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Cell-surface-bound nucleic acids: Free and cell-surface-bound nucleic acids in blood of healthy donors and breast cancer patients.

https://arctichealth.org/en/permalink/ahliterature17606
Source
Ann N Y Acad Sci. 2004 Jun;1022:221-7
Publication Type
Article
Date
Jun-2004
Author
Pavel P Laktionov
Svetlana N Tamkovich
Elena Yu Rykova
Olga E Bryzgunova
Andrey V Starikov
Nina P Kuznetsova
Valentin V Vlassov
Author Affiliation
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. lakt@niboch.nsc.ru
Source
Ann N Y Acad Sci. 2004 Jun;1022:221-7
Date
Jun-2004
Language
English
Publication Type
Article
Keywords
Breast Neoplasms - blood - diagnosis - genetics - pathology
Chelating Agents - pharmacology
Comparative Study
DNA - blood - isolation & purification - metabolism
Deoxyribonucleases - metabolism
Edetic Acid - pharmacology
Electrophoresis, Agar Gel
Erythrocytes - drug effects
Female
Fibroadenoma - blood - diagnosis - genetics - pathology
Humans
Leukocytes - drug effects
Neoplasm Staging
Nucleic Acids - blood - isolation & purification - metabolism
RNA - blood - isolation & purification - metabolism
Research Support, Non-U.S. Gov't
Ribonucleases - metabolism
Trypsin - pharmacology
Abstract
Concentrations of extracellular DNA and RNA in the blood of healthy donors and patients with malignant and nonmalignant breast tumors were investigated. Cell-surface-bound extracellular DNA and RNA were detached by PBS-EDTA treatment or mild trypsin treatment of erythrocytes and leukocytes. In healthy donors, almost all extracellular nucleic acids (98%) are bound at the surface of blood cells. In the blood of cancer patients, extracellular nucleic acids were found in plasma and not at the cell surface. In patients with nonmalignant breast tumors, extracellular nucleic acids were found both at the surface of blood cells and in plasma. In healthy donors, the cell-surface-bound DNA is represented by 20-kbp DNA fragments and smaller fragments that varied in amounts in different fractions.
PubMed ID
15251964 View in PubMed
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