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188 records – page 1 of 19.

[Affinity sorbents with tripeptide morpholine ligands for isolation of proteases]

https://arctichealth.org/en/permalink/ahliterature33976
Source
Bioorg Khim. 1997 Nov;23(11):868-76
Publication Type
Article
Date
Nov-1997
Author
A V Kuznetsova
G N Rudenskaia
A M Bogacheva
T L Voiushina
V M Stepanov
Author Affiliation
Moscow State University, Chemistry Department, Russia.
Source
Bioorg Khim. 1997 Nov;23(11):868-76
Date
Nov-1997
Language
Russian
Publication Type
Article
Keywords
Animals
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Endopeptidases - isolation & purification
English Abstract
Humans
Ligands
Morpholines - chemistry
Oligopeptides - chemical synthesis - chemistry
Sepharose - analogs & derivatives - chemistry
Substrate Specificity
Abstract
New affine sorbents were synthesized involving tripeptide morpholides H-Ala-Ala-Leu-Mrp and H-D-Ala-Leu-Arg-Mrp as ligands that mimic substrates of subtilisin-like proteases and kallikrein, respectively. These were used for the isolation and purification of several proteases: trypsin, pepsin, alpha-chymotrypsin, thrombin, kallikrein, and termitase and were also efficient in the isolation of proteolytic enzymes from complex mixtures, such as the urine of children suffering from glomerulonephritis, hepatopancreas of Kamchatka crab, and dandelion roots. The ligands are competitive inhibitors of a number of proteases, and therefore, they were supposed to interact with the substrate binding sites in these enzymes.
PubMed ID
9518426 View in PubMed
Less detail

Age-associated changes in MAPK activation in fast- and slow-twitch skeletal muscle of the F344/NNiaHSD X Brown Norway/BiNia rat model.

https://arctichealth.org/en/permalink/ahliterature82974
Source
Exp Gerontol. 2006 Feb;41(2):205-14
Publication Type
Article
Date
Feb-2006
Author
Mylabathula D B
Rice K M
Wang Z.
Uddemarri S.
Kinnard R S
Blough E R
Author Affiliation
Department of Biological Sciences, Marshall University, Huntington, WV 25755-1090, USA.
Source
Exp Gerontol. 2006 Feb;41(2):205-14
Date
Feb-2006
Language
English
Publication Type
Article
Keywords
Aging - physiology
Animals
Blotting, Western
Electrophoresis, Polyacrylamide Gel
MAP Kinase Signaling System - physiology
Male
Mitogen-Activated Protein Kinase Kinases - metabolism
Models, Animal
Muscle Contraction
Muscle Fibers, Fast-Twitch - enzymology - physiology
Muscle Fibers, Slow-Twitch - enzymology - physiology
Phosphorylation
Rats
Rats, Inbred BN
Rats, Inbred F344
Abstract
We compared the tissue content, basal phosphorylation, and stretch-induced phosphorylation of the mitogen-activated protein kinase (MAPK) members; extracellular-signal-regulated kinases (ERK 1/2), p38, and c-Jun NH2-terminal kinase (JNK) in the fast-twitch extensor digitorium longus (EDL) and slow-twitch soleus of young adult (6 month), aged (30 month), and very aged (36 month) F344/NNiaHSD X Brown Norway/BiNia (F344/NXBN) rats. The expression and basal phosphorylation of the ERK 1/2, p38, and JNK MAPK proteins were regulated differently with aging in the EDL and soleus. Stretch induced significant phosphorylation of each signaling molecule in both muscle types of young adult and aged animals. In the very aged animals, stretch stimulated ERK 1/2 MAPK phosphorylation; however, EDL stretch failed to induce JNK MAPK phosphorylation, while soleus stretch was unable to induce the phosphorylation of p38 MAPK. The results suggest that skeletal muscle mechanotransduction processes are affected in very aged F344/NXBN rats and that aging alters load-induced signaling in fast- and slow-twitch muscle types differently.
PubMed ID
16378702 View in PubMed
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[A genetic pool study of the population of the Republic of Tyva using genetic markers for the search for associations with tuberculosis]

