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Activation of flavonoid biosynthesis by solar radiation in bilberry ( Vaccinium myrtillus L) leaves.

https://arctichealth.org/en/permalink/ahliterature9570
Source
Planta. 2004 Mar;218(5):721-8
Publication Type
Article
Date
Mar-2004
Author
Laura Jaakola
Kaisu Määttä-Riihinen
Sirpa Kärenlampi
Anja Hohtola
Author Affiliation
Department of Biology/Botany, University of Oulu, POB 3000, 90014, Oulu, Finland. laura.jaakola@oulu.fi
Source
Planta. 2004 Mar;218(5):721-8
Date
Mar-2004
Language
English
Publication Type
Article
Keywords
Acclimatization - physiology - radiation effects
Acyltransferases - genetics - metabolism
Alcohol Oxidoreductases - genetics - metabolism
Flavonoids - biosynthesis - radiation effects
Fruit - metabolism - radiation effects
Gene Expression Regulation, Enzymologic - radiation effects
Gene Expression Regulation, Plant - radiation effects
Mixed Function Oxygenases - genetics - metabolism
Oxygenases - genetics - metabolism
Phenylalanine Ammonia-Lyase - genetics - metabolism
Plant Leaves - metabolism - radiation effects
Research Support, Non-U.S. Gov't
Sunlight
Vaccinium myrtillus - genetics - metabolism - radiation effects
Abstract
The effect of solar radiation on flavonoid biosynthesis was studied in bilberry ( Vaccinium myrtillus L.) leaves. Expression of flavonoid pathway genes of bilberry was studied in the upper leaves of bilberry, exposed to direct sunlight, in the shaded leaves growing lower in the same plants and in fruits. Bilberry-specific digoxigenin-dUTP-labeled cDNA fragments of five genes from the general phenylpropanoid pathway coding phenylalanine ammonia-lyase and from the flavonoid pathway coding chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase were used as probes in gene expression analysis. Anthocyanins, catechins, proanthocyanidins, flavonols and hydroxycinnamic acids from the leaves and fruits were identified and quantified using high-performance liquid chromatography combined with a diode array detector. An increase in the expression of the studied flavonoid pathway genes was observed in leaves growing under direct sun exposure. Also, the concentrations of anthocyanins, catechins, flavonols and hydroxycinnamic acids were higher in the leaves exposed to direct sunlight. However, the concentration of polymeric procyanidins was lower in sun-exposed leaves, whereas that of prodelphinidins was slightly increased. The results give further support for the protective role of flavonoids and hydroxy cinnamic acids against high solar radiation in plants. Also, the roles of different flavonoid compounds as a defense against stress caused by sun exposure is discussed.
PubMed ID
14666422 View in PubMed
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