Skip header and navigation

Refine By

   MORE

8 records – page 1 of 1.

Clinical and immunologic responses to multiple doses of IMVAMUNE (Modified Vaccinia Ankara) followed by Dryvax challenge.

https://arctichealth.org/en/permalink/ahliterature87379
Source
Vaccine. 2007 Dec 12;25(51):8562-73
Publication Type
Article
Date
Dec-12-2007
Author
Frey Sharon E
Newman Frances K
Kennedy Jeffrey S
Sobek Vera
Ennis Francis A
Hill Heather
Yan Lihan K
Chaplin Paul
Vollmar Jens
Chaitman Bernard R
Belshe Robert B
Author Affiliation
Department of Medicine, Saint Louis University School of Medicine and National Institute of Allergy and Infectious Diseases Vaccine Treatment and Evaluation Unit, 1100 S. Grand Blvd (DRC-8), St. Louis, MO 63104, USA. freyse@slu.edu
Source
Vaccine. 2007 Dec 12;25(51):8562-73
Date
Dec-12-2007
Language
English
Publication Type
Article
Keywords
Adolescent
Adult
Chemistry, Pharmaceutical
Enzyme-Linked Immunosorbent Assay
Erythema - immunology
Female
Heart Diseases - genetics
Humans
Immunoglobulin G - biosynthesis - immunology
Interferon Type II - biosynthesis - genetics
Male
Plaque Assay
Skin - pathology
Smallpox Vaccine - adverse effects - immunology
T-Lymphocytes - immunology
Vaccines, Attenuated - adverse effects - immunology
Vaccinia virus - immunology
Abstract
Smallpox vaccination with replication deficient vaccinia strains such as Modified Vaccinia Ankara (MVA) may induce protective immunity with improved safety and tolerability profiles compared with currently available smallpox vaccines. Ninety subjects were randomized equally to six groups in a partially blinded, randomized, controlled clinical trial. IMVAMUNE (MVA-BN, Bavarian Nordic A/S, Kvistgård, Denmark) vaccine or placebo was administered at Study Days 0 and 28 by subcutaneous or intramuscular injection and five groups were challenged with Dryvax at study Day 112. Vaccination with two doses of IMVAMUNE was safe and well tolerated compared to Dryvax. IMVAMUNE produced comparable cellular and humoral immune responses to one dose of Dryvax and the immunity induced appears robust 90 days post-vaccination by evidence of attenuated primary cutaneous reaction responses following Dryvax. IMVAMUNE vaccination prior to Dryvax reduced virus replication at the Dryvax site, decreased the size of the primary cutaneous lesion, and decreased the time to healing but did not completely ameliorate the immune response.
PubMed ID
18036708 View in PubMed
Less detail

Conjugates of ovalbumin and monomethoxypolyethylene glycol abolish late allergic responses and decrease IL-4 and IL-5 mRNA expression in the rat.

