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Upregulation of tumour endothelial marker-8 by interleukin-1beta and its impact in IL-1beta induced angiogenesis.

https://arctichealth.org/en/permalink/ahliterature179610
Source
Int J Mol Med. 2004 Jul;14(1):75-80
Publication Type
Article
Date
Jul-2004
Author
Khaled A Rmali
Mahir A A Al-Rawi
Christian Parr
Malcolm C A Puntis
Wen G Jiang
Author Affiliation
Metastasis and Angiogenesis Research Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, UK. rmali@cf.ac.uk
Source
Int J Mol Med. 2004 Jul;14(1):75-80
Date
Jul-2004
Language
English
Publication Type
Article
Keywords
Endothelium, Vascular - chemistry - cytology - drug effects
Humans
Interleukin-1 - pharmacology
Membrane Proteins
Neoplasm Proteins
Neovascularization, Pathologic - metabolism
RNA, Messenger - analysis
Receptors, Cell Surface - analysis - biosynthesis - genetics
Tumor Markers, Biological - metabolism
Up-Regulation
Abstract
Tumour endothelial marker-8 (TEM-8) has been found to be selectively upregulated in tumour-associated endothelial cells and is implicated in tumour specific angiogenesis. Specific factors, indigenous to tissues and tumours that regulate the TEM-8 mechanism in angiogenesis are not defined. We report for the first time that interleukin-1beta induces the expression of TEM-8 in endothelial cells. Human vascular endothelial cells (HECV), which strongly express IL-1beta receptor (as revealed by RT-PCR, Western blotting), increased the level of TEM-8 expression following stimulation with IL-1beta (as revealed by conventional and quantitative RT-PCR). Using a newly developed antibody to human TEM-8, we have further demonstrated that IL-1beta significantly raised the level of TEM-8 at the protein level, as revealed by Western blotting. In vitro tubule forming assay, revealed that IL-1beta significantly induced the formation of capillary-like tubules from the HECV cells, accompanied by an increase in TEM-8 expression. It is concluded that IL-1beta is a powerful regulator of the expression of TEM-8 in vascular endothelial cells. Our results suggest an important pathway through which IL-1beta regulates tumour-associated angiogenesis.
PubMed ID
15202019 View in PubMed
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