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Activation of alloreactive natural killer cells is resistant to cyclosporine.

https://arctichealth.org/en/permalink/ahliterature57609
Source
Transplantation. 1997 Apr 27;63(8):1138-44
Publication Type
Article
Date
Apr-27-1997
Author
E. Petersson
Z. Qi
H. Ekberg
O. Ostraat
M. Dohlsten
G. Hedlund
Author Affiliation
Department of Tumor Immunology, The Wallenberg Laboratory, Lund University, Sweden.
Source
Transplantation. 1997 Apr 27;63(8):1138-44
Date
Apr-27-1997
Language
English
Publication Type
Article
Keywords
Animals
Antigens, CD3 - analysis
Cyclosporine - pharmacology
Drug Resistance - immunology
Female
Graft Survival - drug effects
Heart Transplantation - immunology
Interleukin-2 - pharmacology
Killer Cells - immunology
Lymphocyte Activation - drug effects
Male
Rats
Rats, Inbred BN
Rats, Inbred WF
Research Support, Non-U.S. Gov't
T-Lymphocytes, Cytotoxic - drug effects - immunology
Abstract
BACKGROUND: We have previously shown that cytotoxic T lymphocytes (CTL) with alloreactivity were induced when Wistar Furth (WF; RT1u) rats were immunized with allogeneic Brown Norway (BN; RT1n) cells. In contrast, when BN rats were immunized with WF cells, the allospecific response was confined to alloreactive natural killer (NK) cells, and no CTL activity was observed. In this study, the effect of cyclosporine (CsA) on the activation of alloreactive NK cells in vivo was analyzed. METHODS: Distinct peritoneal effector cells from rats immunized with allogenic cells with or without concomitant CsA and/or interleukin (IL) 2 treatment were tested for specific cytolytic activity. Furthermore, the presumptive role of NK cells in rejection immunity was addressed in a cardiac graft model. RESULTS: The results showed that doses of CsA that completely inhibited the activation of alloreactive CTL, only marginally affected the activation of alloreactive NK cells. We also showed that CsA treatment failed to prolong graft survival in BN recipients of WF hearts. Treatment of BN rats with CsA/IL-2 during immunization with allogeneic WF cells resulted in concomitant induction of alloreactive NK cells and alloreactive CTL. CONCLUSIONS: We have demonstrated that CsA failed to suppress the activation of alloreactive NK cells. Consequently, the cardiac graft survival in the donor-recipient combination known to activate alloreactive NK cells was not significantly prolonged by CsA treatment, emphasizing the involvement of NK cells as effectors in organ rejection. Furthermore, the parallel emergence of alloreactive NK cells and CTL only in the presence of CsA/IL-2 indicated that CsA interfered with alloreactive NK cell-associated suppression of CTL activated by allogeneic tissue.
PubMed ID
9133476 View in PubMed
Less detail

Activation of the immune system and systemic immune-complex deposits in Brown Norway rats with dental amalgam restorations.