https://arctichealth.org/en/permalink/ahliterature9604
Source
Pulm Pharmacol Ther. 2003;16(6):361-9
Publication Type
Article
Date
2003
Author
J-P Lavoie
K. Maghni
R. Taha
X-X Yang
G M Lang
A H Sehon
Q A Hamid
J G Martin
Author Affiliation
Department de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Sicotte CP5000, St-Hyacinthe, Que., Canada J2S 7C6. jean-pierre.lavoie@umontreal.ca
Source
Pulm Pharmacol Ther. 2003;16(6):361-9
Date
2003
Language
English
Publication Type
Article
Keywords
Animals
Asthma - drug therapy - metabolism - physiopathology
Bronchoalveolar Lavage Fluid - chemistry - cytology
Bronchoconstriction - drug effects
Histamine - analysis
Interferon Type II - biosynthesis - genetics
Interleukin-4 - biosynthesis - genetics
Interleukin-5 - biosynthesis - genetics
Ovalbumin - administration & dosage - immunology - therapeutic use
Polyethylene Glycols - administration & dosage - therapeutic use
RNA, Messenger - biosynthesis
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Abstract
The purpose of this study was to test the therapeutic potential of monomethoxypolyethylene glycol (mPEG) conjugated-allergen using a rodent model of allergic asthma. Previously, this conjugate has been shown to possess the dual capacity of inducing long-term ovalbumin (OA)-specific suppression of the antibody response and inactivating rat mast cells that have been sensitized with murine IgE to OA. Ovalbumin sensitized and challenged Brown Norway rats were studied. Fourteen days after sensitization, a test group of six rats received mPEG-OA solution intratracheally and were challenged 30 min later with aerosolized OA. Another group of seven sensitized rats was similarly challenged with OA 30 min after intratracheal administration of normal saline. A group of six sensitized rats received mPEG-OA solution intratracheally but were challenged with normal saline. Another group of seven sensitized rats received mPEG-BSA solution intratracheally and were challenged 30 min later with aerosolized OA. A final group of five unsensitized rats were neither challenged nor medicated intratracheally. Pulmonary resistance was measured before and for 8 h following inhalation challenge. mPEG-OA treatment had an inhibitory effect on the allergic late airway response, but the early response was not significantly altered. Both mPEG-OA and mPEG-BSA reduced the total cells, eosinophils and neutrophils, in bronchoalveolar lavage and decreased the expression of IL-4, IL-5 and IFN-gamma mRNA. In conclusion, mPEG-OA can prevent the development of allergen-induced late airway responses and reduce airway Th2-type cytokine expression whereas mPEG conjugated to an irrelevant antigen (BSA) is anti-inflammatory but does not affect the late response.
PubMed ID
14580928 View in PubMed
Less detail

The effects of CD8+gammadelta T cells on late allergic airway responses and airway inflammation in rats.

https://arctichealth.org/en/permalink/ahliterature57418
Source
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Publication Type
Article
Date
Sep-2003
Author
Susumu Isogai
Alexandra Rubin
Karim Maghni
David Ramos-Barbon
Rame Taha
Yasuyuki Yoshizawa
Qutayba Hamid
James G Martin
Author Affiliation
Meakins Christie Laboratories, Department of Medicine, McGill University, 3623 St Urbain, Montreal, Quebec, Canada H2X 2P2.
Source
J Allergy Clin Immunol. 2003 Sep;112(3):547-55
Date
Sep-2003
Language
English
Publication Type
Article
Keywords
Adoptive Transfer
Allergens - administration & dosage
Animals
CD8-Positive T-Lymphocytes - immunology
Cytokines - biosynthesis - genetics
Eosinophilia - immunology
Immunoglobulin E - blood
Interferon Type II - biosynthesis - genetics
Interleukin-4 - biosynthesis - genetics
Male
Models, Immunological
Ovalbumin - administration & dosage - immunology
RNA, Messenger - genetics - metabolism
Rats
Rats, Inbred BN
Receptors, Antigen, T-Cell, gamma-delta - metabolism
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity - etiology - immunology
T-Lymphocyte Subsets - immunology
Abstract
BACKGROUND: Gamma-delta (gammadelta) T cells regulate immune responses to foreign protein at mucosal surfaces. Whether they can modify allergen-induced early (EAR) and late airway responses (LAR) is unknown. OBJECTIVE: We have tested the hypothesis that the CD8+ subtype of gammadelta T cells decreases allergen-induced LAR and airway eosinophilia in the rat. METHODS: Brown Norway rats were administered, intraperitoneally, 3.5 x 10(4) lymph node CD8+gammadelta T cells from naive or sensitized rats. The recipients were sensitized to ovalbumin (OVA) in Al(OH)(3) 3 days after cell transfer and challenged with aerosolized OVA 14 days later. Serum IgE was measured before allergen challenge. After challenge, lung resistance was monitored for 8 hours and then bronchoalveolar lavage (BAL) was analyzed for eosinophil major basic protein (MBP), IL-4, IL-5, IL-13, and IFN-gamma messenger RNA-expressing cells. RESULTS: gammadelta T cells from naive donors significantly decreased LAR in OVA-challenged sensitized rats, whereas MBP(+) eosinophils were decreased by both gammadelta T cells from naive and sensitized donors. EAR and serum IgE levels were unchanged. The expression of IL-4, IL-5, and IL-13 by BAL cells of gammadelta T cell recipients was attenuated compared with OVA-challenged controls. This was accompanied by an increase in the expression of IFN-gamma. CONCLUSIONS: Our results are consistent with a suppressive role of CD8+gammadelta T cells on allergic airway responses. However, only gammadelta T cells from naive donors inhibit LAR.
PubMed ID
13679814 View in PubMed
Less detail

