Skip header and navigation

Refine By

2 records – page 1 of 1.

Skin-infiltrating T cells and cytokine expression in Icelandic horses affected with insect bite hypersensitivity: a possible role for regulatory T cells.

https://arctichealth.org/en/permalink/ahliterature101906
Source
Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):63-74
Publication Type
Article
Date
Mar-15-2011
Author
Mareike Heimann
Jozef Janda
Olöf G Sigurdardottir
Vilhjalmur Svansson
Jolanta Klukowska
Claudia von Tscharner
Marcus Doherr
Hans Broström
Lisa S Andersson
Sigurjón Einarsson
Eliane Marti
Sigurbjörg Torsteinsdottir
Author Affiliation
Institute for Experimental Pathology, University of Iceland, Keldur, v/Vesturlandsveg, 112 Reykjavík, Iceland.
Source
Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):63-74
Date
Mar-15-2011
Language
English
Publication Type
Article
Keywords
Animals
Antigens, CD4 - analysis
Antigens, CD8 - analysis
Biopsy - veterinary
CD4-Positive T-Lymphocytes - metabolism
CD8-Positive T-Lymphocytes - metabolism
Ceratopogonidae - immunology
Cytokines - analysis - biosynthesis
Enzyme-Linked Immunosorbent Assay - veterinary
Forkhead Transcription Factors - analysis - biosynthesis
Horses - immunology
Hypersensitivity, Immediate - blood - etiology - immunology - veterinary
Immunoglobulin E - blood
Insect Bites and Stings - blood - immunology - veterinary
Interleukin-13 - analysis - biosynthesis
Lymphocyte Count - veterinary
Pruritus - blood - etiology - veterinary
Reverse Transcriptase Polymerase Chain Reaction - veterinary
Skin - pathology
T-Lymphocytes, Regulatory - metabolism
Abstract
Equine insect bite hypersensitivity (IBH) is a seasonally recurrent, pruritic skin disorder caused by an IgE-mediated reaction to salivary proteins of biting flies, predominantly of the genus Culicoides. The aim of this study was to define T cell subsets and cytokine profile in the skin of IBH-affected Icelandic horses with particular focus on the balance between T helper (Th) 1, Th2 and T regulatory (Treg) cells. Distribution and number of CD4+, CD8+ and Forkhead box P3 (FoxP3)+ T cells were characterized by immunohistochemical staining in lesional and non-lesional skin of moderately and severely IBH-affected horses (n=14) and in the skin of healthy control horses (n=10). Using real-time quantitative reverse transcription-polymerase chain reaction, mRNA expression levels of Th2 cytokines (Interleukin (IL)-4, IL-5, IL-13), Th1 cytokines (Interferon-?), regulatory cytokines (Transforming Growth Factor ß1, IL-10) and the Treg transcription factor FoxP3 were measured in skin and blood samples. Furthermore, Culicoides nubeculosus specific serum IgE levels were assessed. Lesions of IBH-affected horses contained significantly higher numbers of CD4+ cells than skin of healthy control horses. Furthermore, the total number of T cells (CD4+ and CD8+) was significantly increased in lesional compared to non-lesional skin and there was a tendency (p=0.07) for higher numbers of CD4+ cells in lesional compared to non-lesional skin. While the number of FoxP3+ T cells did not differ significantly between the groups, the ratio of Foxp3 to CD4+ cells was significantly lower in lesions of severely IBH-affected horses than in moderately affected or control horses. Interestingly, differences in FoxP3 expression were more striking at the mRNA level. FoxP3 mRNA levels were significantly reduced in lesional skin, compared both to non-lesional and to healthy skin and were also significantly lower in non-lesional compared to healthy skin. Expression levels of IL-13, but not IL-4 or IL-5, were significantly elevated in lesional and non-lesional skin of IBH-affected horses. IL-10 levels were lower in lesional compared to non-lesional skin (p=0.06) and also lower (p=0.06) in the blood of IBH-affected than of healthy horses. No significant changes were observed regarding blood expression levels of Th1 and Th2 cytokines or FoxP3. Finally, IBH-affected horses had significantly higher Culicoides nubeculosus specific serum IgE levels than control horses. The presented data suggest that an imbalance between Th2 and Treg cells is a characteristic feature in IBH. Treatment strategies for IBH should thus aim at restoring the balance between Th2 and Treg cells.
PubMed ID
21168921 View in PubMed
Less detail

[The manifestations of the immediate bioincompatibility reaction in patients undergoing restorative heart operations]

https://arctichealth.org/en/permalink/ahliterature54307
Source
Fiziol Zh. 1998;44(4):10-4
Publication Type
Article
Date
1998
Author
V S Shevchenko
Author Affiliation
Institute of Cardiovascular Surgery, Academy of Medical Sciense, Ukraine.
Source
Fiziol Zh. 1998;44(4):10-4
Date
1998
Language
Ukrainian
Publication Type
Article
Keywords
Autoantibodies - blood
Biocompatible Materials - adverse effects
Blood Proteins - analysis
Coronary Artery Bypass
Coronary Disease - blood - surgery
Endocarditis - blood - surgery
English Abstract
Heart Valve Prosthesis Implantation
Humans
Hypersensitivity, Immediate - blood - etiology
Immunodiffusion
Immunoelectrophoresis
Precipitins - blood
Time Factors
Abstract
The generation in plasma the anodic autoprecipitating protein, or autoprecipitin (AAP), as the bioincompatibility reaction under influence of a number of alien components during cardiac operations with using cardiopulmonary bypass, heterotopic vascular autotransplants and artificial valve implants was studied in 257 patients. Selective formation of the AAPs in result of their inductors effect was discovered by the methods of immunodiffusion and immunoelectrophoresis in blood of patients within operation periods and early postoperation hours. Repeated and reiterated generation of the AAPs due to bioincompatibility in patients with coronarosclerosis and endocarditis, undergoing prosthetic cardiac operations may favour developing reaction of immediate hypersensitivity with complications and has prognostic value.
PubMed ID
9669169 View in PubMed
Less detail