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Amaltheys: A fluorescence-based analyzer to assess cheese milk denatured whey proteins.

https://arctichealth.org/en/permalink/ahliterature273576
Source
J Dairy Sci. 2015 Oct;98(10):6668-77
Publication Type
Article
Date
Oct-2015
Author
Pierre Lacotte
Franck Gomez
Floriane Bardeau
Sabine Muller
Abdelhaq Acharid
Xavier Quervel
Philippe Trossat
Inès Birlouez-Aragon
Source
J Dairy Sci. 2015 Oct;98(10):6668-77
Date
Oct-2015
Language
English
Publication Type
Article
Keywords
Animals
Cheese - analysis
Denmark
Fluorometry - methods
Food Quality
Milk - chemistry
Whey Proteins - analysis
Abstract
The cheese industry faces many challenges to optimize cheese yield and quality. A very precise standardization of the cheese milk is needed, which is achieved by a fine control of the process and milk composition. Thorough analysis of protein composition is important to determine the amount of protein that will be retained in the curd or lost in the whey. The fluorescence-based Amaltheys analyzer (Spectralys Innovation, Romainville, France) was developed to assess pH 4.6-soluble heat-sensitive whey proteins (sWP*) in 5 min. These proteins are those that can be denatured upon heat-treatment and further retained in the curd after coagulation. Monitoring of sWP* in milk and subsequent adaptation of the process is a reliable solution to achieve stable cheese yield and quality. Performance of the method was evaluated by an accredited laboratory on a 0 to 7 g/L range. Accuracy compared with the reference Kjeldahl method is also provided with a standard error of 0.25 g/L. Finally, a 4-mo industrial trial in a cheese plant is described, where Amaltheys was used as a process analytical technology to monitor sWP* content in ingredients and final cheese milk. Calibration models over quality parameters of final cheese were also built from near-infrared and fluorescence spectroscopic data. The Amaltheys analyzer was found to be a rapid, compact, and accurate device to help implementation of standardization procedures in the dairy industry.
PubMed ID
26210276 View in PubMed
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Analytical performance of time-resolved fluorometry-based Innotrac Aio! cardiac marker immunoassays.

https://arctichealth.org/en/permalink/ahliterature53547
Source
Scand J Clin Lab Invest. 2003;63(1):55-64
Publication Type
Article
Date
2003
Author
P. Hedberg
J. Valkama
M. Puukka
Author Affiliation
Laboratory of Oulu University Hospital, Department of Clinical Chemistry, University of Oulu, Finland. pirjo.hedberg@ppshp.fi
Source
Scand J Clin Lab Invest. 2003;63(1):55-64
Date
2003
Language
English
Publication Type
Article
Keywords
Biological Markers - blood
Clinical Laboratory Techniques - instrumentation - methods
Coronary Arteriosclerosis - blood - diagnosis
Creatine Kinase - blood
Creatine Kinase, MB Form
Fluorometry - instrumentation
Humans
Immunoassay - methods
Isoenzymes - blood
Myoglobin - blood
Point-of-Care Systems
Reproducibility of Results
Troponin I - blood
Abstract
The results of an evaluation of the Innotrac Aio! cardiac markers are presented. This system is based on dry-chemistry, time-resolved fluorometry. All assay-specific reagents are dry-coated into assay-specific cups, and only the generic assay buffer is required. The levels of precision attained with pooled serum samples and control materials were acceptable for cTnI and CK-MB. Myoglobin assay showed higher CV, 5.6-9.5%. The linearity studies were performed in concentration ranges of 0.1-76 microg/L for cTnI, 0.7-450 microg/L for CK-MB and 0.6-1500 microg/L for myoglobin. The markers were found to be linear within the ranges tested. The correlation coefficient between the Aio! and AxSYM cTnI assays was 0.960, and the slope was 0.07. The correlation coefficients between the Aio! and AxSYM CK-MB and myoglobin assays were 0.995 and 0.971, respectively. They involved some differences in the measured concentrations (Aio! CK-MB was about 9% higher than AxSYM CK-MB, and Aio! myoglobin was 19% higher than AxSYM). Comparative studies with all the markers, using EDTA whole blood and lithium heparin plasma specimens and lithium heparin whole blood and plasma, yielded the following results: the slopes were close to 1.0 for all correlations, with the exception of that between CK-MB EDTA whole blood and lithium heparin (0.83). High correlation coefficients were obtained (> or = 0.97). The carryover results for all the cardiac markers were good, 0.0%, 0.0%, and 0.3% for cTnI, CK-MB, and myoglobin, respectively. The analytical detection limits were 0.01 microg/L for cTnI, 0.8 microg/L for CK-MB and 0.5 microg/L for myoglobin. The stability of the analytes in the lithium heparin samples at room temperature was also studied and was found to be decreased by from 10% (myoglobin and CK-MB) to 17% (cTnI) in 8 h. Innotrac Aio! provides a rapid and easy quantitative measurement of cardiac TnI, CK-MB, and myoglobin within
PubMed ID
12729070 View in PubMed
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Archival bone marrow samples: suitable for multiple biomarker analysis.

