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Complex chromosome aberrations persist in individuals many years after occupational exposure to densely ionizing radiation: an mFISH study.

https://arctichealth.org/en/permalink/ahliterature174652
Source
Genes Chromosomes Cancer. 2005 Sep;44(1):1-9
Publication Type
Article
Date
Sep-2005
Author
M Prakash Hande
Tamara V Azizova
Ludmilla E Burak
Valentin F Khokhryakov
Charles R Geard
David J Brenner
Author Affiliation
Center for Radiological Research, Columbia University Medical Center, New York, USA.
Source
Genes Chromosomes Cancer. 2005 Sep;44(1):1-9
Date
Sep-2005
Language
English
Publication Type
Article
Keywords
Chromosome Aberrations - radiation effects
Chromosomes, Human - radiation effects
Humans
In Situ Hybridization, Fluorescence
Nuclear Reactors
Occupational Exposure
Plutonium
Radiation, Ionizing
Radon
Russia
Translocation, Genetic
Abstract
Long-lived, sensitive, and specific biomarkers of particular mutagenic agents are much sought after and potentially have broad applications in the fields of cancer biology, epidemiology, and prevention. Many clastogens induce a spectrum of chromosome aberrations, and some of them can be exploited as biomarkers of exposure. Densely ionizing radiation, for example, alpha particle radiation (from radon or plutonium) and neutron radiation, preferentially induces complex chromosome aberrations, which can be detected by the 24-color multifluor fluorescence in situ hybridization (mFISH) technique. We report the detection and quantification of stable complex chromosome aberrations in lymphocytes of healthy former nuclear-weapons workers, who were exposed many years ago to plutonium, gamma rays, or both, at the Mayak weapons complex in Russia. We analyzed peripheral-blood lymphocytes from these individuals for the presence of persistent complex chromosome aberrations. A significantly elevated frequency of complex chromosome translocations was detected in the highly exposed plutonium workers but not in the group exposed only to high doses of gamma radiation. No such differences were found for simple chromosomal aberrations. The results suggest that stable complex chromosomal translocations represent a long-lived, quantitative, low-background biomarker of densely ionizing radiation for human populations exposed many years ago.
PubMed ID
15912529 View in PubMed
Less detail

[Cytochemical lymphocytic markers as criteria suitable for mass and selective hematologic screening of children living in areas contaminated by radionuclides]

https://arctichealth.org/en/permalink/ahliterature36141
Source
Gematol Transfuziol. 1993 Sep-Oct;38(8):34-6
Publication Type
Article
Author
R V Lenskaia
V M Buiankin
G A Zakharova
O A Ikonnikova
M G Koliaskina
O A Popova
A G Rumiantsev
V A Seleznev
I A Sterligova
V M Chernov
Source
Gematol Transfuziol. 1993 Sep-Oct;38(8):34-6
Language
Russian
Publication Type
Article
Keywords
Accidents
Adolescent
Biological Markers - blood
Child
English Abstract
Environmental health
Hematologic Tests
Humans
Lymphocytes - radiation effects
Mass Screening - methods
Nuclear Reactors
Ukraine
Abstract
A cytochemical lymphocytogram by PAS--reaction and nonspecific esterase test was made using blood smears from 820 children living in different regions of the Bryansk area exposed to radioactive contamination and from 46 matched controls from noncontaminated territories. Most of the children were born before the Chernobyl accident. The cytochemical lymphocytogram was informative enough to evaluate lymphocytic pool and its renewal. The lymphocytogram parameters served criteria for selection of children's groups at relative risk. The groups comprised 25-60% of the examinees depending on the contamination degree and were registered for subsequent follow-up and rehabilitation.
PubMed ID
8307305 View in PubMed
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[Health status of children exposed to low doses of radiation 1 year after the Chernobyl AES accident]