https://arctichealth.org/en/permalink/ahliterature72544
Source
J Dent Res. 1998 Jun;77(6):1415-25
Publication Type
Article
Date
Jun-1998
Author
P. Hultman
U. Lindh
P. Hörsted-Bindslev
Author Affiliation
Department of Health and Environment, Linköping University, Sweden.
Source
J Dent Res. 1998 Jun;77(6):1415-25
Date
Jun-1998
Language
English
Publication Type
Article
Keywords
Analysis of Variance
Animals
Antibody Formation - drug effects
Antigen-Antibody Complex - analysis - blood
Autoimmune Diseases - chemically induced
Autoimmunity
Body Burden
Comparative Study
Copper - analysis
Dental Amalgam - toxicity
Dinitrobenzenes
Female
Immune Complex Diseases - chemically induced
Immunoglobulin E - blood
Laminin
Lymphocyte Activation - drug effects
Mercury - analysis - blood - pharmacokinetics
Rats
Rats, Inbred BN
Rats, Inbred Lew
Research Support, Non-U.S. Gov't
Silver - analysis
Spectrum Analysis, Mass
Statistics, nonparametric
Tissue Distribution
Abstract
Dental amalgam restorations are a significant source of mercury exposure in the human population, but their potential to cause systemic health effects is highly disputed. We examined effects on the immune system by giving genetically mercury-susceptible Brown Norway (BN) rats and mercury-resistant Lewis (LE) rats silver amalgam restorations in 4 molars of the upper jaw, causing a body burden similar to that described in human amalgam-bearers (from 250 to 375 mg amalgam/kg body weight). BN rats with amalgam restorations, compared with control rats given composite resinous restorations, developed a rapid activation of the immune system, with a maximum 12-fold increase of the plasma IgE concentration after 3 wks (p 0.05). After 12 wks, BN rats with amalgam restorations showed significantly increased (p spleen > cerebrum occipital lobe > cerebellum > liver > thymus, and the tissue silver concentration was significantly (p
PubMed ID
9649170 View in PubMed
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Adjuvant-induced arthritis in four inbred strains of rats. An in vitro study of peripheral T and B lymphocytes.

https://arctichealth.org/en/permalink/ahliterature14754
Source
Agents Actions. 1976 Feb;6(1-3):219-27
Publication Type
Article
Date
Feb-1976
Author
A. Kahan
F. Perlik
A. Le Go
F. Delbarre
J P Giroud
Source
Agents Actions. 1976 Feb;6(1-3):219-27
Date
Feb-1976
Language
English
Publication Type
Article
Keywords
Animals
Arthritis, Rheumatoid - immunology
B-Lymphocytes - immunology
Freund's Adjuvant
Lymphocyte Activation - drug effects
Mitogens - pharmacology
Rats
Rats, Inbred Strains
Skin Tests
Species Specificity
T-Lymphocytes - immunology
Abstract
The lymphoblastic response (LTT) to non-specific mitogens (PHA, PWM and ConA) of peripheral lymphocytes was investigated at days 0, 7, 14, 21 and 28 after adjuvant injection in four strains of inbred rats: Wistar (WAG), Long Evans (LE), Lewis (LEW) and Brown Norway (BN). LTT was assessed by using 18 hours H3 TdR incorporation in 5 days cultures of whole blood (micromethod). The statistical treatment of data, using principal components multifactorial analysis and analysis of variance showed a striking difference between strains. In control animals the responses to PHA and PWM were correlated and were higher in LE and WAG than in LEW and BN (BN=LEW less than LE=WAG). The response to ConA was independent of that to the other mitogens. It was generally low, but significantly higher in LEW and BN than in WAG and LE. In adjuvant-injected animals the responses to PHA and PWM were still correlated, but modified compared to control: in LE and LEW, but not in WAG and BN, a marked decrease of the response was found, reaching a minimum value within days 7 and 14. In the same time the response to ConA increased in the four strains, later in LE than in the others. However the intensity of the ConA response varied from one strain to another: it was constantly low in LE and WAG compared to LEW and BN. So the most striking modification of LTT were observed in LE and LEW, which both developed the most severe arthritis. However these different behaviours after adjuvant injection were not explained by the initial level of LTT to the different mitogens. These data suggest that the development of intense arthritis is associated with the proliferation and the release into the blood stream of a lymphocyte subpopulation, which exhibits a low response to PHA and PWM and a high response to ConA. These LTT modifications are not paralleled by quantitative variations of B-cells assessed by surface Ig immunofluorescent staining and EAC rosetting.
PubMed ID
1085095 View in PubMed
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The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.