Effects of IL-4 and IL-12 on experimental immune-mediated blepharoconjunctivitis in Brown Norway rats.

https://arctichealth.org/en/permalink/ahliterature50855
Source
Clin Exp Immunol. 2000 Oct;122(1):28-34
Publication Type
Article
Date
Oct-2000
Author
A. Ozaki
A. Fukushima
K. Fukata
H. Ueno
Author Affiliation
Laboratory of Immunology, Department of Ophthalmology, Kochi Medical School, Nankoku, Japan.
Source
Clin Exp Immunol. 2000 Oct;122(1):28-34
Date
Oct-2000
Language
English
Publication Type
Article
Keywords
Allergens - immunology
Animals
Blepharitis - drug therapy - immunology
Cells, Cultured
Conjunctivitis - drug therapy - immunology
Disease Models, Animal
Interferon Type II - biosynthesis - genetics
Interleukin-10 - biosynthesis - genetics
Interleukin-12 - genetics - immunology - therapeutic use
Interleukin-4 - genetics - immunology - therapeutic use
Lymph Nodes - cytology
Male
Pollen - immunology
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Abstract
IL-12 and IL-4 are critical cytokines for Th1 and Th2 differentiation, respectively. To assess the roles of these cytokines in the development of experimental immune-mediated blepharoconjunctivitis (EC) in Brown Norway (BN) rats, their effects were tested either in vitro or in vivo. Draining lymph node cells from rats immunized with ragweed pollen (RW) in Al(OH)3 were collected and cultured for 3 days with RW in the presence of IL-4, IL-12, or PBS as a control. After harvesting the culture supernatants for cytokine ELISA and the cells for cytokine reverse transcriptase-polymerase chain reaction, 10 million cells were injected intravenously into syngeneic recipient rats (n = 12 per group). The rats were challenged with RW by eye drops 4 days after transfer. Eyes were harvested for histology 24 h later. Furthermore, IL-12 (500 ng per injection) or PBS was injected intraperitoneally every other day seven times from the day of active immunization (n = 6 per group). One day after the last injection, rats were challenged and EC was evaluated as above. Transfer of cells with IL-4 in vitro augmented eosinophilic infiltration in the conjunctiva compared with the other two groups, whereas IL-12 in vitro suppressed eosinophilic infiltration and increased lymphocytic infiltration. Interferon-gamma production was augmented by IL-12. IL-4 RNA expression was augmented by IL-4. IL-12 administration in vivo augmented lymphocytic infiltration in the conjunctiva without affecting infiltration of eosinophils. In conclusion, IL-4 and IL-12 either in vitro or in vivo augmented Th2 and Th1 immunity, respectively, thus leading to distinct histological features of EC.
PubMed ID
11012614 View in PubMed
Less detail

Gold is a T cell polyclonal activator in BN and LEW rats but favors IL-4 expression only in autoimmune prone BN rats.

