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Genetic diversity of Neisseria gonorrhoeae multi-antigen sequence types in Russia and Europe.

https://arctichealth.org/en/permalink/ahliterature307179
Source
Int J Infect Dis. 2020 Apr; 93:1-8
Publication Type
Journal Article
Date
Apr-2020
Author
Boris Shaskolskiy
Ekaterina Dementieva
Ilya Kandinov
Alexander Chestkov
Alexey Kubanov
Dmitry Deryabin
Dmitry Gryadunov
Author Affiliation
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. Electronic address: b.shaskolskiy@biochip.ru.
Source
Int J Infect Dis. 2020 Apr; 93:1-8
Date
Apr-2020
Language
English
Publication Type
Journal Article
Keywords
Antigens, Bacterial - genetics
Bacterial Typing Techniques
Europe
Genetic Variation
Genotype
Humans
Neisseria gonorrhoeae - classification - genetics - isolation & purification
Phylogeny
Russia
Abstract
The goal of this work was to assess the genetic diversity of Neisseria gonorrhoeae isolates in Russia and Europe and to compare the distribution of the N. gonorrhoeae multi-antigen sequencing types (NG-MAST) of Russian isolates with that of isolates from European countries.
NG-MAST typing was performed for 804 N. gonorrhoeae isolates collected in Russia in 2013-2018. For isolates from European countries, data from the https://pathogen.watch/collection/eurogasp2013 database were used.
Among the isolates from Russia, 296 NG-MAST types were found. A maximum likelihood phylogenetic tree was constructed. Phylogenetic analysis revealed seven major genogroups uniting the most frequent Russian sequence types: G807, G1993, G9476, G14942, G1152, G9486, and G12531.
The NG-MAST type distribution in Russia differed from that in European countries. Most of the Russian isolates had sequence types that were not found in Europe. Only 33% of the Russian isolates belonged to genogroups established for European countries, and the widespread European genogroup G1407 was represented by only nine isolates. Analysis of the Russian isolates belonging to phylogenetically close European genogroups indicated similarities in drug resistance, although no epidemically dangerous drug-resistant clones were found among the Russian isolates.
PubMed ID
31978578 View in PubMed
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Molecular surveillance of clinical Neisseria gonorrhoeae isolates in Russia.

https://arctichealth.org/en/permalink/ahliterature141919
Source
J Clin Microbiol. 2010 Oct;48(10):3681-9
Publication Type
Article
Date
Oct-2010
Author
Elena N Ilina
Nina Y Oparina
Egor A Shitikov
Alexandra D Borovskaya
Vadim M Govorun
Author Affiliation
Research Institute of Physicochemical Medicine, Ministry of Public Health of Russian Federation, Moscow, Russia. ilinaen@gmail.com
Source
J Clin Microbiol. 2010 Oct;48(10):3681-9
Date
Oct-2010
Language
English
Publication Type
Article
Keywords
Bacterial Typing Techniques - methods
DNA Fingerprinting - methods
DNA, Bacterial - chemistry - genetics
Gonorrhea - epidemiology - microbiology
Humans
Molecular Epidemiology - methods
Molecular Sequence Data
Neisseria gonorrhoeae - classification - genetics - isolation & purification
Porins - genetics
Russia - epidemiology
Sequence Analysis, DNA
Serotyping - methods
Transferrin-Binding Protein B - genetics
Abstract
The choice of adequate methods for epidemiological purposes remains a challenging problem in Neisseria gonorrhoeae molecular monitoring. In this study, the collection of geographically unrelated gonococci (n = 103) isolated in Russian clinics was comparably tested by (i) a traditional serotyping scheme, (ii) por typing, (iii) Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST), and (iv) multilocus sequence typing (MLST). It is shown that, according to sequencing data, a third of the strains carried new porB1 alleles, as well as tbpB ones, and more than half of the samples had new sequence types (STs) as determined by NG-MAST or MLST. The discriminatory power for each typing method was calculated by using the Hunter-Gaston discriminatory index, D. Commonly, modern nucleic acid-based typing methods (por typing, NG-MAST, and MLST) appeared to be more efficient than the classical serotyping scheme. While the traditional serotyping gave a D value of 0.82, the por typing, NG-MAST, and MLST approaches yielded D values of 0.97, 0.98, and 0.91, respectively. Each typing technique revealed the distribution of gonococci slightly correlated with their geographical sources. However, only the MLST method STs were highly associated with certain phenotypes. Although ST1594, ST1892, and ST6720 were typical for susceptible gonococci, ST1901 and ST6716 were undoubtedly associated with a multidrug-resistant phenotype. We conclude that every tested nucleic acid-based typing method is suitable for N. gonorrhoeae molecular surveillance. However, the MLST method seems to serve large-scale epidemiological purposes, whereas the NG-MAST and por typing approaches are more appropriate for the investigation of local outbreaks.
Notes
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PubMed ID
20660213 View in PubMed
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Multiple-locus variable number tandem repeat analysis of Neisseria gonorrhoeae isolates in Russia.

