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Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks.

https://arctichealth.org/en/permalink/ahliterature289442
Source
Vopr Virusol. 2017; 62(1):30-5
Publication Type
Comparative Study
Journal Article
Author
N M Pukhovskaya
Olga V Morozova
N B Belozerova
S V Bakhmetyeva
N P Vysochina
N I Zdanovskaya
L I Ivanov
Source
Vopr Virusol. 2017; 62(1):30-5
Language
English
Publication Type
Comparative Study
Journal Article
Keywords
Aedes - virology
Amino Acid Sequence
Animals
Animals, Newborn
Disease Vectors
Dogs
Encephalitis Viruses, Tick-Borne - classification - genetics - isolation & purification
Encephalitis, Tick-Borne - epidemiology - transmission - virology
Far East - epidemiology
Genome, Viral
Genotype
Humans
Ixodes - virology
Mice
Mice, Inbred ICR
Nucleic Acid Conformation
Phylogeny
RNA, Viral - chemistry - genetics - isolation & purification
Rodentia - virology
Sequence Alignment
Sequence Homology, Amino Acid
Siberia - epidemiology
Abstract
The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier.
PubMed ID
29323844 View in PubMed
Less detail

Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks.

https://arctichealth.org/en/permalink/ahliterature289600
Source
Vopr Virusol. 2017; 62(1):30-5
Publication Type
Comparative Study
Journal Article
Author
N M Pukhovskaya
Olga V Morozova
N B Belozerova
S V Bakhmetyeva
N P Vysochina
N I Zdanovskaya
L I Ivanov
Source
Vopr Virusol. 2017; 62(1):30-5
Language
English
Publication Type
Comparative Study
Journal Article
Keywords
Aedes - virology
Amino Acid Sequence
Animals
Animals, Newborn
Disease Vectors
Dogs
Encephalitis Viruses, Tick-Borne - classification - genetics - isolation & purification
Encephalitis, Tick-Borne - epidemiology - transmission - virology
Far East - epidemiology
Genome, Viral
Genotype
Humans
Ixodes - virology
Mice
Mice, Inbred ICR
Nucleic Acid Conformation
Phylogeny
RNA, Viral - chemistry - genetics - isolation & purification
Rodentia - virology
Sequence Alignment
Sequence Homology, Amino Acid
Siberia - epidemiology
Abstract
The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier.
PubMed ID
29323844 View in PubMed
Less detail