https://arctichealth.org/en/permalink/ahliterature14023
Source
Eur J Immunol. 2001 Aug;31(8):2266-76
Publication Type
Article
Date
Aug-2001
Author
M. Savignac
A. Badou
C. Delmas
J F Subra
S. De Cramer
P. Paulet
G. Cassar
P. Druet
A. Saoudi
L. Pelletier
Author Affiliation
INSERM U28, IFR 30 CHU Purpan, Toulouse, France.
Source
Eur J Immunol. 2001 Aug;31(8):2266-76
Date
Aug-2001
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Monoclonal - immunology - pharmacology
Autoimmunity - drug effects - immunology
B-Lymphocytes - drug effects - immunology
CD8-Positive T-Lymphocytes - drug effects - immunology - metabolism
Calcium - metabolism
Calcium Signaling - drug effects
Chlorides - pharmacology
Gene Expression Regulation - drug effects
Gold - pharmacology
Gold Compounds - pharmacology
Immunoglobulin E - immunology
Immunoglobulin G - immunology
Interferon Type II - biosynthesis - genetics - immunology
Interleukin-4 - biosynthesis - genetics - immunology
Lymphocyte Activation - drug effects
Phosphorylation - drug effects
RNA, Messenger - genetics - metabolism
Rats
Rats, Inbred BN
Rats, Inbred Lew
Research Support, Non-U.S. Gov't
Spleen - cytology - drug effects - immunology
T-Lymphocytes - drug effects - immunology - metabolism
Th2 Cells - drug effects - immunology - metabolism
Abstract
Gold salts are beneficial in the treatment of rheumatoid arthritis but may induce immune-mediated disorders in predisposed patients. Gold salts induce Th2-dependent autoimmunity in Brown-Norway (BN) rats but not in Lewis (LEW) rats. The aim of this study was to define molecular targets of gold salts and to approach why LEW rats are resistant. Gold salts act on early steps of transduction in T cells from BN and LEW rats since they trigger tyrosine phosphorylation of numerous proteins including p56(lck) and a calcium signal which results in IL-4 and IFN-gamma expression by BN and LEW T cells. However, the IL-4 response was favored in BN spleen cells in vitro and in vivo. IFN-gamma, produced in part by CD8(+) cells, contributes to the resistance of LEW rats since gold salt-injected LEW rats receiving anti-CD8 or anti-IFN-gamma mAb displayed the parameters characteristics of gold salt-induced Th2 autoimmunity although to a lesser extent than in BN rats. Gold salts transduce a signal in BN and LEW spleen cells resulting in IL-4 and IFN-gamma gene transcription with a preferential IL-4 response in BN rats, a Th2-prone strain, while IFN-gamma contributes to the resistance of LEW rats.
PubMed ID
11477538 View in PubMed
Less detail

IL-4, IL-5 and IFN-gamma mRNA expression in pulmonary lymphocytes in equine heaves.