https://arctichealth.org/en/permalink/ahliterature69550
Source
Probl Tuberk. 1996;(4):38-41
Publication Type
Article
Date
1996
Author
L E Pospelov
A G Matrakshin
K N Tsoi
L S Erdynieva
B K Kuderek
Source
Probl Tuberk. 1996;(4):38-41
Date
1996
Language
Russian
Publication Type
Article
Keywords
Blood Donors
Blood Proteins - genetics
Chi-Square Distribution
Comparative Study
Electrophoresis, Polyacrylamide Gel - statistics & numerical data
English Abstract
Gene Pool
Genetic markers
Genotype
HLA Antigens - genetics
Humans
Reference Values
Risk factors
Siberia
Tuberculosis, Pulmonary - genetics
Abstract
Eighty three Tyvin patients with local pulmonary tuberculosis and 295 healthy donors of the same nationality were examined. They are resided in the central area of Tyva (in Kyzyl and its vicinities). In addition, 132 healthy Tyvin-Todjins were examined. Tuberculosis mortality was found to be associated with the antigen HLA-B15 in the Tyvins living in the central area of the Republic of Tyva (Kyzyl). The incidence of HLA antigens and polymorphic protein locus genotypes varies in different areas of the Republic of Tyva.
PubMed ID
9026803 View in PubMed
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Age-related changes in renal function, membrane protein metabolism, and Na,K-ATPase activity and abundance in hypokalemic F344 x BNF(1) rats.

https://arctichealth.org/en/permalink/ahliterature61838
Source
Gerontology. 1999 Sep-Oct;45(5):254-64
Publication Type
Article
Author
S. Eiam-Ong
S. Sabatini
Author Affiliation
Department of Internal Medicine, The Combined Program in Nephrology and Renal Physiology, Texas Tech University Health Sciences Center, Lubbock, Tex. 79430, USA.
Source
Gerontology. 1999 Sep-Oct;45(5):254-64
Language
English
Publication Type
Article
Keywords
Age Factors
Aging
Animals
Cell Membrane - enzymology - metabolism
Diet
Electrophoresis, Polyacrylamide Gel
Hypokalemia - blood - physiopathology
Immunoblotting
Kidney - physiology
Kidney Tubules, Proximal - enzymology
Male
Membrane Proteins - metabolism
Microvilli - metabolism
Na(+)-K(+)-Exchanging ATPase - metabolism
Potassium - administration & dosage - blood
Potassium Deficiency - blood
Rats
Rats, Inbred BN
Rats, Inbred F344
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Abstract
BACKGROUND: Potassium depletion is a common electrolyte abnormality in elderly humans, usually as a consequence of diuretic use or poor oral intake. Hypokalemia is associated with a number of changes in renal function and an increase in some renal membrane transporters; its growth-promoting effect in young animals is well known. With aging, the renal adaptation to a number of challenges is often diminished. We hypothesized that aging is related to decreases in renal function, renal membrane protein metabolism, as well as Na, K-ATPase protein abundance and activity in both control animals as well as in those with potassium depletion. OBJECTIVE: We examined the effects of dietary-induced hypokalemia in true-aged nonobese rats (30 months old) on renal function, cortical brush border membrane (BBM) and basolateral membrane (BLM) protein metabolism, and Na,K-ATPase protein abundance and activity. We compared the results obtained to those seen in their 4-month-old counterparts similarly treated. Methods: Young (4-month-old) and senescent (30-month-old) male Fisher 344 x Brown-Norway F(1) rats (F344 x BNF(1)) were fed either a normal or potassium-deficient diet for 7 days. At 24 h, the U-(14)C-leucine incorporation was measured for determination of protein metabolism in renal BBM and BLM. Cortical BLM vesicle and microdissected proximal convoluted tubule (PCT) Na, K-ATPase activities were determined along with Western blot analysis of the cortical BLM alpha(1) subunit of Na,K-ATPase. Metabolic and renal function parameters were also examined. RESULTS: Hypokalemia caused hyperbicarbonatemia, hyperglycemia, and azotemia, but only in the senescent animals. The aged control rats had a higher basal level of urine volume, ammonium excretion, and fractional excretion of chloride. By contrast, aging in the F344 x BNF(1) rats was associated with a decrease in plasma aldosterone (by 35%) and phosphate (by 40%) levels as compared with their young controls. Hypokalemia resulted in a significant reduction of plasma aldosterone and a rise in muscle sodium concentration in both age groups; it significantly increased renal BBM and BLM protein concentrations in the young group, while these parameters remained unchanged in the senescent rats. The aged potassium-depleted animals showed a 14% decrease in BBM protein biosynthesis, but there were no changes in the young hypokalemic rats. Both potassium-depleted elderly and young rats had a significant reduction (by 33%) in BLM protein biosynthesis. Hypokalemia significantly increased the Na, K-ATPase activity in both cortical BLM vesicles and in microdissected PCT. The percentage increase in microdissected PCT segments (Na,K-ATPase activity) in elderly potassium-depleted animals was significantly less than that seen in hypokalemic young ones. Aging, per se, was associated with decreased basal microdissected PCT Na,K-ATPase activity in control animals. Hypokalemia had no effect on cortical BLM alpha(1) subunit Na, K-ATPase protein abundance in either age group. CONCLUSIONS: The present study provides the first evidence in nonobese aged rats as to the metabolic parameters, renal function, renal cortical membrane protein metabolism, and transporter Na,K-ATPase activity and abundance during potassium depletion. The aged nonobese F344 x BNF(1) rats responded differently from their young nonobese counterparts following potassium depletion. These differences may contribute substantially to the effects often encountered in elderly humans receiving diuretics or having a poor dietary potassium intake.
PubMed ID
10460986 View in PubMed
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[A modification to the chloroform-methanol method of isolating the matrix protein of the influenza virus]