https://arctichealth.org/en/permalink/ahliterature13769
Source
Clin Immunol. 2005 Feb;114(2):154-63
Publication Type
Article
Date
Feb-2005
Author
Andrew Johnston
Johann Eli Gudjonsson
Hekla Sigmundsdottir
Björn Runar Ludviksson
Helgi Valdimarsson
Author Affiliation
Department of Immunology, Landspitali University Hospital, 101 Reykjavik, Iceland.
Source
Clin Immunol. 2005 Feb;114(2):154-63
Date
Feb-2005
Language
English
Publication Type
Article
Keywords
Adenosine - pharmacology
Anti-Inflammatory Agents - pharmacology
Antigens, Bacterial - immunology
Apoptosis - drug effects - immunology
Cell Adhesion Molecules - antagonists & inhibitors - immunology
Flow Cytometry
Fucosyltransferases - biosynthesis - genetics
Humans
Integrins - immunology
Intercellular Adhesion Molecule-1 - genetics - immunology
Leucovorin - pharmacology
Lymphocyte Activation - drug effects - immunology
Membrane Glycoproteins - genetics - immunology
Methotrexate - pharmacology
RNA - chemistry - genetics
RNA, Messenger - biosynthesis - genetics
Receptors, Interleukin-2 - antagonists & inhibitors - immunology
Research Support, Non-U.S. Gov't
Reverse Transcriptase Polymerase Chain Reaction
Streptococcus pyogenes - immunology
T-Lymphocytes - cytology - drug effects - immunology
Abstract
Low-dose methotrexate (MTX) is an established and highly effective treatment for severe psoriasis and rheumatoid arthritis; however, its mechanism of action remains unclear. We investigated the effects of low-dose MTX on antigen-stimulated peripheral blood mononuclear cells and explored through which cellular pathways these effects are mediated. We show that MTX caused a dose-dependent suppression of T cell activation and adhesion molecule expression, and this was not due to lymphocyte apoptosis. The suppression of intercellular adhesion molecule (ICAM)-1 was adenosine and folate-dependent, while MTX suppression of the skin-homing cutaneous lymphocyte-associated antigen (CLA) was adenosine-independent. The effect of MTX on CLA, but not ICAM-1, required the constant presence of MTX in cultures. Thus, the suppression of T cell activation and T cell adhesion molecule expression, rather than apoptosis, mediated in part by adenosine or polyglutamated MTX or both, are important mechanisms in the anti-inflammatory action of MTX.
PubMed ID
15639649 View in PubMed
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Anti-proliferative effects of lichen-derived inhibitors of 5-lipoxygenase on malignant cell-lines and mitogen-stimulated lymphocytes.