https://arctichealth.org/en/permalink/ahliterature37397
Source
Pediatriia. 1991;(12):13-6
Publication Type
Article
Date
1991
Author
I V Koshel'
A G Rumiantsev
Source
Pediatriia. 1991;(12):13-6
Date
1991
Language
Russian
Publication Type
Article
Keywords
Accidents
Adolescent
Age Factors
Child
Child, Preschool
English Abstract
Follow-Up Studies
Humans
Infant
Nuclear Reactors
Radiation Dosage
Radiation Injuries - diagnosis - epidemiology
Time Factors
Ukraine - epidemiology
Abstract
The authors provide data on the health status of children living in radiation polluted regions, obtained 9-11 months after the Chernobyl accident. Study of the demographic characteristics, distribution into health groups and of the hematological parameters did not reveal any significant differences with the exception of a higher anemia incidence (in children under 1 year).
PubMed ID
1788013 View in PubMed
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[Immunophenotype of peripheral blood lymphocytes in children living in radiation-polluted territories]

https://arctichealth.org/en/permalink/ahliterature37896
Source
Pediatriia. 1990;(12):42-7
Publication Type
Article
Date
1990
Author
G I Kovalev
N A Torubarova
A G Rumiantsev
Source
Pediatriia. 1990;(12):42-7
Date
1990
Language
Russian
Publication Type
Article
Keywords
Accidents
Adolescent
Child
Child, Preschool
Comparative Study
English Abstract
Humans
Immunophenotyping
Leukocyte Count
Moscow
Nuclear Reactors
Radiation Dosage
Radiation Injuries - immunology
Russia
Time Factors
Ukraine
Abstract
The authors provide the data on the assessment of the population composition of peripheral blood lymphocytes. The assessment was performed with the aid of antilymphocytic monoclonal antibodies in children populating radioactive cesium polluted territories and exposed to ionizing radiation in small doses. 56 children were examined. They were distributed into 2 groups depending on radiation exposure: 1 year after the breakdown and 3 years after it. For comparison purposes use was made of the data obtained as a result of examination of 39 children from the unpolluted regions. Measurements were made of the T and B lymphocyte counts as well as of the main subpopulations of T cells. The irradiated children manifested no appreciable alterations in cellular immunity pointing to the status of immune deficiency regardless of the duration of radiation exposure. A moderate reduction of the T-lymphocyte count at the expense of T helpers/inducers may be of nonspecific character.
PubMed ID
2150104 View in PubMed
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Past exposure to densely ionizing radiation leaves a unique permanent signature in the genome.

https://arctichealth.org/en/permalink/ahliterature185956
Source
Am J Hum Genet. 2003 May;72(5):1162-70
Publication Type
Article
Date
May-2003
Author
M Prakash Hande
Tamara V Azizova
Charles R Geard
Ludmilla E Burak
Catherine R Mitchell
Valentin F Khokhryakov
Evgeny K Vasilenko
David J Brenner
Author Affiliation
Center for Radiological Research, Columbia University, New York, NY 10032, USA.
Source
Am J Hum Genet. 2003 May;72(5):1162-70
Date
May-2003
Language
English
Publication Type
Article
Keywords
Alpha Particles - adverse effects
Bone Marrow - radiation effects
Chromosome Aberrations
Chromosome Breakage
Chromosome Inversion
Chromosome Painting
Chromosomes, Human - radiation effects - ultrastructure
Chromosomes, Human, Pair 5 - radiation effects - ultrastructure
Gamma Rays - adverse effects
Genome, Human
Humans
In Situ Hybridization, Fluorescence
Inhalation Exposure - adverse effects
Lymphocytes - pathology - radiation effects
Nuclear Reactors
Occupational Exposure - adverse effects
Plutonium - adverse effects
Radiation Dosage
Radiation, Ionizing
Reference Values
Russia
Time
Translocation, Genetic
USSR
Abstract
Speculation has long surrounded the question of whether past exposure to ionizing radiation leaves a unique permanent signature in the genome. Intrachromosomal rearrangements or deletions are produced much more efficiently by densely ionizing radiation than by chemical mutagens, x-rays, or endogenous aging processes. Until recently, such stable intrachromosomal aberrations have been very hard to detect, but a new chromosome band painting technique has made their detection practical. We report the detection and quantification of stable intrachromosomal aberrations in lymphocytes of healthy former nuclear-weapons workers who were exposed to plutonium many years ago. Even many years after occupational exposure, more than half the blood cells of the healthy plutonium workers contain large (>6 Mb) intrachromosomal rearrangements. The yield of these aberrations was highly correlated with plutonium dose to the bone marrow. The control groups contained very few such intrachromosomal aberrations. Quantification of this large-scale chromosomal damage in human populations exposed many years earlier will lead to new insights into the mechanisms and risks of cytogenetic damage.
Notes
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PubMed ID
12679897 View in PubMed
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Revisiting nuclear power plant safety.

