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Experimental autoimmune myasthenia gravis may occur in the context of a polarized Th1- or Th2-type immune response in rats.

https://arctichealth.org/en/permalink/ahliterature57544
Source
J Immunol. 1999 Jun 15;162(12):7189-97
Publication Type
Article
Date
Jun-15-1999
Author
A. Saoudi
I. Bernard
A. Hoedemaekers
B. Cautain
K. Martinez
P. Druet
M. De Baets
J C Guéry
Author Affiliation
Institut National de la Santé et de la Recherche Médicale, Unit 28, Université Paul Sabatier, Hôpital Purpan, Toulouse, France. abdelhadi@saoudi@purpan.insermfr
Source
J Immunol. 1999 Jun 15;162(12):7189-97
Date
Jun-15-1999
Language
English
Publication Type
Article
Keywords
Animals
Antibodies, Monoclonal - administration & dosage
Comparative Study
Female
Genetic Predisposition to Disease - immunology
Immunophenotyping
Incidence
Injections, Intraperitoneal
Interferon Type II - antagonists & inhibitors - immunology
Lymphocyte Activation - immunology
Male
Myasthenia Gravis - epidemiology - genetics - immunology - physiopathology
Rats
Rats, Inbred BN
Rats, Inbred Lew
Receptors, Cholinergic - immunology
Research Support, Non-U.S. Gov't
Th1 Cells - immunology - metabolism
Th2 Cells - immunology - metabolism
Torpedo
Abstract
Experimental autoimmune myasthenia gravis (EAMG) is a T cell-dependent, Ab-mediated autoimmune disease induced in rats by a single immunization with acetylcholine receptor (AChR). Although polarized Th1 responses have been shown to be crucial for the development of mouse EAMG, the role of Th cell subsets in rat EAMG is not well established. In the present work we show that while the incidence and severity of EAMG are similar in Lewis (LEW) and Brown-Norway (BN) rats, strong differences are revealed in the immune response generated. Ag-specific lymph node cells from LEW rats produced higher amounts of IL-2 and IFN-gamma than BN lymph node cells, but expressed less IL-4 mRNA. IgG1 and IgG2b anti-AChR isotype predominated in BN and LEW rats, respectively, confirming the dichotomy of the immune response observed between the two strains. Furthermore, although IL-12 administration or IFN-gamma neutralization strongly influenced the Th1/Th2 balance in BN rats, it did not affect the disease outcome. These data demonstrate that a Th1-dominated immune response is not necessarily associated with disease severity in EAMG, not only in rats with disparate MHC haplotype but also in the same rat strain, and suggest that in a situation where complement-fixing Ab can be generated as a consequence of either Th1- or Th2-mediated T cell help, deviation of the immune response will not be an adequate strategy to prevent this Ab-mediated autoimmune disease.
PubMed ID
10358165 View in PubMed
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