https://arctichealth.org/en/permalink/ahliterature10919
Source
J Pharm Pharmacol. 1998 Jan;50(1):107-15
Publication Type
Article
Date
Jan-1998
Author
H M Ogmundsdóttir
G M Zoëga
S R Gissurarson
K. Ingólfsdóttir
Author Affiliation
Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavík.
Source
J Pharm Pharmacol. 1998 Jan;50(1):107-15
Date
Jan-1998
Language
English
Publication Type
Article
Keywords
4-Butyrolactone - analogs & derivatives - pharmacology
Comparative Study
Ethanol - pharmacology
Fibroblasts - drug effects
Humans
Lactones - pharmacology
Lichens
Lipoxygenase Inhibitors - pharmacology
Lymphocyte Activation - drug effects
Lymphocytes - drug effects
Research Support, Non-U.S. Gov't
Salicylic Acids - pharmacology
Tumor Cells, Cultured
Abstract
Several lichen species have been used traditionally as medicinal plants. It has previously been shown that two low-molecular-weight lichen metabolites, lobaric acid isolated from Stereocaulon alpinum Laur. and protolichesterinic acid isolated from Cetraria islandica L. (Ach.), have in-vitro inhibitory effects on arachidonate 5-lipoxygenase. We have studied the effects of these compounds on cultured cells from man, including three malignant cell-lines (T-47D and ZR-75-1 from breast carcinomas and K-562 from erythro-leukaemia), as well as normal skin fibroblasts and peripheral blood lymphocytes. Both test substances caused a significant reduction in DNA synthesis, as measured by thymidine uptake, in all three malignant cell-lines; the dose inducing 50% of maximum inhibition (ED50) was between 1.1 and 24.6 microg mL(-1) for protolichesterinic acid and between 14.5 and 44.7 microg mL(-1) for lobaric acid. The breast-cancer cell-lines were more sensitive than K-562. The proliferative response of mitogen-stimulated lymphocytes was inhibited with a mean ED50 of 8.4 microg mL(-1) and 24.5 microg mL(-1) for protolichesterinic acid and lobaric acid, respectively. These concentrations are of the same order of magnitude as the IC50 values in the 5-lipoxygenase assay. Significant cell death (assessed by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-( 4-sulfophenyl)-2H-tetrazolium) assay and trypan blue exclusion) occurred in the three malignant cell-lines at protolichesterinic acid and lobaric acid concentrations above 20 and 30 microg mL(-1), respectively. In K-562 morphological changes consistent with apoptosis were detected. Up to 38% cell death was observed at 20 microg mL(-1) for protolichesterinic acid and 15 microg mL(-1) for lobaric acid in mitogen-stimulated lymphocytes but unstimulated lymphocytes were clearly less sensitive. In contrast, the DNA synthesis, proliferation and survival of normal skin fibroblasts were not affected at doses up to 20 microg mL(-1) for protolichesterinic acid and 30 microg mL(-1) for lobaric acid. We conclude that the anti-proliferative and cytotoxic effects observed might be related to the 5-lipoxygenase inhibitory activity of protolichesterinic acid and lobaric acid. These results open up the opportunity for future studies of these lichen metabolites with regard to their anti-tumour and anti-inflammatory properties.
PubMed ID
9504441 View in PubMed
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Associations between gastrointestinal toxicity, micro RNA and cytokine production in patients undergoing myeloablative allogeneic stem cell transplantation.

https://arctichealth.org/en/permalink/ahliterature268773
Source
Int Immunopharmacol. 2015 Mar;25(1):180-8
Publication Type
Article
Date
Mar-2015
Author
Peter L Pontoppidan
Karina Jordan
Anting Liu Carlsen
Hilde Hylland Uhlving
Katrine Kielsen
Mette Christensen
Marianne Ifversen
Claus Henrik Nielsen
Per Sangild
Niels Henrik Helweg Heegaard
Carsten Heilmann
Henrik Sengeløv
Klaus Müller
Source
Int Immunopharmacol. 2015 Mar;25(1):180-8
Date
Mar-2015
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Biomarkers - metabolism
Cells, Cultured
Child
Child, Preschool
Citrulline - blood
Cytokines - blood
Denmark
Drug Therapy
Enterocytes - drug effects - immunology
Gene Expression Regulation - drug effects
Hematopoietic Stem Cell Transplantation
Humans
Infant
Inflammation Mediators - blood
Intestines - pathology
Leukocytes, Mononuclear - drug effects - immunology
Lymphocyte Activation - drug effects
Male
MicroRNAs - genetics - immunology - metabolism
Middle Aged
Myeloablative Agonists - therapeutic use
Prospective Studies
Transplantation Conditioning - methods
Young Adult
Abstract
Allogeneic hematopoietic stem cell transplantation (HSCT) is a procedure with a high risk of treatment related mortality. The primary aim of the present study was to examine associations between markers of gastrointestinal toxicity, markers of systemic inflammation, and plasma levels of microRNA (miRNA) -155 and -146a during the first month after HSCT. The secondary aim was to characterize the impact of the toxic-inflammatory response on the function of circulating leukocytes during immune recovery. Thirty HSCT patients were included. Gastrointestinal injury was monitored by toxicity scores, lactulose-mannitol test and plasma citrulline, as a measure of the enterocyte population. Nadir of citrulline and maximum of oral toxicity scores, intestinal permeability, CRP and plasma levels of IL-6 and IL-10 was seen at day +7 post-HSCT. miRNA-155 and mi-RNA-146a showed an inverse relation with significantly elevated miRNA-155 and decreased miRNA-146a levels, from day 0 to day +28 compared with pre-conditioning levels. Citrulline levels below the median at day +7 were associated with higher spontaneous production of IL-6 and TNF-a as well as higher in vitro stimulated production of IL-17A at day +21. This study is the first to demonstrate that toxic responses to chemotherapy are accompanied by differential regulation of miRNAs with opposing effects on immune regulation. We find that a proinflammatory miRNA profile is sustained during the first three weeks after the transplantation, indicating that these miRNAs may play a role in the regulation of the inflammatory environment during immune reconstitution after HSCT.
PubMed ID
25614225 View in PubMed
Less detail