https://arctichealth.org/en/permalink/ahliterature18690
Source
Science. 2003 Jan 10;299(5604):201-3; author reply 201-3
Publication Type
Article
Date
Jan-10-2003
Author
David J Brenner
Source
Science. 2003 Jan 10;299(5604):201-3; author reply 201-3
Date
Jan-10-2003
Language
English
Publication Type
Article
Keywords
Accidents, Radiation
Humans
Neoplasms, Radiation-Induced - epidemiology - etiology
Nuclear Energy
Nuclear Reactors
Power Plants
Radiation Dosage
Terrorism
Ukraine - epidemiology
United States
Notes
Comment On: Science. 2002 Sep 20;297(5589):1997-912242425
PubMed ID
12523358 View in PubMed
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Stable intrachromosomal biomarkers of past exposure to densely ionizing radiation in several chromosomes of exposed individuals.

https://arctichealth.org/en/permalink/ahliterature178301
Source
Radiat Res. 2004 Sep;162(3):257-63
Publication Type
Article
Date
Sep-2004
Author
Catherine R Mitchell
Tamara V Azizova
M Prakash Hande
Ludmilla E Burak
Josephine M Tsakok
Valentin F Khokhryakov
Charles R Geard
David J Brenner
Author Affiliation
Center for Radiological Research, Columbia University, New York, New York 10032, USA. cm2073@columbia.edu
Source
Radiat Res. 2004 Sep;162(3):257-63
Date
Sep-2004
Language
English
Publication Type
Article
Keywords
Aged
Aged, 80 and over
Body Burden
Chromosome Aberrations - radiation effects - statistics & numerical data
Chromosome Banding - methods
Chromosomes, Human - radiation effects
Dose-Response Relationship, Radiation
Female
Gamma Rays
Genetic Markers - radiation effects
Humans
Leukocytes - metabolism
Male
Middle Aged
Nuclear Reactors
Occupational Exposure - analysis
Plutonium
Radiation Dosage
Radiation, Ionizing
Radiometry - methods
Risk Assessment - methods
Risk factors
Russia - epidemiology
Abstract
A multicolor banding (mBAND) fluorescence in situ hybridization technique was used to investigate the presence inhuman populations of a stable biomarker-intrachromosomal chromosome aberrations-of past exposure to high-LET radiation. Peripheral blood lymphocytes were taken from healthy Russian nuclear workers occupationally exposed from 1949 onward to either plutonium, gamma rays or both. Metaphase spreads were produced and chromosomes 1 and 2 were hybridized with mBAND FISH probes and scored for intra-chromosomal aberrations. A large yield of intrachromosomal aberrations was observed in both chromosomes of the individuals exposed to high doses of plutonium, whereas there was no significant increase over the (low) background control rate in the population who were exposed to high doses of gamma rays. Interchromosome aberration yields were similar in both the high plutonium and the high gamma-ray groups. These results for chromosome 1 and 2 confirm and extend data published previously for chromosome 5. Intrachromosomal aberrations thus represent a potential biomarker for past exposure to high-LET radiations such as alpha particles and neutrons and could possibly be used as a biodosimeter to estimate both the dose and type of radiation exposure in previously exposed populations.
PubMed ID
15378838 View in PubMed
Less detail

8 records – page 1 of 1.