https://arctichealth.org/en/permalink/ahliterature10805
Source
Mikrobiol Z. 1998 May-Jun;60(3):91-5
Publication Type
Article
Author
A I Gordienlko
Iu S Krivoshein
A I Gordienko
Author Affiliation
Crimean Medical Institute, Department of Microbiology, Sympheropol', Ukraine.
Source
Mikrobiol Z. 1998 May-Jun;60(3):91-5
Language
Russian
Publication Type
Article
Keywords
Animals
Antigens, Viral - analysis - isolation & purification
Chloroform
Comparative Study
Electrophoresis, Polyacrylamide Gel - methods
English Abstract
Humans
Immune Sera - isolation & purification
Immunoenzyme Techniques
Indicators and Reagents
Influenza A virus - immunology
Methanol
Rabbits
Solvents
Ultracentrifugation
Viral Matrix Proteins - immunology - isolation & purification
Abstract
A modification of chloroform-methanol method of isolation of M1 protein (M1P) of human influenza A virus allowed increasing 1.6 times the yield of the target product and decreasing 6.4 times the amount of organic solvents. It has been shown that M1 protein isolated by the modified method did not differ (as to its physicochemical characteristics and antigenic activity) from M1 protein isolated by means of the initial method.
PubMed ID
9785805 View in PubMed
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Amyloid fibril composition is related to the phenotype of hereditary transthyretin V30M amyloidosis.

https://arctichealth.org/en/permalink/ahliterature92323
Source
J Pathol. 2008 Oct;216(2):253-61
Publication Type
Article
Date
Oct-2008
Author
Ihse E.
Ybo A.
Suhr Ob
Lindqvist P.
Backman C.
Westermark P.
Author Affiliation
Department of Genetics and Pathology, Uppsala University, Sweden.
Source
J Pathol. 2008 Oct;216(2):253-61
Date
Oct-2008
Language
English
Publication Type
Article
Keywords
Abdominal Fat - chemistry - pathology
Adult
Age of Onset
Amino Acid Substitution
Amyloid - analysis - genetics - ultrastructure
Amyloidosis, Familial - genetics - pathology - ultrasonography
Blotting, Western - methods
Cardiomyopathies - genetics - pathology
Echocardiography
Electrophoresis, Polyacrylamide Gel - methods
Female
Heart Septal Defects, Ventricular - genetics - pathology - ultrasonography
Humans
Male
Methionine - genetics
Middle Aged
Prealbumin - chemistry - genetics - metabolism
Sweden
Valine - genetics
Abstract
Swedish familial systemic amyloidosis with polyneuropathy (FAP) depends on a mutation leading to a methionine-for-valine substitution in transthyretin. The disease appears with different clinical manifestations, including age of onset and involvement of the heart. Liver transplantation is currently the only curative treatment, but progressive cardiomyopathy may occur post-transplant. Two amyloid deposition patterns have previously been described in the heart. In one, the amyloid consists partially of transthyretin fragments and is weakly stainable by Congo red, while in the other, only full-length molecules are found and the fibrils have a strong affinity for Congo red. The present study aimed to see whether these morphological and biochemical variations have clinical implications. Subcutaneous adipose tissue biopsies were taken from 33 patients with Val30Met FAP and examined by microscopy, electrophoresis and western blot. Clinical data included age, sex, duration of disease and echocardiographic determination of the interventricular septum (IVS) thickness. It was found that fibrils composed of only full-length transthyretin were associated with early age of onset (44.8 +/- 12.9 years), no clinical cardiac involvement and a strong affinity for Congo red. In contrast, presence of transthyretin fragments in the amyloid was associated with late age of onset (67.3 +/- 7.0 years), signs of cardiac involvement and weak Congo red staining. For each individual, the same molecular type of amyloid was found in different organs. This is the first report showing that variations in clinical appearance of familial ATTR amyloidosis are associated with specific structural differences in the amyloid fibrils, and therefore may have a molecular cause. The molecular type of amyloid can be determined from a subcutaneous fat tissue biopsy.
PubMed ID
18729067 View in PubMed
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Analysis of allele distribution for six short tandem repeat loci in the French Canadian population of Québec.

