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TDP-1, a toxic component causing tibial dyschondroplasia in broiler chickens, and trichothecenes from Fusarium roseum 'Graminearum'.

https://arctichealth.org/en/permalink/ahliterature75650
Source
Appl Environ Microbiol. 1985 Jul;50(1):102-7
Publication Type
Article
Date
Jul-1985
Author
Y W Lee
C J Mirocha
D J Shroeder
M M Walser
Source
Appl Environ Microbiol. 1985 Jul;50(1):102-7
Date
Jul-1985
Language
English
Publication Type
Article
Keywords
Alaska
Animal Feed
Animals
Cereals - microbiology
Chick Embryo
Chickens
Chromatography, Thin Layer
Fusarium - isolation & purification - metabolism
Mouth Diseases - etiology - veterinary
Mycotoxins - biosynthesis - isolation & purification - poisoning
Osteochondrodysplasias - epidemiology - etiology - veterinary
Poultry Diseases - etiology
Research Support, U.S. Gov't, Non-P.H.S.
Sesquiterpenes - poisoning
Spectrum Analysis, Mass
Time Factors
Trichothecenes - biosynthesis - poisoning
Abstract
Fusarium roseum 'Graminearum' was isolated from overwintered oats in Alaska and was tested for its ability to cause tibial dyschondroplasia (TDP) in broiler chickens. The water-soluble fraction was tested and found to cause TDP. In addition, diacetoxyscirpenol and 7-hydroxydiacetoxyscirpenol were identified in the acetonitrile fraction of the extracts and caused mild mouth lesions in chickens. Six major water-soluble components were purified by thin-layer chromatography and tested for toxicity to chick embryos. One of the six components, called TDP-1, was found to be lethal to chick embryos. There was a 100% incidence of TDP in chickens fed a diet containing 75 ppm (wt/wt) of pure TDP-1, thus establishing the cause and effect relationship between TDP and TDP-1. Analyses by thin-layer chromatography and mass spectrometry revealed that TDP-1 is polar and ninhydrin positive, exhibits fluorescence with UV irradiation, and is a nitrogen-containing component with an empirical formula of C15H20N2O4.
PubMed ID
4026285 View in PubMed
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