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Source
Appl Biochem Biotechnol. 2004;113-116:433-45
Publication Type
Article
Date
2004
Author
Carla C B Pereira
Mônica A P da Silva
Marta A P Langone
Author Affiliation
Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Lab I 221, Cidade Universitária, Brazil.
Source
Appl Biochem Biotechnol. 2004;113-116:433-45
Date
2004
Language
English
Publication Type
Article
Keywords
Biotechnology - methods
Chromatography, Gas
Enzymes - chemistry
Enzymes, Immobilized
Esters
Glycerides - biosynthesis - chemistry
Glycerol - chemistry
Laurates - chemistry
Lauric Acids - chemistry
Lipase - chemistry
Models, Theoretical
Monoglycerides
Research Support, Non-U.S. Gov't
Temperature
Time Factors
Abstract
The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55 degrees C, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.
PubMed ID
15054269 View in PubMed
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Large-scale metabolomic profiling identifies novel biomarkers for incident coronary heart disease.

https://arctichealth.org/en/permalink/ahliterature268418
Source
PLoS Genet. 2014 Dec;10(12):e1004801
Publication Type
Article
Date
Dec-2014
Author
Andrea Ganna
Samira Salihovic
Johan Sundström
Corey D Broeckling
Asa K Hedman
Patrik K E Magnusson
Nancy L Pedersen
Anders Larsson
Agneta Siegbahn
Mihkel Zilmer
Jessica Prenni
Johan Arnlöv
Lars Lind
Tove Fall
Erik Ingelsson
Source
PLoS Genet. 2014 Dec;10(12):e1004801
Date
Dec-2014
Language
English
Publication Type
Article
Keywords
Aged
Biomarkers - blood
C-Reactive Protein - metabolism
Coronary Disease - blood - epidemiology - genetics
Female
Follow-Up Studies
Gene Expression Profiling
Genetic Association Studies
Humans
Incidence
Linear Models
Longitudinal Studies
Lysophosphatidylcholines - blood
Male
Metabolomics
Middle Aged
Monoglycerides - blood
Polymorphism, Single Nucleotide
Prospective Studies
Reproducibility of Results
Risk assessment
Risk factors
Sphingomyelins - blood
Sweden
Abstract
Analyses of circulating metabolites in large prospective epidemiological studies could lead to improved prediction and better biological understanding of coronary heart disease (CHD). We performed a mass spectrometry-based non-targeted metabolomics study for association with incident CHD events in 1,028 individuals (131 events; 10 y. median follow-up) with validation in 1,670 individuals (282 events; 3.9 y. median follow-up). Four metabolites were replicated and independent of main cardiovascular risk factors [lysophosphatidylcholine 18:1 (hazard ratio [HR] per standard deviation [SD] increment?=?0.77, P-value
Notes
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PubMed ID
25502724 View in PubMed
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