https://arctichealth.org/en/permalink/ahliterature15211
Source
Vet Immunol Immunopathol. 2004 Jan;97(1-2):87-96
Publication Type
Article
Date
Jan-2004
Author
Marie-Eve Cordeau
Philippe Joubert
Oday Dewachi
Qutayba Hamid
Jean-Pierre Lavoie
Author Affiliation
Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Sicotte, St-Hyacinthe, Que., Canada J2S 7C6.
Source
Vet Immunol Immunopathol. 2004 Jan;97(1-2):87-96
Date
Jan-2004
Language
English
Publication Type
Article
Keywords
Animals
Bronchoalveolar Lavage Fluid - cytology - immunology
Female
Horse Diseases - genetics - immunology - metabolism
Horses
In Situ Hybridization - veterinary
Interferon Type II - biosynthesis - genetics - immunology
Interleukin-4 - biosynthesis - genetics - immunology
Interleukin-5 - biosynthesis - genetics - immunology
Lung Diseases, Obstructive - genetics - immunology - metabolism - veterinary
Male
RNA, Messenger - biosynthesis - blood - genetics
Research Support, Non-U.S. Gov't
Respiratory Function Tests - veterinary
Respiratory Hypersensitivity - genetics - immunology - metabolism - veterinary
Statistics, nonparametric
Abstract
Heaves is a common condition of horses of cold climate that is characterized by small airway inflammation and obstruction following exposure of susceptible horses to moldy hay and straw. It has been shown that helper T lymphocytes (Th) orchestrate the inflammatory response in asthma and in various animal models of allergic lung diseases by the release of Th2-type cytokines. Results of previous studies indicate that a predominant expression of Th2-type response by airway cells may also be present in heaves. To evaluate the temporal mRNA expression of Th1 (IFN-gamma) and Th2 (IL-4, IL-5) type cytokines in heaves and their relationship to clinical disease, we studied the pulmonary mechanics and cytokine mRNA expression (IL-4, IL-5 and IFN-gamma) in bronchoalveolar lavage lymphocytes of horses with heaves (n=6) and control (n=6) before and after 24h and 9 days of continuous natural inhalation challenge. Starting 24h after challenge horses with heaves, but not control horses, had a significant increase in pulmonary elastance and the number of lymphocytes expressing mRNA for IL-4 and IL-5. These changes were further increased at 9 days, at which time the number of cells positive for IFN-gamma mRNA was decreased. In this study we have shown that BAL lymphocytes of horses with heaves during clinical exacerbation have a predominant Th2-type cytokine response and that this response coincides in time with the presence of airway obstruction.
PubMed ID
14700540 View in PubMed
Less detail

Inhibition of inducible nitric oxide synthase intensifies injury and functional deterioration in autoimmune interstitial nephritis.

https://arctichealth.org/en/permalink/ahliterature57581
Source
J Immunol. 1997 Dec 15;159(12):6266-75
Publication Type
Article
Date
Dec-15-1997
Author
F B Gabbai
C. Boggiano
T. Peter
S. Khang
C. Archer
D P Gold
C J Kelly
Author Affiliation
Department of Medicine, University of California, San Diego 92161, USA.
Source
J Immunol. 1997 Dec 15;159(12):6266-75
Date
Dec-15-1997
Language
English
Publication Type
Article
Keywords
Administration, Oral
Animals
Autoimmune Diseases - enzymology - pathology - physiopathology
Basement Membrane - immunology
Enzyme Induction - immunology
Freund's Adjuvant - immunology
Heymann Nephritis Antigenic Complex
Immunoglobulin G - biosynthesis
Interferon Type II - biosynthesis - genetics
Kidney Cortex - enzymology
Kidney Tubules - enzymology - immunology
Lysine - administration & dosage - analogs & derivatives
Male
Membrane Glycoproteins - immunology
NG-Nitroarginine Methyl Ester - administration & dosage
Nephritis, Interstitial - enzymology - immunology - pathology - physiopathology
Nitric Oxide Synthase - biosynthesis
Nitric Oxide Synthase Type II
Nitrites - metabolism
RNA, Messenger - biosynthesis - genetics
Rats
Rats, Inbred BN
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Abstract
T lymphocytes are exquisitely sensitive to the antiproliferative effects of nitric oxide. We examined the effects of oral administration of two nitric oxide synthase inhibitors, Nw-nitro-L-arginine methyl ester (L-NAME) and L-N6-(1-iminoethyl)lysine (L-NIL), on the course of T cell-dependent autoimmune interstitial nephritis in Brown Norway rats. Kidneys from rats immunized to produce interstitial nephritis display a net generation of nitric oxide end products. By immunohistochemical staining, the cytokine-inducible nitric oxide synthase (iNOS) is expressed in cortical tubular epithelial cells. Treatment with either inhibitor results in markedly more severe disease following immunization. Animals receiving L-NAME were hypertensive, while those treated with L-NIL, a highly selective inhibitor of iNOS, were not. Evaluation of the expression of IFN-gamma, IL-2, and IL-4 in diseased kidneys by quantitative reverse transcriptase-PCR demonstrated that L-NAME-treated animals displayed significantly augmented levels of IFN-gamma and IL-2 with preserved ratios of IFN-gamma/IL-4 and IL-2/IL-4, while L-NIL-treated animals had augmented levels of IL-2 and IFN-gamma with augmented IFN-gamma/IL-4 and IL-2/IL-4 ratios. Animals treated with L-NAME or L-NIL both had augmented Ag-specific IgG responses. The L-NAME group demonstrated increases in both the IgG2a and IgG1 subtypes, with a constant IgG2a/IgG1 ratio, while the L-NIL group demonstrated an increase in the ratio of the IgG2a/IgG1 response. These Ab and cytokine data suggest that the L-NIL-treated animals had a skewing of their immune response toward a Th1-like response. We conclude that in autoimmune interstitial nephritis, generation of nitric oxide through the iNOS pathway has host-protective effects, and suggest that this may be broadly applicable to T cell-mediated pathologies.
PubMed ID
9550431 View in PubMed
Less detail

