Skip header and navigation

Refine By

11 records – page 1 of 2.

Alpha-smooth muscle actin within epithelial islands is predictive of ameloblastic carcinoma.

https://arctichealth.org/en/permalink/ahliterature153094
Source
Oral Oncol. 2009 Sep;45(9):760-5
Publication Type
Article
Date
Sep-2009
Author
I O Bello
K. Alanen
P J Slootweg
T. Salo
Author Affiliation
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Finland.
Source
Oral Oncol. 2009 Sep;45(9):760-5
Date
Sep-2009
Language
English
Publication Type
Article
Keywords
Actins - metabolism
Ameloblastoma - metabolism - pathology
Calcium-Binding Proteins - metabolism
Carcinoma - metabolism - pathology
Female
Finland
Flow Cytometry
Humans
Image Cytometry
Ki-67 Antigen - metabolism
Male
Mandibular Neoplasms - metabolism - pathology
Maxillary Neoplasms - metabolism - pathology
Membrane Proteins - metabolism
Microfilament Proteins - metabolism
Mucin-1 - metabolism
Muscle, Smooth - metabolism - pathology
Neoplasm Proteins - metabolism
Netherlands
Retrospective Studies
Abstract
Ameloblastoma is the most common clinically significant odontogenic tumor. It is considered benign but locally invasive and associated with variable clinico-pathological behavior. Ameloblastic carcinoma is a malignant tumor having features of ameloblastoma in addition to cytologic atypia with or without metastasis. It is aggressive and associated with poor prognosis. The aim of this study was to examine which epithelial and stromal markers are predictive of histologically diagnosed ameloblastic carcinoma and can sufficiently differentiate it from solid/multicystic ameloblastoma (SA). We examined immunohistochemically Ki-67, epithelial membrane antigen (EMA), alpha-smooth muscle actin (alpha-SMA), calponin, p63 and DNA content using image (ICM) and flow cytometry (FCM) in three ameloblastic carcinomas and up to 18 SAs. The important findings were that Ki-67 labeling index was significantly higher in ameloblastic carcinoma than SA while EMA, calponin, p63, ICM and FCM did not sufficiently differentiate the two groups of lesions. Expression of alpha-SMA was consistently obtained within the epithelial island cells of ameloblastic carcinoma and not in SA, although the marker was well expressed in the stroma of both lesions. We therefore conclude that the presence of alpha-SMA within the epithelial islands is highly predictive of ameloblastic carcinoma.
PubMed ID
19150605 View in PubMed
Less detail

The epidermal growth factor receptor mediates allergic airway remodelling in the rat.

https://arctichealth.org/en/permalink/ahliterature92724
Source
Eur Respir J. 2008 Nov;32(5):1213-23
Publication Type
Article
Date
Nov-2008
Author
Tamaoka M.
Hassan M.
McGovern T.
Ramos-Barbón D.
Jo T.
Yoshizawa Y.
Tolloczko B.
Hamid Q.
Martin J G
Author Affiliation
Meakins-Christie Laboratories, Dept of Medicine, McGill University, 3626 St Urbain Street, Montreal, QC, H2X 2P2, Canada.
Source
Eur Respir J. 2008 Nov;32(5):1213-23
Date
Nov-2008
Language
English
Publication Type
Article
Keywords
Allergens - metabolism
Animals
Cell Proliferation
Cysteine - chemistry
Gene Expression Profiling
Goblet Cells - pathology
Hyperplasia - pathology
Inflammation
Leukotriene D4 - metabolism
Muscle, Smooth - metabolism
Ovalbumin - metabolism
Rats
Receptor, Epidermal Growth Factor - physiology
Receptors, Leukotriene - metabolism
Tyrphostins - pharmacology
Abstract
The chronicity of bronchial asthma is attributed to persistent airway inflammation and to a variety of structural changes, or remodelling, that includes smooth muscle and goblet cell hyperplasia. To investigate the mechanisms of airway remodelling, the current authors used an established allergen (ovalbumin; OVA)-driven rodent model (the Brown Norway rat). Brown Norway rats were sensitised to OVA and challenged three times at 5-day intervals to evoke airway remodelling. The effects of an epidermal growth factor (EGF) receptor inhibitor, AG1478, and a cysteinyl leukotriene-1 receptor antagonist, montelukast, on epithelial and airway smooth muscle (ASM) cell proliferation in vivo in response to repeated OVA challenge were tested. Three challenges with leukotriene (LT)D(4) were given, to examine their effects on remodelling with and without AG1478 pretreatment. OVA challenges caused ASM hyperplasia, with an increase in mass, epithelial cell proliferation and goblet cell proliferation. AG1478 prevented the changes, as did montelukast. Multiple OVA challenges increased heparin-binding EGF-like growth factor but not EGF expression by airway epithelium. LTD(4) reproduced the changes in remodelling induced by OVA and this was blocked by AG1478. Allergen-induced airway epithelial and airway smooth muscle remodelling is mediated by cysteinyl leukotrienes via the cysteinyl leukotriene-1 receptor with downstream effects on the epidermal growth factor receptor axis.
PubMed ID
18653647 View in PubMed
Less detail

