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Association of Epstein-Barr virus with nasopharyngeal carcinoma in Alaskan native patients: serum antibodies and tissue EBNA and DNA.

https://arctichealth.org/en/permalink/ahliterature4247
Source
Int J Cancer. 1981 Sep 15;28(3):301-5
Publication Type
Article
Date
Sep-15-1981
Author
A P Lanier
G W Bornkamm
W. Henle
G. Henle
T R Bender
M L Talbot
P H Dohan
Source
Int J Cancer. 1981 Sep 15;28(3):301-5
Date
Sep-15-1981
Language
English
Geographic Location
U.S.
Publication Type
Article
Keywords
Alaska
Antibodies, Viral - analysis
Antigens, Viral - analysis
Asian Continental Ancestry Group
DNA, Viral - analysis
Epstein-Barr Virus Nuclear Antigens
Herpesvirus 4, Human - immunology
Humans
Nasopharyngeal Neoplasms - immunology - microbiology
Nucleic Acid Hybridization
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Salivary Gland Neoplasms - analysis
Abstract
Biopsy specimens from Alaskan Native patients with nasopharyngeal carcinoma (NPC) and from other patients seen on the otolaryngology service were tested for Epstein-Barr virus-specific DNA and nuclear antigen (EBNA). Serum samples from both groups were tested for various EBV-related antibodies. EBV DNA and EBNA results were in agreement in 29 of 31 tissue specimens tested by the two methods. Ten of 11 biopsies containing NPC cells were positive for EBV DNA. Two NPC patients had biopsies that showed only atypical epithelium but were also positive for EBV DNA or EBNA. The other tissue specimens were negative except for biopsies from two patients: one with a parotid gland lymphoepithelial lesion; another with undifferentiated carcinoma of salivary gland origin.
PubMed ID
6274814 View in PubMed
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Characterization of a murine monoclonal-antibody-defined B-lymphocyte carcinoma cross-reacting antigen (BLCa) from nasopharyngeal carcinoma tissues.

https://arctichealth.org/en/permalink/ahliterature4189
Source
Int J Cancer. 1987 Apr 15;39(4):449-51
Publication Type
Article
Date
Apr-15-1987
Author
T C Yip
K H Chan
D. Choy
C W Chan
M H Ng
Source
Int J Cancer. 1987 Apr 15;39(4):449-51
Date
Apr-15-1987
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Monoclonal - immunology
Antigens, Neoplasm - analysis
Antigens, Surface - analysis
B-Lymphocytes - immunology
Carcinoma - immunology
Cross Reactions
Humans
Mice
Nasopharyngeal Neoplasms - immunology
Research Support, Non-U.S. Gov't
Abstract
Biopsies were obtained from 12 patients suspected of having nasopharyngeal carcinoma (NPC). A portion of the tissue was submitted for histopathology and another for Western blotting using a murine monoclonal antibody (MAb), MA6. Touch smears of the tissues were also prepared immediately prior to extraction and Western blotting for immunoenzymic staining. The results showed that a B-lymphocyte carcinoma cross-reacting antigen (BLCa), or an antigen similar to it, was the major antigen in the tumor tissue recognized by MA6. The antigen was detected in tissues from 8 patients, of whom 7 had confirmed NPC and one had eskimoma, but not in tissue from the remaining 4 patients who did not have histologically confirmed malignancy. Immunocytology showed that tumour cells were present in the touch smears from all but one of the tumour patients but not in the other patients, and that the tumour cells comprised the large majority of the MA6-reactive cell population. The other MA6-reactive cell types present included certain weakly reactive epithelial cells and occasional lymphoid cells, presumably B lymphocytes. However, these cell types were similarly distributed between the tissues obtained from patients with or without malignant diseases. It was concluded, therefore, that the tumour cells in these tissues are the principal source of BLCa and, as such, the antigen may constitute an objective and reliable marker of NPC.
PubMed ID
3493980 View in PubMed
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Complement-fixing antibody to Epstein-Barr virus soluble antigen in populations at high and low risk for nasopharyngeal carcinoma.

