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[Acute myocardial ischemia-reperfusion injury: role of nitric oxide system]

https://arctichealth.org/en/permalink/ahliterature53337
Source
Fiziol Zh. 2004;50(2):34-42
Publication Type
Article
Date
2004
Author
O O Moibenko
M Ia Iuz'kiv
L V Tumanovs'ka
A V Kotsiuruba
Author Affiliation
A.A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev.
Source
Fiziol Zh. 2004;50(2):34-42
Date
2004
Language
Ukrainian
Publication Type
Article
Keywords
Acute Disease
Animals
Arginine - pharmacology
Coronary Circulation - drug effects - physiology
Coronary Vessels - metabolism
Disease Models, Animal
Dogs
English Abstract
Enzyme Inhibitors - pharmacology
Male
Myocardial Reperfusion Injury - enzymology - metabolism - physiopathology
Nitric Oxide - biosynthesis - physiology
Nitric Oxide Synthase - antagonists & inhibitors
Nitroarginine - pharmacology
Abstract
In experiments on the closed-chest dogs it was shown that NOS inhibition resulted in the significant alterations of hemodynamic indices (coronary and peripheral vascular resistance, cardiac output and heart rate) under local myocardial ischemia/reperfusion in comparison with control experiments. At the first time it was shown that NOS inhibition activated the autophagic destruction of cardiomyocytes in the ischemic myocardium and could reduce an area of functionally active myocardium. L-arginine administration attenuated cardio- and hemodynamic disturbances, that substantially improved the course of ischemia/reperfusion, diminished the ultrastructural changes in myocardium and prevented development of autophagic programmed cell death.
PubMed ID
15174204 View in PubMed
Less detail

[Blocking action of pyridoxal-5'-phosphate on nonadrenergic synaptic inhibition of human small intestine smooth muscle]

https://arctichealth.org/en/permalink/ahliterature29149
Source
Fiziol Zh. 2006;52(1):62-70
Publication Type
Article
Date
2006
Author
O V Romanenko
M M Hrusha
P D Fomin
Source
Fiziol Zh. 2006;52(1):62-70
Date
2006
Language
Ukrainian
Publication Type
Article
Keywords
Adolescent
Adult
Aged
Aged, 80 and over
Child
Child, Preschool
English Abstract
Enzyme Inhibitors - pharmacology
Humans
In Vitro
Infant
Intestinal Diseases - metabolism - physiopathology
Intestine, Small - drug effects - innervation - metabolism
Middle Aged
Muscle, Smooth - drug effects - innervation - metabolism
Nitric Oxide Synthase - antagonists & inhibitors
Nitroarginine - pharmacology
Pyridoxal Phosphate - pharmacology
Synaptic Transmission - drug effects
Abstract
We investigated the inhibitory synaptic potentials (ISP) in isolated smooth muscle strips of the human duodenum circular layer from the ulcer adjacent region (I group) as well as ileum and distal part of small intestine, which were on a distance of some dozen centimeters from the place of disturbance under the different gastrointestinal diseases (II group). ISP amplitude was several times smaller in the muscle strips of the I group compare to the II group. It could depend on the alterations of smooth muscles cable properties, increase of connective tissue mass, changes in the intestinal nervous system and synaptic transmission in the region adjacent to duodenum ulcer. Pyridoxal-5'-phosphate effectively decreased amplitude and increased ISP latent period in the muscle strips from both groups. N(omega)-nitro-L-arginine, the blocker of NO-synthase did not affect pyridoxal-5'-phosphate activity in smooth muscles. Phosphate group was essential for realization of its influence on ISP in smooth muscles because pyridoxal did not influence both ISP amplitude and ISP latent period.
PubMed ID
16553299 View in PubMed
Less detail

