus ursinus) on St. Paul Island, Pribilof Islands, Alaska, and from healthy pups and yearlings on San Miguel Island, California, were sampled for bacteria and fungi. Corynebacterium spp. and Staphylococcus spp. were more frequently present in tissues from animals on St. Paul Island whereas Pseudomonas spp. were frequently isolated on San Miguel Island. Approximately half of the blood samples were positive for bacteria. Salmonella spp. were isolated from rectal swabs of animals only on San Miguel Island. Fungi were isolated from the hair and skin of subadult males.
PubMed ID
7154218 View in PubMed
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101 records – page 1 of 11.