https://arctichealth.org/en/permalink/ahliterature4237
Source
Int J Cancer. 1982 Mar 15;29(3):265-8
Publication Type
Article
Date
Mar-15-1982
Author
P H Levine
P. Ebbesen
R R Connelly
S. Das
M. Middleton
M. Mestre
Source
Int J Cancer. 1982 Mar 15;29(3):265-8
Date
Mar-15-1982
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Age Factors
Antibodies, Viral - analysis
Antigens, Viral - immunology
Capsid - immunology
Complement Fixation Tests
Denmark
Environment
Female
Greenland
Herpesvirus 4, Human - immunology
Humans
Inuits
Male
Middle Aged
Nasopharyngeal Neoplasms - immunology
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Sex Factors
Abstract
Greenland Eskimos comprise an ethnic group with one of the highest recorded incidence rates for nasopharyngeal carcinoma in the world. Sera from 625 Eskimos and 73 Danes (Caucasians) living in Greenland, as well as from 62 Danes living in Denmark, were tested for complement-fixing antibody to Epstein-Barr virus (EBV) soluble antigen and, from this study group, 129 donors were matched by age and sex for a study comparing antibody to viral capsid antigen, early antigen, and soluble antigen. Both Eskimos and Danes living in Greenland had significantly higher titers of EBV antibodies than Danes living in Denmark, suggesting that environment was more important than genetics or socio-economic factors in determining the antibody response to EBV. Age and sex were also factors, higher titers occurring in females and young Eskimos.
PubMed ID
6279526 View in PubMed
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Evaluation of monoclonal antibodies for the detection of exfoliative nasopharyngeal carcinoma cells.

https://arctichealth.org/en/permalink/ahliterature4188
Source
Int J Cancer. 1987 Apr 15;39(4):445-8
Publication Type
Article
Date
Apr-15-1987
Author
K H Chan
T C Yip
D. Choy
C W Chan
Y. Zeng
M H Ng
Source
Int J Cancer. 1987 Apr 15;39(4):445-8
Date
Apr-15-1987
Language
English
Publication Type
Article
Keywords
Adult
Aged
Antibodies, Monoclonal - diagnostic use
Carcinoembryonic Antigen - analysis
Carcinoma - immunology - pathology
Female
Humans
Male
Middle Aged
Nasopharyngeal Neoplasms - immunology - pathology
Research Support, Non-U.S. Gov't
Abstract
Exfoliative cells were aspirated from 15 patients suspected of having nasopharyngeal carcinoma (NPC) and showing the presence of lesions or other abnormalities in the nasopharynx. They were tested for binding with a 125I monoclonal antibody (MAb) (MA6) which is selectively reactive against human B lymphocytes and a variety of carcinomas. A positive result was obtained from 6/9 patients with, and from 0/5 patients without, histologically confirmed disease. One patient with eskimoma also gave a negative binding result. Cytology was specific but less sensitive, tumour cells being detected in 3 of the patients with confirmed disease. Immunocytology using MA6 was limited, like cytology, by poor recovery of the tumour cells and the results were in complete concordance with cytology. The other MAbs used were raised against carcinoembryonic antigen (CEA) and a carcinoma cell line (Ca2), respectively. The latter was not reactive against the NPC tumour cells while the CEA antibody was not sufficiently selective to be useful.
PubMed ID
3557704 View in PubMed
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[The human herpesvirus type 6 (HHV-6) and its spread in Russia].

https://arctichealth.org/en/permalink/ahliterature221625
Source
Vopr Virusol. 1993 Mar-Apr;38(2):66-9
Publication Type
Article
Author
V N Stepina
T A Afanas'eva
V V Evtushenko
A V Pivnik
M M Riashentsev
Iu N Andreev
Kh Kistoglu
V E Gurtsevich
Source
Vopr Virusol. 1993 Mar-Apr;38(2):66-9
Language
Russian
Publication Type
Article
Keywords
Antibodies, Viral - blood
Antibody Specificity
Arthritis, Rheumatoid - immunology
Blood Donors - statistics & numerical data
Hemophilia A - immunology
Herpesviridae Infections - epidemiology - immunology - microbiology
Herpesvirus 6, Human - immunology - isolation & purification
Humans
Kidney Transplantation - immunology
Leukemia - immunology
Nasopharyngeal Neoplasms - immunology
Prevalence
Russia - epidemiology
Seroepidemiologic Studies
Abstract
This is the first presentation of information on the circulation of human herpes virus type 6 (HHV-6) in the population of the mid-European part of this country. The results of the study indicate that HHV-6 is widely spread both among apparently normal people and among patients with different pathologies. The studies using immunofluorescence test showed that the percentage of HHV-6-infected subjects was practically similar among blood donors, patients with nasopharyngeal cancer, rheumatoid arthritis, hemophilia, and patients with transplanted kidney, varying from 34% to 49%. A significantly higher per cent (66%) and higher antibody titres to HHV-6 were detected in sera from patients with hemoblastoses. A high level of HHV-6 infection among patients with hemoblastoses was also confirmed by the results of immunoblotting showing a wider spectrum and higher intensity of the detectable proteins. It should be noted that the amounts of virus-specific proteins detectable by some sera showed individual variations indicating different levels of expression of viral proteins in different individuals.
PubMed ID
8059523 View in PubMed
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6 records – page 1 of 1.