[Coronary vascular reaction in non-ischemic myocardium during experimental acute ischemia reperfusion]

https://arctichealth.org/en/permalink/ahliterature53490
Source
Fiziol Zh. 2003;49(4):14-23
Publication Type
Article
Date
2003
Author
O O Moibenko
M Ia Iuz'kiv
V I Azarov
Author Affiliation
A.A. Bogomoletz Institute of Physioligy, Kiev.
Source
Fiziol Zh. 2003;49(4):14-23
Date
2003
Language
Ukrainian
Publication Type
Article
Keywords
Acute Disease
Animals
Coronary Circulation - physiology
Coronary Vessels - physiology
Disease Models, Animal
Dogs
Electrocardiography
English Abstract
Myocardial Reperfusion Injury - metabolism - physiopathology
Myocardium - metabolism
Nitric Oxide - antagonists & inhibitors - biosynthesis
Nitroarginine - pharmacology
Vasodilation - physiology
Abstract
The aim of this study was to investigate coronary vascular responses, particularly NO-dependent, in the non-ischemic miocardium during local acute myocardial ischemia/reperfusion. The experiments were performed on the dogs with closed chest. Occlusion of a branch of the coronary artery resulted in a dilatation of the coronary vessels within the intact part of the myocardium. Neither inhibition of prostanoid production and KATP-channels, nor administration of atropine sulfate and dissection of the vagus nerve altered coronary dilatation within the non-ischemic myocardium. Whereas inhibition of NOS by L-NNA (50 mg/kg) completely changed it after coronary occlusion, furthermore coronary resistance temporally increased. Thus, the most reliable mechanism of that response was NO-dependent.
PubMed ID
14509923 View in PubMed
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Hypothalamic monoamines in cold stress on the background of changes in the activity of the nitric oxide system.

https://arctichealth.org/en/permalink/ahliterature9262
Source
Neurosci Behav Physiol. 2005 Feb;35(2):171-5
Publication Type
Article
Date
Feb-2005
Author
M A Gilinskii
G M Petrakova
T G Amstislavskaya
L N Maslova
V V Bulygina
Author Affiliation
State Science Research Institute of Physiology, Siberian Division, Russian Academy of Medical Sciences, 4 Timakov Street, 630117 Novosibirsk, Russia.
Source
Neurosci Behav Physiol. 2005 Feb;35(2):171-5
Date
Feb-2005
Language
English
Publication Type
Article
Keywords
3,4-Dihydroxyphenylacetic Acid - metabolism
Animals
Biogenic Monoamines - metabolism
Body Temperature - drug effects
Chromatography, High Pressure Liquid - methods
Cold - adverse effects
Comparative Study
Corticosterone - blood
Dopamine - metabolism
Enzyme Inhibitors - pharmacology
Hypothalamus - drug effects - metabolism
Male
Nitric Oxide - physiology
Nitric Oxide Donors - pharmacology
Nitroarginine - pharmacology
Nitroprusside - pharmacology
Norepinephrine - analogs & derivatives - metabolism
Radioimmunoassay - methods
Rats
Rats, Wistar
Stress - metabolism
Time Factors
Abstract
The effects of the NO donor sodium nitroprusside and the NO synthase blocker L-omega-N-nitroarginine (LNA) on body temperature, hypothalamic monoamines, and plasma corticosterone in conditions of cooling were studied in Male Wistar rats. Reductions in body temperature on cooling, both after administration of sodium nitroprusside and LNA, were no different from those seen without treatment. The basal corticosterone level after treatment with sodium nitroprusside increased from 5.3 +/- 2.2 to 29.1 +/- 1.8 microg%. Cooling led to a multiple increase in corticosterone levels in all animals, both in control conditions and after treatment with sodium nitroprusside and LNA. Sodium nitroprusside significantly decreased the basal hypothalamic noradrenaline level, by 37%. Cooling of the animals in these conditions led to an additional drop in the noradrenaline level. Noradrenaline levels 48 h after cold stress applied to animals cooled after treatment with LNA or sodium nitroprusside were significantly higher than in those cooled without treatment. No changes in serotonin and 5-hydroxyindoleacetic acid levels were seen in these experiments. The basal dihydroxyphenylacetic acid and dopamine levels increased after treatment with sodium nitroprusside, by 379% and 239% respectively. No dopamine response to cold was observed, though the dihydroxyphenylacetic acid level in the control group and animals treated with LNA increased. Thus, cold stress did not reveal differently directed directions for the actions of the NO donor and the NO synthase blocker, as seen with other types of stress.
PubMed ID
15779330 View in PubMed
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Nitric oxide as a second messenger in phagocytosis by cultured retinal pigment epithelial cells.