[Interrelation between levels of eicosanoids and tachykinins in the expired air condencate in the obstructive type respiratory insufficiency in patients with destructive pulmonary tuberculosis]

https://arctichealth.org/en/permalink/ahliterature81799
Source
Lik Sprava. 2000 Oct-Dec;(7-8):27-30
Publication Type
Article
Author
Protsiuk R H
Slyvka V I
Shapovalov V P
Source
Lik Sprava. 2000 Oct-Dec;(7-8):27-30
Language
Ukrainian
Publication Type
Article
Keywords
Breath Tests
Bronchi - metabolism
Eicosanoids - analysis - metabolism
Humans
Muscle, Smooth - metabolism
Respiratory Insufficiency - complications - metabolism - physiopathology
Respiratory Mechanics - physiology
Tachykinins - analysis - metabolism
Tuberculosis, Pulmonary - complications - metabolism - physiopathology
Abstract
The content was studied of vasoactive intestinal peptide (VIP), substantia P (SP), bombesine (BMB), prostaglandins (PG) E2, F2alpha, 6-keto-F1alpha, Tx A2, and leukotrien (LT) B4 in the expired air condensate with the aid of the radioimmune technique to reveal a relation between pulmonary tachykinins and eicosanoids in obstructive type respiratory incompetence in patients with pulmonary tuberculosis. In patients with pulmonary tuberculosis, a high level of BMB was measurable in the expired air condensate. A linear negative correlation has been established between BMB content and forced expiratory volume, SP and respiratory volume, SP and lung capacity. In pulmonary tuberculosis, PGE2 and PGF2alpha levels are significantly evaluated. There was a positive correlation between BMB content and PGF2a in the expired air condensate, which fact suggests their synergic action on the smooth muscles of the bronchi and bronchioles.
PubMed ID
16786641 View in PubMed
Less detail

Involvement of cysteinyl leukotrienes in airway smooth muscle cell DNA synthesis after repeated allergen exposure in sensitized Brown Norway rats.

https://arctichealth.org/en/permalink/ahliterature15646
Source
Br J Pharmacol. 1999 Jul;127(5):1151-8
Publication Type
Article
Date
Jul-1999
Author
M. Salmon
D A Walsh
T J Huang
P J Barnes
T B Leonard
D W Hay
K F Chung
Author Affiliation
Thoracic Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London, UK.
Source
Br J Pharmacol. 1999 Jul;127(5):1151-8
Date
Jul-1999
Language
English
Publication Type
Article
Keywords
Acetylcholine - pharmacology
Allergens - immunology
Animals
Arachidonate 5-Lipoxygenase - physiology
Bronchi - drug effects - metabolism
Cell Count
Cysteine - physiology
DNA - biosynthesis
Eosinophils - physiology
Leukotrienes - physiology
Male
Muscle, Smooth - metabolism
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Abstract
Airway smooth muscle thickening is a characteristic feature of airway wall remodelling in chronic asthma. We have investigated the role of the leukotrienes in airway smooth muscle (ASM) and epithelial cell DNA synthesis and ASM thickening following repeated allergen exposure in Brown Norway rats sensitized to ovalbumin. There was a 3 fold increase in ASM cell DNA synthesis, as measured by percentage bromodeoxyuridine (BrdU) incorporation, in repeatedly ovalbumin-exposed (4.1%, 3.6-4.6; mean, 95% c.i.) compared to chronically saline-exposed rats (1.3%, 0.6-2.1; P
PubMed ID
10455261 View in PubMed
Less detail

