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Characterisation of streptomycin resistance determinants in Danish isolates of Salmonella Typhimurium.

https://arctichealth.org/en/permalink/ahliterature10433
Source
Vet Microbiol. 2000 Jul 3;75(1):73-82
Publication Type
Article
Date
Jul-3-2000
Author
L. Madsen
F M Aarestrup
J E Olsen
Author Affiliation
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigboejlen 4, DK 1870 C, Frederiksberg, Denmark.
Source
Vet Microbiol. 2000 Jul 3;75(1):73-82
Date
Jul-3-2000
Language
English
Publication Type
Article
Keywords
Animals
Blotting, Southern - veterinary
Cattle
Cattle Diseases - drug therapy - microbiology
Colony Count, Microbial
Conjugation, Genetic - genetics
DNA Primers - chemistry
DNA, Bacterial - chemistry - isolation & purification
Denmark
Drug Resistance, Microbial - genetics
Electrophoresis, Agar Gel - veterinary
Humans
Nucleotidyltransferases - chemistry - genetics
Phosphotransferases (Alcohol Group Acceptor) - chemistry - genetics
Polymerase Chain Reaction - veterinary
Research Support, Non-U.S. Gov't
Salmonella Infections, Animal - drug therapy
Salmonella typhimurium - chemistry - drug effects - genetics
Sequence Analysis, DNA
Streptomycin - pharmacology
Swine
Swine Diseases - drug therapy - microbiology
Variation (Genetics) - genetics
Abstract
Fifty six Danish streptomycin (Sm) resistant isolates of Salmonella enterica serotype Typhimurium from pigs (n=34), calves (n=3) and humans (n=19) were characterised with respect to co-resistances (14 drugs), transferability of Sm-resistance by conjugation, genetic determinants encoding Sm-resistance and diversity with respect to localisation of genes in the genome and DNA-sequences. Forty-six strains carried resistance(s) other than Sm-resistance. Nineteen different co-resistance patterns were observed and tetracycline was the most commonly observed resistance in these patterns. In 22 of the strains, Sm-resistance was transferred by conjugation. Eleven strains contained the gene aadA only, six strains contained aadA+strA+strB, and 35 strains contained strA+strB. Partial sequences of aadA were obtained from four strains. Three strains showed identical sequences to a published aadA sequence from the transposon Tn7, and in one strain the sequence showed one synonymous substitution compared to this sequence. Partial sequences were obtained of strA and strB in seven strains. The sequence of strB was identical to the published sequence of the plasmid RSF1010 in all strains. All seven sequences of strA were identical and differed from the sequence of strA in RSF1010 by two non-synonymous substitutions.
PubMed ID
10865153 View in PubMed
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