https://arctichealth.org/en/permalink/ahliterature265165
Source
Appl Immunohistochem Mol Morphol. 2015 Jan;23(1):71-7
Publication Type
Article
Date
Jan-2015
Author
Bendik Lund
Laeya A Najmi
Agata Wesolowska-Andersen
Veslemøy M Landsem
Kirsten K Rasmussen
Louise Borst
Ramneek Gupta
Kjeld Schmiegelow
Helge Klungland
Source
Appl Immunohistochem Mol Morphol. 2015 Jan;23(1):71-7
Date
Jan-2015
Language
English
Publication Type
Article
Keywords
Adolescent
Biopsy
Bone Banks - standards
Bone Marrow - physiology
Child
Child, Preschool
DNA - analysis - isolation & purification
Denmark
Female
Fluorometry
Genome - genetics
Genotype
Humans
Male
Microsatellite Repeats - genetics
Norway
Pilot Projects
Polymorphism, Single Nucleotide
Precursor Cell Lymphoblastic Leukemia-Lymphoma - diagnosis - genetics
Specimen Handling
Spectrophotometry
Tumor Markers, Biological - analysis
Abstract
AB Archival samples represent a significant potential for genetic studies, particularly in severe diseases with risk of lethal outcome, such as in cancer. In this pilot study, we aimed to evaluate the usability of archival bone marrow smears and biopsies for DNA extraction and purification, whole genome amplification (WGA), multiple marker analysis including 10 short tandem repeats, and finally a comprehensive genotyping of 33,683 single nucleotide polymorphisms (SNPs) with multiplexed targeted next-generation sequencing. A total of 73 samples from 21 bone marrow smears and 13 bone marrow biopsies from 18 Danish and Norwegian childhood acute lymphoblastic leukemia patients were included and compared with corresponding blood samples. Samples were grouped according to the age of sample and whether WGA was performed or not. We found that measurements of DNA concentration after DNA extraction was dependent on detection method and that spectrophotometry overestimated DNA amount compared with fluorometry. In the short tandem repeat analysis, detection rate dropped slightly with longer fragments. After WGA, this drop was more pronounced. Samples stored for 0 to 3 years showed better results compared with samples stored for 4 to 10 years. Acceptable call rates for SNPs were detected for 7 of 42 archival samples. In conclusion, archival bone marrow samples are suitable for DNA extraction and multiple marker analysis, but WGA was less successful, especially when longer fragments were analyzed. Multiple SNP analysis seems feasible, but the method has to be further optimized.
PubMed ID
25621358 View in PubMed
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Assessment of intra-individual variation in prostate-specific antigen levels in a biennial randomized prostate cancer screening program in Sweden.

https://arctichealth.org/en/permalink/ahliterature16963
Source
Prostate. 2005 Nov 1;65(3):216-21
Publication Type
Article
Date
Nov-1-2005
Author
Laila Bruun
Charlotte Becker
Jonas Hugosson
Hans Lilja
Anders Christensson
Author Affiliation
Department of Nephrology and Transplantation, Lund University, University Hospital (UMAS), Malmö, Sweden. laila.bruun@klkemi.mas.lu.se
Source
Prostate. 2005 Nov 1;65(3):216-21
Date
Nov-1-2005
Language
English
Publication Type
Article
Keywords
Aged
Cohort Studies
Fluorometry
Humans
Individuality
Male
Mass Screening
Middle Aged
Predictive value of tests
Prostate-Specific Antigen - blood
Prostatic Neoplasms - blood - epidemiology
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Sweden - epidemiology
Abstract
BACKGROUND: The degree of variability in prostate-specific antigen (PSA) measurements is important for interpreting test results in screening programs, and particularly for interpreting the significance of changes between repeated tests. This study aimed to determine the long-term intra-individual variation for PSA in healthy men. METHODS: A randomly selected cohort of men in a biennial prostate cancer screening program (ERSPC) conducted in Sweden from 1995-1996 to 2001-2002. We studied men who had total PSA (tPSA) levels
PubMed ID
15948137 View in PubMed
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Cellular drug sensitivity in MLL-rearranged childhood acute leukaemia is correlated to partner genes and cell lineage.

https://arctichealth.org/en/permalink/ahliterature17095
Source
Br J Haematol. 2005 Apr;129(2):189-98
Publication Type
Article
Date
Apr-2005
Author
J. Palle
B M Frost
E. Forestier
G. Gustafsson
P. Nygren
M. Hellebostad
O G Jonsson
J. Kanerva
K. Schmiegelow
R. Larsson
G. Lönnerholm
Author Affiliation
Department of Women's and Children's Health, University Children's Hospital, Uppsala, Sweden. josefine.palle@akademiska.se
Source
Br J Haematol. 2005 Apr;129(2):189-98
Date
Apr-2005
Language
English
Publication Type
Article
Keywords
Acute Disease
Adolescent
Antineoplastic Agents - pharmacology
Cell Lineage
Child
Child, Preschool
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 9
Comparative Study
Cytarabine - pharmacology
Cytotoxicity Tests, Immunologic
DNA-Binding Proteins - genetics
Doxorubicin - pharmacology
Drug Resistance, Neoplasm - genetics
Female
Fluorometry
Gene Rearrangement
Glucocorticoids - pharmacology
Humans
Infant
Infant, Newborn
Leukemia, Lymphocytic, Acute, L1 - genetics - immunology
Leukemia, Myeloid - genetics - immunology
Male
Myeloid-Lymphoid Leukemia Protein
Prospective Studies
Proto-Oncogenes - genetics
Research Support, Non-U.S. Gov't
Statistics, nonparametric
Transcription Factors - genetics
Translocation, Genetic
Abstract
Rearrangements in the 11q23 region, the site of the mixed lineage leukaemia (MLL) gene, are found in both childhood acute myeloid (AML) and lymphoblastic (ALL) leukaemia. We studied the in vitro drug resistance by the fluorometric microculture cytotoxicity assay (FMCA) in 132 children with AML and 178 children with ALL (aged 0-17 years). In AML, children with t(9;11) (n = 10) were significantly more sensitive to cytarabine (P
PubMed ID
15813846 View in PubMed
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40 records – page 1 of 4.