https://arctichealth.org/en/permalink/ahliterature117881
Source
Infect Genet Evol. 2013 Mar;14:8-14
Publication Type
Article
Date
Mar-2013
Author
Anastasiya V Kushnir
Elena N Ilina
Maja V Malakhova
Tatiana V Priputnevich
Maxim L Filipenko
Author Affiliation
Group of Pharmacogenomics, Institute of Chemical Biology and Fundamental Medicine, Lavrentjeva, 8, Novosibirsk 630090, Russia. avenort@gmail.com
Source
Infect Genet Evol. 2013 Mar;14:8-14
Date
Mar-2013
Language
English
Publication Type
Article
Keywords
Anti-Bacterial Agents
Cluster analysis
Drug Resistance, Bacterial - genetics
Genetic Loci
Genotype
Gonorrhea - epidemiology
Humans
Microbial Sensitivity Tests
Minisatellite Repeats - genetics
Multilocus Sequence Typing - methods
Neisseria gonorrhoeae - classification - genetics - isolation & purification
Russia
Abstract
In the present study, a multiple-locus variable number tandem repeat analysis (MLVA) was used to assess the molecular epidemiology of Neisseria gonorrhoeae clinical isolates originating from different regions of Russia. MLVA, based on seven loci, was performed on 218 isolates that were previously tested for susceptibility to penicillin, tetracycline and ciprofloxacin and for the presence of certain genetic determinants of drug resistance. In total, 83 MLVA types were identified, indicating that MLVA is a highly discriminatory technique with a Hunter-Gaston discriminatory index of 0.963 (95% CI, 0.950-0.977). MLVA type 16 was shown to be the most prevalent type and is undoubtedly associated with a multidrug resistant phenotype. The spread of MLVA type 16 from Moscow to Irkutsk suggests that this type has a highly successful transmission rate. Hierarchical cluster analysis of the MLVA profiles classified 208 isolates (95%) into six large groups (containing more than 10 isolates). Clusters differed in geographical characteristics and susceptibility profiles. MLVA cluster A comprised in total 34 isolates and was unambiguously associated with multidrug resistance. Most isolates in cluster A carried mutations in penA, ponA, rpsJ, mtrR, gyrA, and parC genes. MLVA cluster D was associated with resistance to penicillin and with mutations in ponA and rpsJ genes and the presence of plasmid-borne bla(TEM-1) gene. MLVA clusters B, C and E were associated with susceptibility to ciprofloxacin and had a lack of mutations in ponA, rpsJ, gyrA, and parC genes. We conclude that MLVA will be a useful tool for N. gonorrhoeae epidemiological studies.
PubMed ID
23257414 View in PubMed
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Neisseria gonorrhoeae. Surveillance of penicillinase-producing strains.

https://arctichealth.org/en/permalink/ahliterature228887
Source
Wkly Epidemiol Rec. 1990 Jun 8;65(23):176-7
Publication Type
Article
Date
Jun-8-1990

A trial of the validity of genital smears and cultures with gonococcal vaccine provocation in diagnosing genital gonorrhoea in women.

https://arctichealth.org/en/permalink/ahliterature153534
Source
Int J STD AIDS. 2009 Jan;20(1):24-9
Publication Type
Article
Date
Jan-2009
Author
A. Renton
E. Filatova
C. Ison
A. Meheus
G. Dmitriev
V. Akovbian
D. Taylor-Robinson
Author Affiliation
Institute for Health and Human Development, University of East London, Arthur Edwards Building, Romford Road, London, UK. a.renton@uel.ac.uk
Source
Int J STD AIDS. 2009 Jan;20(1):24-9
Date
Jan-2009
Language
English
Publication Type
Article
Keywords
Bacterial Vaccines - administration & dosage
Cervix Uteri - microbiology
Culture Media
Female
Gonorrhea - diagnosis - microbiology
Humans
Ligase Chain Reaction
Microscopy - methods
Neisseria gonorrhoeae - classification - genetics - isolation & purification
Russia
Sensitivity and specificity
Vaginal Smears
Abstract
In Russia the diagnosis of gonorrhoea in women relied on microscopy, justified by the hypothesis that sensitivity increases using 'provocation' techniques. The aim was to test the value of Gonovaccine as provocation in women who would have received it normally. Cervical specimens from 204 women were tested by culture and a ligase chain reaction (LCR) assay before the women were randomized to receive provocation or not. Further cervical specimens were obtained 24, 48 and 72 hours later for microscopy, culture and LCR tests. In both provocation and non-provocation arms, 24 women were positive for gonorrhoea by the LCR assay. Test-by-test, sensitivity of microscopy was 30% in the provocation arm and 13% in the control arm (P = 0.0407, Fisher's exact test). Patient-by-patient, sensitivity of microscopy was 50% in the provocation arm, but only 25% in the control arm (P = 0.0675, Fisher's exact test). The cost per case was greater ($214) using provocation with microscopy than culture and microscopy at the first visit ($150). Thus, although Gonovaccine provocation doubled the sensitivity of microscopy in detecting gonococci, the internationally recommended protocol of microscopy and culture at first visit should be adopted as routine practice in Russia. The findings raise questions about the pathogenesis and natural history of gonorrhoea.
Notes
Comment In: Int J STD AIDS. 2009 Apr;20(4):292-419304985
PubMed ID
19103889 View in PubMed
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