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Design and characterization of a direct ELISA for the detection and quantification of leucomalachite green.

https://arctichealth.org/en/permalink/ahliterature134093
Source
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Jun;28(6):731-9
Publication Type
Article
Date
Jun-2011
Author
Gurmit Singh
Terence Koerner
Jean-Marc Gelinas
Michael Abbott
Beth Brady
Anne-Catherine Huet
Caroline Charlier
Philippe Delahaut
Samuel Benrejeb Godefroy
Author Affiliation
Food Directorate, Health Canada, Ottawa, Ontario, Canada.
Source
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Jun;28(6):731-9
Date
Jun-2011
Language
English
Publication Type
Article
Keywords
Animals
Anti-Infective Agents - analysis
Aquaculture - legislation & jurisprudence
Canada
Coloring Agents - analysis
Cross Reactions
Drug Residues - analysis - standards
Enzyme-Linked Immunosorbent Assay
Food Contamination
Food Inspection - methods
Humans
Legislation, Food
Limit of Detection
Reproducibility of Results
Rosaniline Dyes - analysis - chemistry
Seafood - analysis - standards
Solid Phase Extraction
Tilapia
Veterinary Drugs - analysis
Water Pollutants, Chemical - analysis
Abstract
Malachite green (MG), a member of the N-methylated triphenylmethane class of dyes, has long been used to control fungal and protozoan infections in fish. MG is easily absorbed by fish during waterborne exposure and is rapidly metabolized into leucomalachite green (LMG), which is known for its long residence time in edible fish tissue. This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of LMG in fish tissue. This development includes a simple and versatile method for the conversion of LMG to monodesmethyl-LMG, which is then conjugated to bovine serum albumin (BSA) to produce an immunogenic material. Rabbit polyclonal antibodies are generated against this immunogen, purified and used to develop a direct competitive enzyme-linked immunosorbent assay (ELISA) for the screening and quantification of LMG in fish tissue. The assay performed well, with a limit of detection (LOD) and limit of quantification (LOQ) of 0.1 and 0.3 ng g(-1) of fish tissue, respectively. The average extraction efficiency from a matrix of tilapia fillets was approximately 73% and the day-to-day reproducibility for these extractions in the assay was between 5 and 10%.
Notes
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PubMed ID
21623496 View in PubMed
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