Skip header and navigation

1 records – page 1 of 1.

Source
Appl Biochem Biotechnol. 2004;113-116:433-45
Publication Type
Article
Date
2004
Author
Carla C B Pereira
Mônica A P da Silva
Marta A P Langone
Author Affiliation
Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Lab I 221, Cidade Universitária, Brazil.
Source
Appl Biochem Biotechnol. 2004;113-116:433-45
Date
2004
Language
English
Publication Type
Article
Keywords
Biotechnology - methods
Chromatography, Gas
Enzymes - chemistry
Enzymes, Immobilized
Esters
Glycerides - biosynthesis - chemistry
Glycerol - chemistry
Laurates - chemistry
Lauric Acids - chemistry
Lipase - chemistry
Models, Theoretical
Monoglycerides
Research Support, Non-U.S. Gov't
Temperature
Time Factors
Abstract
The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55 degrees C, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.
PubMed ID
15054269 View in PubMed
Less detail