[Modeling of K+ATP channel activity in normotensive and hypertensive animals]

https://arctichealth.org/en/permalink/ahliterature10199
Source
Fiziol Zh. 2000;46(6):54-60
Publication Type
Article
Date
2000
Author
B. Strutynsk'kyiR
O O Moibenko
Author Affiliation
A.A. Bogomoletz Institute of Physiology, National Academy of Science of Ukraine, Kiev.
Source
Fiziol Zh. 2000;46(6):54-60
Date
2000
Language
Ukrainian
Publication Type
Article
Keywords
Acetylcholine - metabolism
Angiotensin II - metabolism
Animals
Blood Pressure - physiology
English Abstract
Guinea Pigs
Hypertension - metabolism - physiopathology
Muscle, Smooth - metabolism
Norepinephrine - metabolism
Potassium Channels - physiology
Abstract
It was shown that vasodilatory effects of ATP-sensitive potassium channels opener--flocalin--depend from character of increase of initial vessel tone. Norepinephrine and angiotensin II partially inhibit effects of flocalin, thus they depressed of KATP channel activity. The inhibitory effect of angiotensin II increases under arterial hypertension conditions. It was shown that vasodilatory effects of flocalin significantly do not distinguish in experiments on normotensive and hypertensive rats under K(+)-depolarization conditions. While the vasodilatory effects acetylcholine on hypertensive rats are significantly diminished. These data suggests that flocalin could be a perspective substance for treatment of hypertension.
PubMed ID
11424564 View in PubMed
Less detail

Nitric oxide relaxes rat tail artery smooth muscle by cyclic GMP-independent decrease in calcium sensitivity of myofilaments.

https://arctichealth.org/en/permalink/ahliterature9462
Source
Cell Calcium. 2004 Aug;36(2):165-73
Publication Type
Article
Date
Aug-2004
Author
A. Soloviev
V. Lehen'kyi
S. Zelensky
P. Hellstrand
Author Affiliation
Department of Experimental Therapeutics, Institute of Pharmacology and Toxicology, Academy of Medical Sciences, 14 Ezhena Pot'e, 03057 Kiev, Ukraine. s.a.pharm@naverex.kiev.ua
Source
Cell Calcium. 2004 Aug;36(2):165-73
Date
Aug-2004
Language
English
Publication Type
Article
Keywords
Animals
Arteries - metabolism
Calcium - metabolism
Cyclic GMP - metabolism
Membrane Potentials - physiology
Microfilaments - metabolism
Muscle, Smooth - metabolism
Nitric Oxide - metabolism
Nitroglycerin - metabolism
Nitroprusside - metabolism
Rats
Time Factors
Abstract
The effects of authentic nitric oxide (NO, 10(-6) M) and NO-donors such as sodium nitroprusside (SNP, 10(-5) M) and glyceryl trinitrate (GTN, 10(-4) M) on contractile force and free intracellular calcium level ([Ca2+]i) were studied on precontracted with high potassium chloride (KCl, 70 mM) isolated rings of rat tail artery. The sensitivity of contractile myofilaments to Ca2+ was measured using chemically permeabilized (alpha-toxin, beta-escin, Triton X-100) vascular rings. [Ca2+]i and contractile activity were measured simultaneously. The relationship of [Ca2+]i and tension developed was studied in endothelium-denuded rings and controlled calcium response was evaluated in both endothelium-denuded and permeabilized vascular rings. Both authentic NO and NO-donors decreased [Ca2+]i and high potassium-induced tension with a different time course. Inhibitor of soluble guanylyl cyclase (sGC) LY83583 (10(-5) M) did not affect SNP-induced relaxation whereas the other sGC inhibitor ODQ (10(-6) M) attenuated SNP-induced relaxation. Both inhibitors had no effect on NO- and SNP-induced reduction in [Ca2+]i. On the contrary, GTN induced neither relaxation nor decrease in [Ca2+]i on application of both LY83583 and ODQ. Tail artery rings permeabilized with alpha-toxin, beta-escin, but not with Triton X-100 were relaxed by authentic NO and NO-donors, but to a less extent than non-permeabilized rings. Dithioerythritol (DTE, 5 x 10(-3) M) that maintains sulfhydryl (SH) groups in reduced state preventing their nitrosylation attenuated NO-induced relaxation in both non-permeabilized and permeabilized tail artery rings. The cyclic heptapeptide mycrocystin-LR (MC-LR) (10(-5) M), an inhibitor of type 1 and 2A phosphatases, induced sustained increase in tension of beta-escin permeabilized rings in low Ca2+ (10(-8) M) solution. The tension was not affected by authentic NO and SNP. We conclude that authentic NO and SNP relax rat tail artery smooth muscle (SM) in the presence of inhibitors of sGC via cyclic guanosine monophosphate (cGMP)-independent pathway, whereas relaxation induced by GTN is inhibited. The data demonstrate that cGMP-dependent pathway in vascular smooth muscle is ubiquitous, but not the only way of relaxation induced by NO. NO can modulate vascular tone directly by reducing sensitivity of contractile myofilaments to [Ca2+]i and may involve activation of protein phosphatase(s).
PubMed ID
15193864 View in PubMed
Less detail