https://arctichealth.org/en/permalink/ahliterature207194
Source
J Forensic Sci. 1997 Nov;42(6):1147-53
Publication Type
Article
Date
Nov-1997
Author
L. Busque
D. Desmarais
S. Provost
J W Schumm
Y. Zhong
R. Chakraborty
Author Affiliation
Centre de Recherche Guy Bernier, Hôpital Maisonneuve-Rosemont, Montréal, Québec, Canada.
Source
J Forensic Sci. 1997 Nov;42(6):1147-53
Date
Nov-1997
Language
English
Publication Type
Article
Keywords
Adult
Alleles
Child
DNA - analysis
Electrophoresis, Polyacrylamide Gel
European Continental Ancestry Group - genetics
Female
France - ethnology
Gene Frequency - genetics
Genetic Markers - genetics
Humans
Male
Paternity
Polymerase Chain Reaction
Polymorphism, Genetic
Quebec - epidemiology
Repetitive Sequences, Nucleic Acid - genetics
Sequence Analysis, DNA
Abstract
Short tandem repeat (STR) loci represent a rich source of highly polymorphic markers in the human genome which are useful for the purposes of forensic identification and determination of biological relatedness of individuals. Here, as a part of an ongoing extensive study, we report the analysis of a multilocus genotype survey of 642 to 870 chromosomes in the French Canadian Caucasian population of Québec at six STR loci. The loci HUMCSF1PO, HUMTPOX, HUMTH01, HUMF13A01, HUMFESFPS, and HUMvWA were typed using two multiplex polymerase chain reactions (PCR). Amplified DNA samples were subsequently analyzed by polyacrylamide gel electrophoresis followed by silver staining. The heterozygote frequencies of the loci range from 0.614 to 0.820 (0.661 to 0.818 expected) and the number of alleles from 7 to 12 per locus. Although statistically significant deviation from Hardy-Weinberg expectations of genotype frequencies was noted at some loci by one or more tests, in general, the genotype frequencies are well estimated from the product of allele frequencies at all loci. The most frequent six-locus genotype is expected to occur in the French Canadian population with a frequency of 3.50 by 10(-5) and together, these six loci have an average probability of discrimination of 0.9999985. The study presented here indicates that these six STR loci are informative genetic markers for identity testing purposes in the French Canadian Caucasian population of Québec.
PubMed ID
9397560 View in PubMed
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Anaphylactic reaction caused by neoallergens in heated pecan nut.

https://arctichealth.org/en/permalink/ahliterature213707
Source
Allergy. 1995 Dec;50(12):988-91
Publication Type
Article
Date
Dec-1995
Author
K. Malanin
M. Lundberg
S G Johansson
Author Affiliation
Medical Center of Lappeenranta, Finland.
Source
Allergy. 1995 Dec;50(12):988-91
Date
Dec-1995
Language
English
Publication Type
Article
Keywords
Allergens - adverse effects
Anaphylaxis - etiology - immunology
Child
Electrophoresis, Polyacrylamide Gel
Female
Finland
Humans
Nuts - immunology
Radioallergosorbent Test
Skin Tests
Abstract
An atopic girl experienced an anaphylactic reaction after eating cookies containing pecan nuts. Investigations revealed that she had developed IgE antibodies exclusively specific against allergenic determinants present in aged or heated pecan nuts, but not in fresh pecans. These neoallergenic determinants were located on protein(s) with a molecular weight of approximately 15 kDa. Neoallergens appearing during heating or storing of foods may be important in some anaphylactic reactions.
PubMed ID
8834830 View in PubMed
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188 records – page 1 of 19.