Local cytokine messenger ribonucleic acid expression and in vitro allergic late phase responses in Brown-Norway rats.

https://arctichealth.org/en/permalink/ahliterature15744
Source
Eur Respir J. 1998 Mar;11(3):630-5
Publication Type
Article
Date
Mar-1998
Author
E M Minshall
R J Dandurand
Q. Hamid
D H Eidelman
Author Affiliation
Meakins-Christie Laboratories, Montreal Chest Institute Research Center, Royal Victoria and Montreal General Hospitals, McGill University, Quebec, Canada.
Source
Eur Respir J. 1998 Mar;11(3):630-5
Date
Mar-1998
Language
English
Publication Type
Article
Keywords
Animals
Antigens - immunology
Asthma - immunology - metabolism - physiopathology
In Situ Hybridization
Interferon Type II - biosynthesis - genetics
Interleukin-2 - biosynthesis - genetics
Interleukin-4 - biosynthesis - genetics
Lung - immunology - metabolism
Ovalbumin - immunology
RNA, Messenger - genetics
Rats
Rats, Inbred BN
Research Support, Non-U.S. Gov't
Time Factors
Abstract
The events subsequent to antigen challenge in allergic asthmatics involve the synthesis of pro-inflammatory cytokines. However, little is known how cytokine gene activation prior to allergen challenge may influence this series of events, nor how cytokine gene expression is related to antigen-induced alterations in lung function. Using a novel in vitro explant technique, we hypothesized that the local expression of cytokines influenced the development of antigen-induced late-onset airway responses, and that alterations in cytokine messenger ribonucleic acid (mRNA) expression were associated with antigen-induced changes in airway luminal area. Explants were prepared from excised lungs of ovalbumin-sensitized Brown-Norway rats. Airways were challenged by direct application of ovalbumin or an irrelevant control antigen. Cryostat sections of explants were used for in situ hybridization and mRNA for interleukin (IL)-2, IL-4 and interferon (IFN)-gamma were detected using radiolabelled probes. We found that the presence of high numbers of cells expressing IFN-gamma and IL-2 mRNA within the airways attenuated the development of antigen-induced late airway responses in sensitized rat lung explants. Furthermore, we observed that cytokine mRNA for IL-4 was significantly increased following allergen exposure in sensitized lung explants exhibiting late airway responses. This study implicates the local expression of interferon-gamma and interleukin-2 messenger ribonucleic acid in the failure of sensitized rat lung explants to exhibit late airway responses, and provides evidence linking local interleukin-4 messenger ribonucleic acid expression to the sequelae of events occurring as a result of antigen exposure within the airways.
PubMed ID
9596114 View in PubMed
Less detail

8 records – page 1 of 1.