[Protein composition of the smooth muscle sarcoplasm in the uterus in pregnancy]

https://arctichealth.org/en/permalink/ahliterature66697
Source
Ukr Biokhim Zh. 1970;42(4):445-9
Publication Type
Article
Date
1970

Repeated allergen exposure of sensitized Brown-Norway rats induces airway cell DNA synthesis and remodelling.

https://arctichealth.org/en/permalink/ahliterature15632
Source
Eur Respir J. 1999 Sep;14(3):633-41
Publication Type
Article
Date
Sep-1999
Author
M. Salmon
D A Walsh
H. Koto
P J Barnes
K F Chung
Author Affiliation
Thoracic Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London, UK.
Source
Eur Respir J. 1999 Sep;14(3):633-41
Date
Sep-1999
Language
English
Publication Type
Article
Keywords
Actins - metabolism
Allergens - pharmacology
Animals
Antigens, CD2 - metabolism
Asthma - chemically induced - metabolism - pathology
Blood Proteins - metabolism
Bromodeoxyuridine - metabolism
Bronchial Hyperreactivity - chemically induced - metabolism - pathology
Comparative Study
DNA - biosynthesis
Disease Models, Animal
Eosinophil Granule Proteins
Eosinophils - metabolism - pathology
Epithelial Cells - metabolism - pathology
Lung - drug effects - metabolism - pathology
Male
Muscle, Smooth - metabolism - pathology
Ovalbumin - pharmacology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Ribonucleases
T-Lymphocytes - metabolism - pathology
Abstract
Chronic inflammation in asthmatic airways can lead to characteristic airway smooth muscle (ASM) thickening and pathological changes within the airway wall. This study assessed the effect of repeated allergen exposure on ASM and epithelial cell deoxyribonucleic acid (DNA) synthesis, cell recruitment and airway wall pathology. Brown-Norway rats were sensitized and then exposed to ovalbumin or saline aerosol every 3 days on six occasions. After the final exposure, rats were administered twice daily for 7 days with the DNA S-phase marker bromodeoxyuridine (BrdU). Using a triple immunohistochemical staining technique, BrdU incorporation into ASM and epithelium was quantified employing computer-assisted image analysis. There were >3-fold mean increases in BrdU incorporation into ASM from 1.3% of cells (95% confidence interval (CI) 1.0-1.6) in saline controls to 4.7% (95% CI 2.6-6.7) after allergen exposure (p
PubMed ID
10543287 View in PubMed
Less detail

Segregation of a missense variant in enteric smooth muscle actin ?-2 with autosomal dominant familial visceral myopathy.