[B-lymphocyte activity in healthy infants during the 1st year of life]

https://arctichealth.org/en/permalink/ahliterature60710
Source
Pediatr Akus Ginekol. 1978 Sep-Oct;(5):16-7
Publication Type
Article

Chlamydia pneumoniae-specific cell-mediated and humoral immunity in healthy people.

https://arctichealth.org/en/permalink/ahliterature52649
Source
Scand J Immunol. 1998 May;47(5):517-20
Publication Type
Article
Date
May-1998
Author
S. Halme
L. von Hertzen
A. Bloigu
J. Kaprio
M. Koskenvuo
M. Leinonen
P. Saikku
H M Surcel
Author Affiliation
National Public Health Institute, Department of Medical Microbiology, University of Oulu, Finland.
Source
Scand J Immunol. 1998 May;47(5):517-20
Date
May-1998
Language
English
Publication Type
Article
Keywords
Adult
Antibodies, Bacterial - blood - immunology
Antibody Formation - immunology
Antibody Specificity
Antigens, Bacterial - pharmacology
Chlamydia Infections - epidemiology
Chlamydophila pneumoniae - immunology
Comparative Study
Female
Finland - epidemiology
Humans
Immunity, Cellular - immunology
Immunoglobulin A - blood
Immunoglobulin G - blood
Leukocytes, Mononuclear - drug effects
Lymphocyte Activation - drug effects
Male
Middle Aged
Prevalence
Research Support, Non-U.S. Gov't
Sex Factors
Titrimetry
Abstract
In this work, cell-mediated immunity to Chlamydia pneumoniae was studied in 157 healthy individuals using lymphoproliferative assay and serum antibodies were analysed by microimmunofluorescence techniques. The C. pneumoniae-specific IgG antibodies were elevated more frequently and the geometric mean titres for IgG (67.5 versus 44.1; P = 0.05) and IgA (14.9 versus 11.3; P = 0.025) antibodies were significantly higher in males than in females. However, no gender-dependent differences were observed in cellular reactivity to C. pneumoniae, since the median cellular responses were similar (stimulation indices 7.5) in men and women. Although the cell-mediated and humoral responses to C. pneumoniae did not correlate clearly, elevated IgG antibodies were associated with slightly higher lymphocyte proliferation in comparison to all subjects (15.5 versus 7.5) and significantly stronger in comparison to those with persistently elevated IgA (> 80) antibodies (15.5 versus 3.5; P = 0.023). Further studies are needed to evaluate a possible role of reduced cellular reactivity in the cause of chronic C. pneumoniae infection.
PubMed ID
9627138 View in PubMed
Less detail

[Clinical importance of lymphocyte blast transformation to specific and nonspecific mitogens in kidney pathology in children]

https://arctichealth.org/en/permalink/ahliterature41762
Source
Pediatr Akus Ginekol. 1978;(3):15-6
Publication Type
Article
Date
1978

36 records – page 1 of 4.