https://arctichealth.org/en/permalink/ahliterature50876
Source
Ophthalmic Res. 2000 Jul-Aug;32(4):138-42
Publication Type
Article
Author
J I Kogishi
M. Akimoto
M. Mandai
S. Kuriyama
M O Hall
Y. Honda
N. Yoshimura
Author Affiliation
Department of Ophthalmology and Visual Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Source
Ophthalmic Res. 2000 Jul-Aug;32(4):138-42
Language
English
Publication Type
Article
Keywords
Animals
Cells, Cultured
Comparative Study
Cyclic GMP - metabolism
Enzyme Inhibitors - pharmacology
Molsidomine - analogs & derivatives - pharmacology
Nitric Oxide - physiology
Nitric Oxide Donors - pharmacology
Nitric Oxide Synthase - antagonists & inhibitors - metabolism
Nitric Oxide Synthase Type III
Nitroarginine - pharmacology
Phagocytosis - drug effects - physiology
Pigment Epithelium of Eye - cytology - metabolism
Rats
Rats, Inbred BN
Rod Outer Segments - physiology
Second Messenger Systems - physiology
Abstract
PURPOSE: To investigate a possible role of the nitric oxide (NO)-cGMP signal transduction system in phagocytosis of rod outer segments (ROS) by cultured retinal pigment epithelial (RPE) cells. METHODS: Primary cultures of RPE cells from 10-day-old Brown Norway rats were used to study the phagocytosis of ROS by these cells. Phagocytosis of ROS was evaluated with or without an inhibitor of nitric oxide synthase (NOS), N(G)-nitro-L-arginine (L-NNA), and the reverse effects of L-NNA by L-arginine and 8-bromo-cGMP on phagocytosis were also studied. NO-associated cGMP production by RPE cells was monitored during phagocytosis using L-NNA. NOS activity was assayed in RPE cells and ROS to locate the source of NO. RESULTS: Phagocytosis of ROS was inhibited by L-NNA but not by D-NNA. L-NNA inhibited the ingestion in a dose-dependent manner, but not the binding of ROS. The inhibition was reversed by L-arginine and also by an NO donor, SIN-1. RPE cells challenged with ROS showed increased cGMP activity, which was significantly reduced by L-NNA and again restored by an overdose of L-arginine. NOS activity was found in RPE cells but not in ROS. CONCLUSIONS: Our data show that cGMP plays a role in the ingestion phase of ROS phagocytosis by RPE cells via a cGMP second-messenger system.
PubMed ID
10828733 View in PubMed
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[Searching for ways to correct thymic dysfunction in rats with experimental diabetes mellitus]

https://arctichealth.org/en/permalink/ahliterature92217
Source
Fiziol Zh. 2008;54(3):28-35
Publication Type
Article
Date
2008
Author
Kolesnyk Iu M
Kamyshnyi O M
Abramov A V
Source
Fiziol Zh. 2008;54(3):28-35
Date
2008
Language
Ukrainian
Publication Type
Article
Keywords
Animals
Apoptosis - drug effects
Arginine - pharmacology
Cell Proliferation - drug effects
Diabetes Mellitus, Experimental - immunology - metabolism - pathology - physiopathology
Enzyme Inhibitors - pharmacology
Insulin - biosynthesis
Male
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - antagonists & inhibitors
Nitroarginine - pharmacology
Proto-Oncogene Proteins c-bcl-2 - metabolism
Rats
Rats, Wistar
T-Lymphocytes - cytology - drug effects - metabolism
Thymus Gland - drug effects - immunology - metabolism - pathology - physiopathology
Abstract
We investigated the influence of multiple introductions of NO precursor L-arginine and NOS non-selective blocker N-nitroL-arginine (NNLA) on thymic morpho-functional status in Wistar male rats with experimental diabetes mellitus (EDM). To reveal insulin-expressing, proliferating, Treg-cells, iNOS(+)-cells and Bcl-2(+)-cells, the immunohistochemical methods of direct and indirect immunofluorescence with monoclonal antibodies to insulin, PCNA, CD-25 antigen. Bcl-2 and iNOS of rat were used. It was established that NNLA administration to rats with EDM has more pronounced effect in comparison with L-arginine administration, demonstrating an increase in the number of Treg-cells, insulin-expressing and proliferating thymocytes and a decrease in the density of iNOS(+)- and Bcl-2(+)-cells population.
PubMed ID
18763577 View in PubMed
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6 records – page 1 of 1.