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[Differentiantion of Listeria monocytogenes strains isolated in the Far East and European part of Russia on the basis of polymorphism of genes encoding invasion factors].

https://arctichealth.org/en/permalink/ahliterature152832
Source
Zh Mikrobiol Epidemiol Immunobiol. 2008 Nov-Dec;(6):10-4
Publication Type
Article
Author
E A Zaitseva
K R Beliaev
I Iu Egorova
A I Suniaikin
N M Pukhovskaia
Iu S Musatov
L I Ivanov
D V Kolbasov
G P Somov
A L Gintsburg
S A Ermolaeva
Source
Zh Mikrobiol Epidemiol Immunobiol. 2008 Nov-Dec;(6):10-4
Language
Russian
Publication Type
Article
Keywords
Bacterial Proteins - genetics
Bacterial Typing Techniques
Genes, Bacterial - genetics
Humans
Listeria monocytogenes - classification - genetics - pathogenicity
Listeriosis - epidemiology
Membrane Proteins - genetics
Molecular Epidemiology
Polymorphism, Genetic
Ribose-Phosphate Pyrophosphokinase - genetics
Russia - epidemiology
Siberia - epidemiology
Virulence - genetics
Virulence Factors - genetics
Abstract
Forty Listeria monocytogenes isolates obtained in European and Far East regions of Russia were differentiated on the basis of polymorphism of 5 markergenes. Total length of concatemers obtained after sequencing of internal fragments of genes inlA, inlB, inlC, inlE and prs was 3029 b.p. Comparative analysis of concatemers' sequences revealed 237 variable nucleotides. Totally, 25 sequence types were revealed, and isolates from European and Far East regions belonged to different types. On the dendrogram isolates split on 2 clusters, which correspond to early described phylogenetic lines of L. monocytogenes specie. Isolates obtained in European and Far East regions formed independent subclusters within main clusters. Fifteen clinical isolates of L. monocytogenes belonged to 7 different types. Analysis of epidemiologic data on time and place of isolates obtaining suggested that isolates of the same sequence type are epidemiologically related and might represent one strain; index of discrimination for proposed typing method was calculated as 0.982.
PubMed ID
19186537 View in PubMed
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[Regularities of the ubiquitous polyhostal microorganisms selection by the example of three taxa].

https://arctichealth.org/en/permalink/ahliterature265544
Source
Mol Biol (Mosk). 2015 May-Jun;49(3):430-41
Publication Type
Article
Author
O L Voronina
M S Kunda
N N Ryzhova
E I Aksenova
A N Semenov
M A Kurnaeva
Yu V Ananyina
V G Lunin
A L Gintsburg
Source
Mol Biol (Mosk). 2015 May-Jun;49(3):430-41
Language
Russian
Publication Type
Article
Keywords
Animals
Burkholderia - classification - genetics - isolation & purification
Burkholderia Infections - epidemiology - microbiology - transmission
DNA, Bacterial - genetics
Genome, Bacterial
Genotype
Humans
Leptospira - classification - genetics - isolation & purification
Leptospirosis - epidemiology - microbiology - transmission
Listeria monocytogenes - classification - genetics - isolation & purification
Listeriosis - epidemiology - microbiology - transmission
Multilocus Sequence Typing
Phylogeny
Rodentia - microbiology
Russia - epidemiology
Abstract
The investigation of the bacterial populations' heterogeneity contributes to the control of natural foci, causative agents of nosocomial infections, to the analysis of the microbial evolution. Multilocus sequence typing (MLST) was employed for the analysis of the diversity and features of the distribution of polyhostal ubiquitous microorganisms of the genera Burkholderia, Leptospira, and Listeria, which belong to three bacterial phyla: Proteobacteria, Spirochaetes, and Firmicutes. According to the bacterial samples analysis microbial genotypes prevalent and unique to Russia were identified; their occurrence in different Federal Regions was investigated; their similarity with global spread genotypes was appreciated. Obtained results allowed identifying common regularities of the selection of the microorganisms capable to cause the diseases of human and animals. The formation of genotypes that are most pathogenic for the host was demonstrated for all groups of bacteria. Leptospira spp. and Listeria monocytogenes strains with these genotypes have been circulating for a long time, being supported by natural foci. The formation of a wide variety of genotypes with different pathogenicity was demonstrated in the local geographic areas. In Russia, the zonal difference in all three groups of bacteria is most clearly traced to the Far Eastern Federal Region. The results are thought to contribute to analyzing the factors of selection and the phylogeny of the taxa under study.
PubMed ID
26107896 View in PubMed
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