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Human isolates of Listeria monocytogenes in Sweden during half a century (1958-2010).

https://arctichealth.org/en/permalink/ahliterature259026
Source
Epidemiol Infect. 2014 Nov;142(11):2251-60
Publication Type
Article
Date
Nov-2014
Author
G. Lopez-Valladares
W. Tham
V Singh Parihar
S. Helmersson
B. Andersson
S. Ivarsson
C. Johansson
H. Ringberg
I. Tjernberg
B. Henriques-Normark
M-L Danielsson-Tham
Source
Epidemiol Infect. 2014 Nov;142(11):2251-60
Date
Nov-2014
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Age Distribution
Aged
Aged, 80 and over
Animals
Child
Child, Preschool
Databases, Factual
Electrophoresis, Gel, Pulsed-Field - methods
Female
Food Contamination - prevention & control - statistics & numerical data
Humans
Infant
Listeria monocytogenes - classification - pathogenicity
Listeriosis - diagnosis - epidemiology
Male
Middle Aged
Pregnancy
Prevalence
Retrospective Studies
Risk assessment
Salmon
Seafood - adverse effects - analysis
Serotyping - methods
Sex Distribution
Sweden - epidemiology
Time Factors
Young Adult
Abstract
Isolates of Listeria monocytogenes (n = 932) isolated in Sweden during 1958-2010 from human patients with invasive listeriosis were characterized by serotyping and pulsed-field gel electrophoresis (PFGE) (AscI). Of the 932 isolates, 183 different PFGE types were identified, of which 83 were each represented by only one isolate. In all, 483 serovar 1/2a isolates were distributed over 114 PFGE types; 90 serovar 1/2b isolates gave 32 PFGE types; 21 serovar 1/2c isolates gave nine PFGE types; three serovar 3b isolates gave one PFGE type; and, 335 serovar 4b isolates gave 31 PFGE types. During the 1980s in Sweden, several serovar 4b cases were associated with the consumption of European raw soft cheese. However, as cheese-production hygiene has improved, the number of 4b cases has decreased. Since 1996, serovar 1/2a has been the dominant L. monocytogenes serovar in human listeriosis in Sweden. Therefore, based on current serovars and PFGE types, an association between human cases of listeriosis and the consumption of vacuum-packed gravad and cold-smoked salmon is suggested.
PubMed ID
24480252 View in PubMed
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Subtyping of Listeria monocytogenes isolates recovered from retail ready-to-eat foods, processing plants and listeriosis patients in Sweden 2010.

https://arctichealth.org/en/permalink/ahliterature108260
Source
Int J Food Microbiol. 2013 Aug 16;166(1):186-92
Publication Type
Article
Date
Aug-16-2013
Author
S Thisted Lambertz
S. Ivarsson
G. Lopez-Valladares
M. Sidstedt
R. Lindqvist
Author Affiliation
Science Department, National Food Agency, Sweden. sula@slv.se
Source
Int J Food Microbiol. 2013 Aug 16;166(1):186-92
Date
Aug-16-2013
Language
English
Publication Type
Article
Keywords
Aged
Animals
Electrophoresis, Gel, Pulsed-Field
Environmental Microbiology
Female
Food Handling
Food Microbiology
Genotype
Humans
Listeria monocytogenes - classification - genetics - isolation & purification
Listeriosis - epidemiology - microbiology
Male
Polymerase Chain Reaction
Serotyping
Sweden - epidemiology
Abstract
Identification and prioritisation of food safety interventions requires an understanding of the relationship between food, pathogens and cases. Such understanding can be gained through different approaches, e.g. microbial subtyping to attribute cases of foodborne disease to food vehicles or other sources of illness. In this study, Listeria monocytogenes isolates (n=166) from (i) three categories of ready-to-eat (RTE) foods, (ii) food processing plant environments, and (iii) human listeriosis cases, all sampled during 2010 in Sweden, were subtyped. In addition, 121 isolates from human listeriosis cases, collected 2005-2009, were subtyped. Subtyping consisted of both serotyping (conventional method and PCR) and genotyping using pulsed-field gel electrophoresis (PFGE). Serotype 1/2a dominated in all three groups of isolates (range 73-96%). Eighteen percent of the human isolates (2010) belonged to serotype 4b, but only 1.4% of the food isolates. The food isolates differentiated into 19 pulsotypes (ID=0.843), the human isolates collected 2010 into 31 pulsotypes (ID=0.950) and the processing plant isolates into 22 pulsotypes (ID=0.991). Six of the pulsotypes were shared between the food and human isolates. These pulsotypes comprised 42% of the human isolates and 59% of the food isolates. For some processing plants, there was suggested persistence of one or more specific L. monocytogenes strains, as indicated by repetitive isolation of the same pulsotype from food. This study indicated the presence of L. monocytogenes in the processing plant environment as a likely source of contamination of gravad and cold-smoked fish, and this food category as an important source of human exposure to the pathogen.
PubMed ID
23911759 View in PubMed
Less detail