https://arctichealth.org/en/permalink/ahliterature120877
Source
Gastroenterology. 2012 Dec;143(6):1482-1491.e3
Publication Type
Article
Date
Dec-2012
Author
Heli J Lehtonen
Taina Sipponen
Sari Tojkander
Riitta Karikoski
Heikki Järvinen
Nigel G Laing
Pekka Lappalainen
Lauri A Aaltonen
Sari Tuupanen
Author Affiliation
Department of Medical Genetics, Genome-Scale Biology Research Program, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland.
Source
Gastroenterology. 2012 Dec;143(6):1482-1491.e3
Date
Dec-2012
Language
English
Publication Type
Article
Keywords
Actins - genetics - metabolism
Adult
Chromosome Segregation - genetics
Exome - genetics
Female
Finland
Gastrointestinal Motility - physiology
Genome-Wide Association Study
Heterozygote
Humans
Intestinal Pseudo-Obstruction - genetics - metabolism - physiopathology
Intestines - metabolism - physiopathology
Male
Middle Aged
Muscle Contraction - physiology
Muscle, Smooth - metabolism - physiopathology
Mutation, Missense - genetics
Myenteric Plexus - abnormalities - metabolism - physiopathology
Pedigree
Phenotype
Abstract
Familial visceral myopathy (FVM) is a rare inherited form of myopathic pseudo-obstruction; little is known about the genetic factors that cause this disorder. FVM is characterized by impaired functions of enteric smooth muscle cells, resulting in abnormal intestinal motility, severe abdominal pain, malnutrition, and even death. We searched for genetic factors that might cause this disorder.
We performed whole-exome sequence analysis of blood samples from 2 individuals in a family that had 7 members diagnosed with FVM. Sanger sequencing was used to analyze additional family members and 280 individuals without this disorder (controls). Intestinal tissue samples from 4 patients and 2 controls were analyzed by immunohistochemistry. Functional studies, including immunofluorescence, cell contractility, and actomyosin structure analyses, were performed using CRL-1976 and U2OS sarcoma cell lines.
Whole-exome sequence analysis of DNA from 2 siblings identified 83 gene variants that were shared between the siblings and considered as possible disease-causing changes. A heterozygous variant, R148S in enteric smooth muscle actin ?-2 (ACTG2), segregated with disease phenotype. Intestinal smooth muscle (muscularis propria) from individuals with FVM had reduced levels of cytoplasmic ACTG2 and abnormal accumulation of the protein into intracellular inclusions compared with controls. Sarcoma cells that expressed exogenous ACTG2(R148S) incorporated reduced amounts of this protein into actin filaments compared with cells expressing ACTG2(wt) (P
Notes
Comment In: Gastroenterology. 2012 Dec;143(6):1420-323085350
PubMed ID
22960657 View in PubMed
Less detail

Serum carbonic anhydrase III and myoglobin concentrations in acute myocardial infarction.

https://arctichealth.org/en/permalink/ahliterature50320
Source
Clin Chem. 1990 Apr;36(4):635-8
Publication Type
Article
Date
Apr-1990
Author
H K Väänänen
H. Syrjälä
P. Rahkila
J. Vuori
L M Melamies
V. Myllylä
T E Takala
Author Affiliation
Department of Anatomy, University of Oulu, Finland.
Source
Clin Chem. 1990 Apr;36(4):635-8
Date
Apr-1990
Language
English
Publication Type
Article
Keywords
Adult
Carbonic Anhydrases - blood
Comparative Study
Creatine Kinase - blood
Female
Humans
Male
Middle Aged
Muscle, Smooth - metabolism
Muscles - metabolism
Myocardial Infarction - blood - enzymology
Myoglobin - blood
Neuromuscular Diseases - enzymology
Radioimmunoassay
Research Support, Non-U.S. Gov't
Abstract
Serum concentrations of myoglobin (S-Myo) and carbonic anhydrase III (S-CA III; EC 4.2.1.1), a skeletal muscle-specific protein, were measured by RIA in 26 patients with acute myocardial infarction, 14 patients with neuromuscular diseases, and six healthy subjects before and after physical exercise. S-Myo was increased in infarct patients, whereas S-CA III was not altered. In patients with neuromuscular diseases and in healthy subjects after physical exercise, both S-Myo and S-CA III were significantly increased. S-CA III and S-Myo also showed identical peak times, 2 h postexercise. The S-Myo/S-CA III ratio was always higher in infarct patients than in the other groups. Thus, the combination of S-CA III and S-Myo determinations is useful to differentiate whether serum myoglobin is originating from myocardium or from skeletal muscle.
PubMed ID
2108824 View in PubMed
Less detail

11 records – page 1 of 2.