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The apoptosis inducing effects of Sutherlandia spp. extracts on an oesophageal cancer cell line.

https://arctichealth.org/en/permalink/ahliterature132365
Source
J Ethnopharmacol. 2011 Oct 11;137(3):1250-60
Publication Type
Article
Date
Oct-11-2011
Author
Nicola B Skerman
Annie M Joubert
Marianne J Cronjé
Author Affiliation
Department of Biochemistry, University of Johannesburg, APK Campus, PO Box 524, Auckland Park 2006, South Africa.
Source
J Ethnopharmacol. 2011 Oct 11;137(3):1250-60
Date
Oct-11-2011
Language
English
Publication Type
Article
Keywords
Adenosine Triphosphate - metabolism
Amino Acid Chloromethyl Ketones - pharmacology
Antineoplastic Agents, Phytogenic - isolation & purification - pharmacology
Apoptosis - drug effects
Caspase 3 - metabolism
Caspase 7 - metabolism
Caspase Inhibitors
Cell Line, Tumor
Cell Shape - drug effects
Cell Survival - drug effects
Cysteine Proteinase Inhibitors - pharmacology
Cytochromes c - metabolism
Dose-Response Relationship, Drug
Esophageal Neoplasms - metabolism - pathology
Fabaceae - chemistry
Flow Cytometry
Humans
Leukocytes, Mononuclear - drug effects - pathology
Phosphatidylserines - metabolism
Plant Extracts - isolation & purification - pharmacology
Plants, Medicinal
Protein Transport
Spectrometry, Fluorescence
Time Factors
Abstract
Oesophageal cancer is the ninth most common cancer in the world and the second most common cancer among South African men. It also has one of the lowest possibilities of cure, with the 5-year survival rate estimated to be only 10% overall. Sutherlandia frutescens, or the "cancer bush", is a medicinal plant indigenous to southern Africa that is believed to have anti-cancer and anti-proliferative properties. The aim of this study was to investigate the potential apoptosis-inducing effects of two S. frutescens extracts and one Sutherlandia tomentosa extract on the SNO oesophageal cancer cell line.
Cell viability and morphology of SNO cells were evaluated following exposure to the extracts. Apoptotic markers including cytochrome c translocation and phosphatidylserine externalisation were quantified by flow cytometry. The activity of caspases 3 and 7 was evaluated with spectrofluorometry. Apoptosis was evaluated in the presence of the pan-caspase inhibitor, Z-VAD-fmk. The effect of the extracts was compared to non-cancerous peripheral blood mononuclear cells (PBMCs).
Time- and dose-response studies were conducted to establish treatment conditions of 2.5 and 5mg/ml of crude plant extracts. Microscopy studies revealed that S. frutescens- and S. tomentosa-treated SNO cells had morphological features characteristic of apoptosis. Annexin V/propidium iodide flow cytometry confirmed that the extracts do, in fact, induce apoptosis in the SNO cells. Caspase inhibition studies seem to indicate that extracts A (S. frutescens (L.) R. Br. subsp. microphylla from Colesberg), B (S. frutescens (L.) R. Br. subsp. microphylla from Platvlei) and C (S. tomentosa Eckl. & Zeyh from Stil Bay) are able to induce caspase-dependent as well as -independent cell death. The S. frutescens and S. tomentosa extracts were found to be more cytotoxic to cancerous SNO cells when compared to the PBMCs.
S. frutescens and S. tomentosa extracts show promise as apoptosis-inducing anti-cancer agents.
PubMed ID
21824511 View in PubMed
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Esophageal cancer phospholipids correlated with histopathologic findings: a 31P NMR study.

https://arctichealth.org/en/permalink/ahliterature3994
Source
NMR Biomed. 1999 Jun;12(4):184-8
Publication Type
Article
Date
Jun-1999
Author
T E Merchant
B D Minsky
G Y Lauwers
P M Diamantis
T. Haida
T. Glonek
Author Affiliation
Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, USA. thomas.merchant@stjude.org
Source
NMR Biomed. 1999 Jun;12(4):184-8
Date
Jun-1999
Language
English
Publication Type
Article
Keywords
Esophageal Neoplasms - metabolism - pathology
Humans
Magnetic Resonance Spectroscopy
Phospholipids - metabolism
Abstract
We analyzed 36 esophageal tumor specimens for phospholipid content using phosphorus nuclear magnetic resonance spectroscopy (31P NMR) and correlated the individual phospholipid profiles with specific clinical and histopathologic features. Among the 18 phospholipids identified in the esophageal tumor specimens, the mean mole percentage concentration of dimethylphosphatidylethanolamine, lysoalkylacylphosphatidylcholine, lysophosphatidic acid, lysophosphatidylcholine (deacylated at the glycerol-1 carbon), and lysoethanolamine plasmalogen correlated with pathologic T stage, nuclear grade, or the presence of lymphatic invasion. 31P NMR produces well-dispersed phospholipid spectra and a precise determination of phospholipid relative mole percentages. These data provide a statistical correlation between histopathologic features and molecules known to play an important role in cellular activities and processes unique to malignant tissues.
PubMed ID
10421909 View in PubMed
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